Detailed information    

insolico Bioinformatically predicted

Overview

Name   recD/recD2/recDB   Type   Machinery gene
Locus tag   POF65_RS08790 Genome accession   NZ_CP116706
Coordinates   1720087..1722507 (+) Length   806 a.a.
NCBI ID   WP_000451609.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain A909 sCas9     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1715087..1727507
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF65_RS08770 (POF65_08770) - 1717489..1718031 (-) 543 WP_000949954.1 CvpA family protein -
  POF65_RS08775 (POF65_08775) zapA 1718034..1718345 (-) 312 WP_000448289.1 cell division protein ZapA -
  POF65_RS08780 (POF65_08780) rnhC 1718456..1719349 (+) 894 WP_001092527.1 ribonuclease HIII -
  POF65_RS08785 (POF65_08785) lepB 1719365..1719958 (+) 594 WP_000657514.1 signal peptidase I -
  POF65_RS08790 (POF65_08790) recD/recD2/recDB 1720087..1722507 (+) 2421 WP_000451609.1 ATP-dependent RecD-like DNA helicase Machinery gene
  POF65_RS08795 (POF65_08795) - 1722621..1723103 (+) 483 WP_000255475.1 hypothetical protein -
  POF65_RS08800 (POF65_08800) dinB 1723174..1724268 (-) 1095 WP_000904560.1 DNA polymerase IV -
  POF65_RS08805 (POF65_08805) pflB 1724453..1726765 (+) 2313 WP_000246708.1 formate C-acetyltransferase -
  POF65_RS08810 (POF65_08810) - 1726866..1727240 (+) 375 WP_000931878.1 pyridoxamine 5'-phosphate oxidase family protein -

Sequence

Protein


Download         Length: 806 a.a.        Molecular weight: 91004.40 Da        Isoelectric Point: 4.7227

>NTDB_id=711069 POF65_RS08790 WP_000451609.1 1720087..1722507(+) (recD/recD2/recDB) [Streptococcus agalactiae strain A909 sCas9]
MEVFFTGTIERIIFENASNFFKILLLEIEDTDSDFDDVEVIITGTMADVIEGEEYTFWGTLTQHPKYGEQLQSVRYERAK
PTSGGLVKYFSSEQFKGIGKKTAQRIVELYGDNTIDKILESPEQLSTISGLSKINREAFIAKLKLNYGTEQVLAKLAEYG
LSNRAAIQIFDHYKEESLEVINENPYQLVEDIQGIGFKIADQLAEQVGIESDSPKRFRAAIIHTLVESSMEQGDTYIEAR
TLLEKTITLLEEARQIELDPSIVAKELTNLIAEDKVQHIGTKIFSNTLFFAEEGIKKNLQRILNQPLDKQLNHKDIDREI
RDIQKSLNIHYDNIQEKAIREALLSKVFILTGGPGTGKTTVINGIIEAYSELHHIDLNKNDIPIVLAAPTGRAARRMNEL
TGLPSATIHRHLGLNGDSDYQSLDDYLDCSLIIIDEFSMVDTWLANQLFDALDSHTQVIIVGDSDQLPSVGPGQVLADLL
NINALPHVKLEKIFRQSEESTIVTLANQMRQGFLPEDFTAKKADRSYFEASANIIPNMISKIVQSALKSGIEAHEIQILA
PMYRGQAGINNLNLIMQNLLNPLKDNNQFTFNDINFRIGDKVLHLVNDTELNVFNGDIGYITDLIPAKYTESKQDEIYMT
FDGQEVIYQRKEWLKITLAYAMSIHKSQGSEFQVVILPITRQSGRMLQRNLIYTAITRSKSKLILLGEIGAFDFAVKNEG
AKRNTYLIERFENKQEIANSQKIEDSSIDQKIDNTIINTSIPKTATPIEQTNLSKITYRLTEENYLTIDPMIGINQQDIS
AIFDSK

Nucleotide


Download         Length: 2421 bp        

>NTDB_id=711069 POF65_RS08790 WP_000451609.1 1720087..1722507(+) (recD/recD2/recDB) [Streptococcus agalactiae strain A909 sCas9]
ATGGAAGTATTTTTTACAGGAACCATTGAACGCATTATATTTGAAAATGCCAGCAATTTTTTTAAAATATTACTTCTGGA
AATCGAAGATACGGATAGCGATTTTGATGATGTAGAAGTAATTATTACTGGTACTATGGCCGACGTTATAGAGGGAGAAG
AATATACTTTTTGGGGAACACTAACCCAACATCCAAAATACGGCGAGCAACTACAAAGTGTACGCTATGAAAGAGCCAAG
CCTACTTCAGGTGGCCTTGTGAAATACTTTTCTAGCGAACAATTTAAAGGAATTGGTAAAAAGACTGCCCAACGCATCGT
AGAACTCTATGGAGATAATACCATTGATAAAATCCTTGAATCCCCTGAACAGTTAAGTACAATCTCAGGACTATCAAAAA
TTAATAGAGAAGCATTCATCGCTAAACTGAAACTCAATTATGGAACAGAGCAAGTTTTAGCTAAACTCGCTGAATATGGG
TTAAGCAATCGTGCTGCTATTCAAATATTTGATCACTATAAAGAAGAATCACTTGAAGTGATAAACGAAAATCCTTATCA
ACTTGTTGAGGATATCCAAGGTATCGGTTTTAAAATAGCTGACCAACTTGCTGAACAAGTCGGTATTGAAAGTGATTCTC
CTAAACGCTTTCGTGCTGCTATTATTCATACTTTAGTTGAGTCTTCTATGGAGCAAGGAGATACCTATATTGAGGCTCGT
ACTCTCTTGGAAAAAACCATTACTCTCCTTGAAGAAGCTCGTCAAATCGAACTAGATCCTTCAATTGTTGCTAAGGAATT
GACAAATTTAATTGCAGAAGATAAAGTGCAACATATTGGAACTAAAATTTTTAGTAATACTCTATTTTTTGCCGAAGAAG
GCATCAAAAAAAACTTACAACGAATACTAAATCAGCCTCTTGATAAACAACTAAATCACAAAGATATTGACCGTGAAATC
AGAGATATTCAAAAATCTTTAAATATCCACTACGATAATATCCAAGAAAAAGCTATTAGAGAAGCTTTACTAAGTAAGGT
ATTTATCTTGACTGGTGGCCCAGGAACAGGTAAAACAACAGTTATAAATGGTATTATTGAAGCCTATTCCGAGTTACATC
ATATTGATTTAAACAAAAATGATATTCCTATTGTACTTGCTGCTCCTACAGGTCGTGCTGCTAGACGCATGAATGAACTT
ACCGGTTTACCGAGTGCTACTATTCATAGGCATTTGGGATTAAACGGAGATAGTGACTATCAAAGTCTAGATGACTATCT
AGATTGTTCCTTAATTATCATTGATGAATTTTCAATGGTTGACACTTGGCTTGCTAATCAATTATTTGACGCTTTGGACT
CGCACACACAAGTTATTATTGTCGGAGATAGTGATCAATTACCGTCTGTAGGTCCAGGACAAGTTCTAGCAGATCTTTTA
AATATAAATGCACTTCCACACGTTAAACTTGAAAAGATTTTTAGACAATCAGAAGAATCAACTATTGTGACTTTAGCTAA
TCAAATGAGACAGGGCTTTTTGCCAGAGGACTTTACAGCTAAAAAAGCCGATCGCTCATACTTTGAAGCATCTGCTAATA
TCATCCCAAATATGATTTCTAAAATTGTTCAATCTGCACTTAAGAGCGGTATTGAAGCTCATGAAATTCAAATTTTAGCT
CCTATGTATCGCGGCCAAGCAGGTATTAATAATCTTAACCTTATCATGCAAAATCTCCTCAATCCATTAAAAGATAATAA
CCAATTTACTTTTAATGATATAAATTTCCGAATAGGAGATAAAGTGTTACATTTAGTCAACGATACAGAGCTTAATGTTT
TTAATGGGGATATTGGTTACATAACGGACCTTATACCAGCCAAATATACTGAGAGCAAGCAAGATGAGATTTATATGACT
TTTGACGGTCAAGAAGTTATCTATCAACGCAAAGAATGGCTTAAAATAACACTAGCCTATGCTATGAGTATTCATAAATC
ACAGGGTTCTGAATTTCAAGTTGTTATTCTGCCAATAACGAGACAGAGTGGTCGAATGTTACAGCGAAACCTTATTTATA
CAGCTATTACGAGATCTAAAAGTAAACTAATACTTTTAGGAGAAATTGGTGCATTTGATTTCGCTGTTAAAAACGAGGGG
GCAAAGCGCAATACTTATCTGATAGAAAGATTTGAAAACAAGCAAGAAATAGCTAATTCTCAAAAAATTGAAGATAGCTC
AATAGATCAAAAGATTGACAACACAATAATTAATACGTCCATTCCAAAAACAGCAACACCAATTGAGCAGACTAATCTTT
CAAAAATAACTTATCGCCTAACTGAAGAAAATTATTTAACAATCGATCCAATGATTGGTATTAATCAACAAGATATAAGT
GCAATTTTTGATAGTAAATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_30)

(332-520)

UvrD-like helicase C-terminal domain (UvrD_C_2)

(659-706)

ATP-dependent RecD-like DNA helicase SH3 domain (SH3_13)

(572-638)

Helix-hairpin-helix containing domain (HHH_RecD2)

(147-236)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recD/recD2/recDB Bacillus subtilis subsp. subtilis str. 168

43.658

92.928

0.406