Detailed information    

insolico Bioinformatically predicted

Overview

Name   mutS/mutS2   Type   Machinery gene
Locus tag   POF63_RS08415 Genome accession   NZ_CP116708
Coordinates   1638012..1640351 (-) Length   779 a.a.
NCBI ID   WP_001060329.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain CNCTC 10/84 sCas9     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1633012..1645351
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF63_RS08385 (POF63_08385) ssb 1633290..1633781 (-) 492 WP_000609586.1 single-stranded DNA-binding protein Machinery gene
  POF63_RS08390 (POF63_08390) rpsF 1633793..1634080 (-) 288 WP_001151773.1 30S ribosomal protein S6 -
  POF63_RS08395 (POF63_08395) mutY 1635143..1636297 (+) 1155 WP_162473108.1 A/G-specific adenine glycosylase -
  POF63_RS08400 (POF63_08400) - 1636474..1637067 (+) 594 WP_000402394.1 helix-turn-helix transcriptional regulator -
  POF63_RS08405 (POF63_08405) trxA 1637113..1637445 (-) 333 WP_001932060.1 thioredoxin -
  POF63_RS08410 (POF63_08410) - 1637508..1638008 (-) 501 WP_000446809.1 phosphatase PAP2 family protein -
  POF63_RS08415 (POF63_08415) mutS/mutS2 1638012..1640351 (-) 2340 WP_001060329.1 endonuclease MutS2 Machinery gene
  POF63_RS08420 (POF63_08420) - 1640436..1640978 (-) 543 WP_000949954.1 CvpA family protein -
  POF63_RS08425 (POF63_08425) zapA 1640981..1641292 (-) 312 WP_000448289.1 cell division protein ZapA -
  POF63_RS08430 (POF63_08430) rnhC 1641403..1642296 (+) 894 WP_001092531.1 ribonuclease HIII -
  POF63_RS08435 (POF63_08435) lepB 1642312..1642905 (+) 594 WP_000657514.1 signal peptidase I -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 87668.73 Da        Isoelectric Point: 6.3004

>NTDB_id=711284 POF63_RS08415 WP_001060329.1 1638012..1640351(-) (mutS/mutS2) [Streptococcus agalactiae strain CNCTC 10/84 sCas9]
MNNKILEQLEFNKVKELILPYLKTEQSQEELSGLEPMTEAPKIEKSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLEL
SADLNIQELLAIKKVLQSSSDMIHFYSDLDNVSFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTVYIEPRAVVTLNEEITQLRADER
HEESRILHAFSDLLRPHVATIRNNAWILGHLDFVRAKYLFMSDNKATIPEISNDSTLALINVRHPLLSNPVANDLHFDQD
LTAIVITGPNTGGKTIMLKTLGLAQLMGQSGLPVLADKGSKIAVFNNIFADIGDEQSIEQSLSTFSSHMTHIVSILNEAD
HNSLVLFDELGAGTDPQEGASLAMAILEHLRLSNIKTMATTHYPELKAYGIETNFVENASMEFDAETLSPTYRFMQGVPG
RSNAFEIASRLGLAPFIVKQAKQMTDSDSDVNRIIEQLEAQTLETRRRLDHIKEVEQENLKFNRAVKKLYNEFSHERDKE
LEKIYQEAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKKIKAARAPRIGDDIIVT
SYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFTLVRVQEEQKVKSKQINVVKKADSSGPRARLDLRGKRYEEAMQELDN
FIDQALLNNMGQVDIIHGIGTGVIREGVTKYLRRNKHVKHFAYAPQNAGGSGATIVTLG

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=711284 POF63_RS08415 WP_001060329.1 1638012..1640351(-) (mutS/mutS2) [Streptococcus agalactiae strain CNCTC 10/84 sCas9]
ATGAATAACAAGATTTTAGAACAGTTAGAATTTAACAAAGTTAAGGAATTGATATTACCTTATCTCAAGACAGAACAATC
ACAAGAAGAATTATCTGGGCTGGAGCCGATGACGGAGGCTCCTAAAATAGAAAAAAGTTTTAATGAAATTTCTGACATGG
AACAGATTTTTGTTGAACATCACTCATTTGGCATAGTCAGCCTAAGTTCAATCTCTGAGAGTTTAAAACGCTTAGAGCTT
TCAGCTGATCTTAATATTCAAGAACTTTTGGCTATCAAAAAAGTTTTACAGAGTTCTTCGGATATGATTCACTTTTATTC
TGATTTGGATAATGTTTCTTTCCAATCTTTGGATCGTTTGTTTGAAAATTTGGAACAATTCCCTAATCTGCAAGGGTCTT
TTCAAGCTATCAATGATGGTGGTTTTTTAGAACATTTTGCGAGTCCAGAATTAGAGCGTATCCGTCGTCAATTAACAAAC
AGTGAACGACGGGTTCGTCAGATTTTACAGGATATGCTTAAGGAAAAAGCAGAGCTTTTATCAGAGAATCTAATCGCTAG
TCGTAGTGGACGAAGTGTCCTACCAGTAAAAAATACTTATCGGAATCGTATTTCTGGTGTGGTTCATGACATCTCTTCTT
CAGGAAGTACTGTTTATATTGAGCCTCGTGCTGTAGTTACACTAAACGAAGAGATAACGCAGCTTAGAGCTGACGAACGT
CATGAAGAAAGTCGTATTTTACACGCATTTTCAGACTTGTTAAGACCCCATGTCGCCACTATTAGAAATAATGCATGGAT
TCTTGGGCATCTTGATTTTGTTAGGGCTAAATATCTTTTTATGTCTGATAATAAGGCGACGATACCTGAGATTTCTAATG
ACAGCACGTTAGCATTAATCAATGTTCGTCATCCTCTGTTAAGTAACCCTGTGGCTAATGACTTACATTTTGATCAAGAT
TTAACTGCAATTGTCATCACTGGTCCCAATACTGGTGGTAAGACGATTATGTTAAAAACACTCGGTTTAGCACAATTAAT
GGGACAGTCTGGTTTGCCAGTTTTAGCGGATAAAGGTAGTAAAATTGCAGTATTTAACAATATCTTTGCAGATATTGGCG
ATGAGCAATCTATTGAACAAAGTCTATCAACTTTTTCTAGTCATATGACGCACATAGTTAGTATTTTAAACGAGGCTGAC
CACAATAGTTTAGTTCTCTTTGATGAACTGGGAGCAGGAACGGATCCTCAAGAAGGTGCTAGTTTGGCTATGGCTATTTT
AGAACATCTTAGGTTAAGTAATATCAAAACGATGGCGACCACACACTATCCAGAATTAAAAGCTTATGGGATTGAGACAA
ATTTTGTAGAGAATGCGAGCATGGAATTTGATGCCGAAACGCTTAGCCCTACGTATCGCTTTATGCAAGGAGTTCCTGGA
CGATCAAATGCATTTGAAATTGCTTCTCGCCTTGGTTTAGCTCCATTTATTGTTAAACAAGCTAAGCAGATGACAGATTC
TGACTCAGATGTTAACCGTATTATTGAACAGTTAGAGGCACAGACACTTGAGACACGTAGAAGACTGGATCATATTAAAG
AAGTTGAACAAGAAAACCTCAAATTCAATCGTGCGGTTAAGAAACTCTATAATGAATTTTCACATGAGCGCGATAAAGAG
TTAGAAAAAATCTATCAAGAAGCTCAAGAAATTGTAGATATGGCTTTGAATGAGAGTGATACTATCTTAAAAAAACTCAA
TGATAAGAGCCAATTAAAACCTCACGAAATTATAGATGCTAAGGCACAAATAAAAAAATTAGCACCTCAAGTTGATTTAT
CAAAAAATAAAGTCTTAAATAAGGCTAAAAAAATCAAGGCAGCTCGTGCCCCTAGAATTGGTGATGATATTATAGTGACT
AGCTATGGACAGCGAGGTACCTTAACTAGTCAATTAAAAGATGGACGTTGGGAAGCACAAGTGGGAATTATCAAAATGAC
ATTAACACAAGATGAATTTACCCTCGTTAGAGTCCAAGAAGAACAGAAAGTCAAAAGTAAACAGATTAATGTGGTTAAAA
AGGCTGATAGTTCTGGACCAAGAGCTCGACTTGATCTTAGAGGTAAAAGATACGAAGAAGCTATGCAAGAGTTAGATAAT
TTTATTGATCAAGCATTGCTTAACAATATGGGACAAGTTGATATCATTCATGGTATTGGTACAGGCGTTATCCGTGAGGG
AGTGACAAAATACCTTCGTCGTAATAAGCACGTTAAACATTTTGCTTATGCCCCACAAAATGCAGGGGGATCTGGTGCCA
CAATTGTAACGTTAGGGTAG

Domains

Predicted by InterProScan.

MutS domain V (MutS_V)

(324-505)

Smr domain (Smr)

(704-778)

MutS2 and Smr-associated SH3 domain (MSSS)

(633-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  mutS/mutS2 Bacillus subtilis subsp. subtilis str. 168

40.38

100

0.41