Detailed information NTG0711738

Bioinformatically predicted

Overview

• Name: addB
• Type: Machinery gene
• Locus tag: NPA28_RS05770
• Genome accession: NZ_CP101718
• Coordinates: 1170693..1174193 (+)
• Length: 1166 a.a.
• NCBI ID: WP_217827178.1
• Uniprot ID: -
• Organism: Bacillus halotolerans strain S-5
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1165693..1179193

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NPA28_RS05750 (NPA28_05750)	-	1166547..1167748 (-)	1202	Protein_1075	MDR family MFS transporter	-
NPA28_RS05755 (NPA28_05755)	-	1167864..1169594 (+)	1731	WP_217827179.1	ABC transporter substrate-binding protein	-
NPA28_RS05760 (NPA28_05760)	-	1169628..1169942 (-)	315	WP_044156484.1	four-helix bundle copper-binding protein	-
NPA28_RS05765 (NPA28_05765)	-	1170071..1170508 (-)	438	WP_044156482.1	ferritin family protein	-
NPA28_RS05770 (NPA28_05770)	addB	1170693..1174193 (+)	3501	WP_217827178.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
NPA28_RS05775 (NPA28_05775)	addA	1174180..1177884 (+)	3705	WP_217827177.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
NPA28_RS05780 (NPA28_05780)	sbcD	1177956..1179134 (+)	1179	WP_106294530.1	exonuclease subunit SbcD	-

Sequence

Protein

• Length: 1166 a.a.
• Molecular weight: 134725.76 Da
• Isoelectric Point: 5.4792

>NTDB_id=711738 NPA28_RS05770 WP_217827178.1 1170693..1174193(+) (addB) [Bacillus halotolerans strain S-5]
MGAEFLVGRSGSGKTKLIIDSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDTGGMIRAQVFSFSRLAWRVLQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKSGFTAQVERMLTEFKRYCLEPEDIRRMADSGTASEYRGERVLSE
KLHDLSILYQQMEKSLEDQYLHSEDYLTLLAEHIPQAEDLKGAHVYVDGFYQFTPQEFRVLEQLMLQAEHITFSLTADKP
SHEGEPQELDLFRMTGKTYYRLHQAAKELNLDITYKELDESKRHKRAPELAHLEAQYEARPVIPFAEKQEAFTVMQAANR
RAELEGIAREIHSLVREKGYRYKDMAILARQPKDYKDMVKEVFADYEIPYFIDGKTSMLNHPLVEFIRSSLDVLKGNWRY
EAVFRCVKTELLFPLNQPKAKVREQVDQLENYCIAYGIKGDRWTKGDRFHYRRFVSLDDEFAQTDQEIEMENMLNDTRDW
IVPPLFQLQKRMKKAKTVQEKAEALYRYLEETDVPLKLDQERQRAEEDGRIIEAQQHQQAWDAVIQLLEEFVEMMGEDDI
SLDLFQQMMETGAESLTFSLIPPALDQVFVGNMDLSRIYGTSCTFVIGANDGVLPARPDENGVLSDDDREWLKTIGIELS
SGGRERLLDEHFLIYMALSSPSDRLYVTYPIADAEGKTLLPSIVIKRLEELFPQHRERLLTNEPEQVSDEEQLMYLVNKS
VAQSYTASQLRLWTREYEISDVWWSAYNALMNEPDRLESKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLQLKERQFFKLEAPDIGQLFHSSLKLISDRLREHKIDWRDLTKEQCELFSYDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFKLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSLDWLGMKATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEAIRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFELLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCAFKSVCQFDESLEENEYRLLKAEKDKTVLDWIKKEADGNEHS

Nucleotide

• Length: 3501 bp

>NTDB_id=711738 NPA28_RS05770 WP_217827178.1 1170693..1174193(+) (addB) [Bacillus halotolerans strain S-5]
TTGGGAGCAGAGTTTTTAGTAGGCAGGTCCGGGAGTGGAAAAACGAAGCTGATCATCGACAGTATTCAAGATGAACTGCG
CCGGGCTCCATTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTGCTAAAA
CGCCTGATACGGGCGGCATGATTCGTGCTCAAGTCTTCAGCTTTTCACGACTGGCCTGGCGCGTCCTCCAGCATACGGGA
GGAATGAGCAGGCCGTTTCTTACGAGCACGGGCGTACAGATGCTCCTGCGGAAGCTCATTGAAGAGCACAAACAGGAGTT
TAAAGTCTATCAAAAAGCAAGTGATAAAAGCGGTTTTACCGCACAGGTAGAACGGATGCTGACTGAGTTTAAGAGATACT
GTCTGGAACCGGAAGATATTCGCCGGATGGCGGATAGCGGAACAGCTTCCGAGTATCGGGGGGAACGTGTTTTATCTGAA
AAGCTTCATGATTTATCGATCCTGTACCAGCAGATGGAAAAAAGCCTTGAGGATCAGTATCTTCATTCTGAGGATTATTT
GACACTATTGGCAGAGCATATCCCGCAGGCGGAAGATTTGAAAGGAGCACATGTCTATGTAGACGGCTTTTATCAGTTTA
CACCGCAGGAATTCAGGGTGTTAGAGCAGCTTATGCTTCAGGCAGAGCATATCACGTTTTCACTCACAGCGGACAAGCCG
TCACATGAGGGGGAGCCGCAGGAACTTGATTTGTTCAGAATGACGGGAAAAACGTATTATCGCTTGCACCAGGCTGCAAA
GGAACTGAATCTGGACATCACCTATAAAGAGCTTGATGAATCAAAGCGGCACAAACGCGCACCGGAATTGGCCCATTTAG
AAGCACAGTATGAAGCGCGTCCGGTTATTCCGTTTGCTGAGAAACAAGAGGCTTTTACTGTGATGCAGGCCGCTAACAGA
CGAGCAGAGCTGGAAGGTATTGCCCGGGAAATCCACAGTCTTGTCAGAGAGAAGGGCTACCGTTATAAGGATATGGCGAT
TCTAGCGCGTCAGCCGAAAGACTATAAGGATATGGTCAAGGAAGTATTTGCAGATTACGAGATTCCTTATTTTATTGACG
GGAAAACTTCTATGCTGAACCACCCGTTAGTAGAATTTATCCGGTCAAGCCTTGACGTATTAAAGGGAAATTGGCGTTAT
GAAGCGGTGTTCCGCTGCGTGAAAACCGAACTGTTATTCCCGCTTAACCAGCCGAAGGCCAAGGTAAGAGAGCAGGTTGA
TCAGCTGGAGAATTACTGTATCGCTTACGGGATCAAAGGCGATCGCTGGACAAAGGGCGATCGTTTCCATTACAGACGTT
TTGTGTCGTTAGATGATGAATTTGCGCAGACCGATCAAGAAATCGAAATGGAAAACATGCTGAATGACACGCGCGATTGG
ATTGTTCCGCCGCTGTTCCAGCTGCAGAAACGAATGAAAAAAGCAAAAACGGTTCAAGAGAAGGCGGAGGCGCTTTACCG
TTATTTAGAAGAGACGGATGTGCCGCTGAAGCTGGATCAGGAAAGACAGCGGGCTGAGGAAGACGGCAGAATCATTGAAG
CACAGCAGCACCAGCAGGCGTGGGATGCAGTCATTCAGCTGCTTGAGGAGTTTGTGGAAATGATGGGCGAAGATGACATT
TCTCTCGATTTGTTTCAGCAAATGATGGAGACCGGTGCAGAGTCGCTGACATTCTCTCTCATCCCTCCGGCGCTTGATCA
GGTGTTTGTCGGCAATATGGATTTGTCCAGAATATACGGCACCTCATGCACCTTTGTGATCGGGGCAAACGACGGCGTCC
TGCCGGCTCGCCCTGACGAAAACGGGGTGCTGTCGGATGATGATCGTGAGTGGCTGAAGACGATAGGTATTGAGCTTTCC
TCCGGCGGGCGGGAGCGTCTGCTTGATGAGCATTTCCTCATCTATATGGCGCTTTCAAGCCCTTCTGACCGCCTTTATGT
GACGTATCCGATTGCTGATGCGGAAGGGAAAACGCTTTTGCCGTCGATTGTAATCAAGCGGCTGGAAGAACTGTTTCCGC
AGCATCGTGAGCGTCTATTGACAAATGAACCGGAGCAGGTCAGTGATGAGGAACAGCTTATGTACCTTGTCAATAAAAGC
GTGGCACAATCATATACCGCTAGCCAGCTCAGATTATGGACGAGGGAATATGAGATCAGTGATGTCTGGTGGAGCGCGTA
CAATGCGCTGATGAATGAACCTGACAGGCTGGAGTCGAAAAAGCTTTTCTCTAGCCTGTTTTTCCGAAATGAAGTCAAGC
AGCTTGAACGCTCTGTATCGAGACAGCTCTATGGGGAACGCATTCAAGGGAGCGTGTCGAGGATGGAAACCTTTAACGCT
TGTCCGTTCTCCCATTTTGCGTCCCACGGGCTGCAGCTGAAGGAACGTCAATTTTTTAAGCTTGAAGCACCGGATATCGG
GCAGCTCTTTCATTCGAGTTTAAAACTGATTTCAGACCGGCTGCGCGAGCACAAAATAGATTGGCGCGACCTAACGAAGG
AGCAGTGCGAGCTGTTTTCTTATGATGCGGTCGAGCGGCTGGCGCCGAAACTGCAAAAAGAAATTCTGCTCAGTTCAAAC
AGACATTATTACGTGAAGGAAAAACTGCAAAAAATTGTGACACGCGTGTCCGGCATTTTAAGCGAGCATGCGAAAGCGAG
CGGATTCGTGCCGATCGGCCTTGAATTGGGCTTTGGCGGAAAGGGCCCGCTCCCGCCGCTTACCTTTAAGCTGAAAAACG
GCTGTACGATGGAGCTCGTCGGGCGGATTGACCGCGTGGATAAAGCGGAAAGCTCTAAAGGCCTGCTCCTCCGAATTGTC
GACTATAAATCCAGCGATAAAGGCCTTGACTTGGCGGAAGTGTATTACGGACTTGCGCTGCAAATGCTGACGTATCTGGA
TCTATCGATTACACATTCGTTAGACTGGCTAGGAATGAAAGCCACGCCGGCCGGAGTGCTGTATTTCCATATTCATGACC
CGATGATACAATCGAATCTTCCGCTCGGACTTGATGAAATTGAACAGGAAATTTTTAAGAAATTTAAGATGAAGGGCCTC
CTCCTCGGTGATCAGGAAGCGATTCGCCTCATGGATACAACCCTTCAAGAAGGAAGATCAAATATCATCAATGCGGGCCT
GAAGAAAGACGGCTCACTCAGATCGGATTCAGCGGCAGTCGGTGAAAAAGAATTTGAGCTGTTGACGAAGCATGTGCGCC
GCACCTTCCAAGAAGCAGGCGAGCAAATCACTGACGGGCGCGTATCCATTGAGCCGTATAAAATGAAGAACAAGACGCCT
TGCACATACTGTGCGTTCAAATCAGTATGCCAATTTGATGAATCATTAGAAGAAAACGAGTATCGTCTGTTAAAGGCCGA
AAAGGACAAGACGGTACTTGATTGGATAAAGAAGGAGGCTGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (306-668)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (790-1131)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addB	Bacillus subtilis subsp. subtilis str. 168	94.254	100	0.943