Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DWB40_RS15735 Genome accession   NZ_CP101622
Coordinates   3411128..3412165 (-) Length   345 a.a.
NCBI ID   WP_004147099.1    Uniprot ID   A0AAP7J2J0
Organism   Stenotrophomonas maltophilia strain CYZ     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3406128..3417165
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DWB40_RS15710 - 3406660..3407109 (-) 450 WP_005408373.1 GNAT family N-acetyltransferase -
  DWB40_RS15715 - 3407206..3407418 (-) 213 WP_005408372.1 DUF4287 domain-containing protein -
  DWB40_RS15720 - 3407747..3408646 (+) 900 WP_062606848.1 LysR family transcriptional regulator -
  DWB40_RS15725 - 3408694..3409677 (-) 984 WP_062606621.1 aldo/keto reductase -
  DWB40_RS15730 pilU 3409885..3411027 (-) 1143 WP_005408369.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DWB40_RS15735 pilT 3411128..3412165 (-) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  DWB40_RS15740 - 3412253..3412930 (+) 678 WP_062606619.1 YggS family pyridoxal phosphate-dependent enzyme -
  DWB40_RS15745 proC 3412949..3413770 (+) 822 WP_005408367.1 pyrroline-5-carboxylate reductase -
  DWB40_RS15750 - 3413830..3414705 (-) 876 WP_062606617.1 LysR family transcriptional regulator -
  DWB40_RS15755 - 3414780..3415406 (+) 627 Protein_3100 short chain dehydrogenase -
  DWB40_RS15760 soxR 3415479..3415913 (-) 435 WP_005412484.1 redox-sensitive transcriptional activator SoxR -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38658.41 Da        Isoelectric Point: 6.6654

>NTDB_id=711068 DWB40_RS15735 WP_004147099.1 3411128..3412165(-) (pilT) [Stenotrophomonas maltophilia strain CYZ]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPLFREVIQQPQGLILVTGPTGSGKSTTLAAMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMMTLDQHL
QDLVKRSLITRNQAREYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=711068 DWB40_RS15735 WP_004147099.1 3411128..3412165(-) (pilT) [Stenotrophomonas maltophilia strain CYZ]
ATGGATATCGCCGAACTGCTGGCGTTTTCCGTAAAGAACAAAGCGTCCGACCTGCACCTGTCCGCAGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTTCGCCGGATCAACATCCCAGCCCTGGACCACAAGCAGGTCCATGCGCTGGTGTACGACA
TCATGTCCGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAAGTGGACTTCTCCTTCGAGATTCCATCGCTGGCGCGC
TTCCGAGTGAACGCGTTCAACCAGAACCGCGGTGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAGGTGCTCACCCTCGA
GGACCTGGCCTGCCCGCCGCTGTTCCGCGAAGTGATCCAGCAGCCGCAGGGCCTGATCCTGGTAACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACATCAACAAGAACGAGTACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCATACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCATGGCTTCAATGA
AGCACTGCGCTCGGCGCTGCGTGAGGACCCGGACATCATTCTGGTGGGCGAACTTCGTGACCTGGAAACCATCCGCCTGG
CACTGACCGCAGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCATACCAGTTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAGTCGTTGCGCGCGGTCATTTCGCAGGC
GCTGCTGAAGAAGGTTGGCGGCGGTCGTACCGCAGCGTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCCATCCAGACTGGCCAGCAGTACGGCATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACCCGAAACCAGGCCCGTGAGTACGCCAAGGACAAGCGCCTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

78.488

99.71

0.783
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Vibrio cholerae strain A1552

39.943

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391