Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   POF65_RS04180 Genome accession   NZ_CP116706
Coordinates   757083..757868 (+) Length   261 a.a.
NCBI ID   WP_000825437.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain A909 sCas9     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 752083..762868
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF65_RS10860 - 753211..754005 (+) 795 WP_001873549.1 ABC transporter permease -
  POF65_RS04160 (POF65_04160) - 754106..754234 (+) 129 WP_000962057.1 hypothetical protein -
  POF65_RS04165 (POF65_04165) - 754243..754437 (+) 195 WP_000560510.1 hypothetical protein -
  POF65_RS04170 (POF65_04170) - 754649..755320 (+) 672 WP_000462434.1 ABC transporter ATP-binding protein -
  POF65_RS04175 (POF65_04175) - 755377..756822 (-) 1446 WP_000750939.1 FAD/NAD(P)-binding protein -
  POF65_RS04180 (POF65_04180) endA 757083..757868 (+) 786 WP_000825437.1 DNA/RNA non-specific endonuclease Machinery gene
  POF65_RS04185 (POF65_04185) - 757946..758584 (-) 639 WP_000478529.1 VTT domain-containing protein -
  POF65_RS04190 (POF65_04190) - 758801..759457 (+) 657 WP_000121094.1 ATP-binding cassette domain-containing protein -
  POF65_RS04195 (POF65_04195) fetB 759454..760227 (+) 774 WP_000011926.1 iron export ABC transporter permease subunit FetB -
  POF65_RS04200 (POF65_04200) - 760391..761209 (+) 819 WP_000600997.1 hypothetical protein -
  POF65_RS04205 (POF65_04205) - 761246..762130 (-) 885 WP_000354837.1 LysR family transcriptional regulator -

Sequence

Protein


Download         Length: 261 a.a.        Molecular weight: 29408.82 Da        Isoelectric Point: 8.9989

>NTDB_id=711023 POF65_RS04180 WP_000825437.1 757083..757868(+) (endA) [Streptococcus agalactiae strain A909 sCas9]
MKRLHKLFITVIATLGMLGVMTFGLPTQPQNVTPIVHADVNSSVDTSQEFQNNLKNAIGNLPFQYVNGIYELNNNQTNLN
ADVNVKAYVQNTIDNQQRPSTANAMLDRTIRQYQNRRDTTLPDANWKPLGWHQVATNDHYGHAVDKGHLIAYALAGNFKG
WDASVSNPQNVVTQTAHSNQSNQKINRGQNYYESLVRKAVDQNKRVRYRVTPLYRNDTDLVPFAMHLEAKSQDGTLEFNV
AIPNTQASYTMDYATGEITLN

Nucleotide


Download         Length: 786 bp        

>NTDB_id=711023 POF65_RS04180 WP_000825437.1 757083..757868(+) (endA) [Streptococcus agalactiae strain A909 sCas9]
ATGAAAAGATTACATAAACTGTTTATAACCGTAATTGCTACATTAGGTATGTTGGGGGTAATGACCTTTGGTCTTCCAAC
GCAGCCGCAAAACGTAACACCGATAGTACATGCTGATGTCAATTCATCTGTTGATACGAGCCAGGAATTTCAAAATAATT
TAAAAAATGCTATTGGTAACCTACCATTTCAATATGTTAATGGTATTTATGAATTAAATAATAATCAAACAAATTTAAAT
GCTGATGTCAATGTTAAAGCGTATGTTCAAAATACAATTGACAATCAACAAAGACCATCAACTGCTAATGCAATGCTTGA
TAGAACCATTCGTCAATATCAAAATCGCAGAGATACCACTCTTCCCGATGCAAATTGGAAACCATTAGGTTGGCATCAAG
TAGCTACTAATGACCATTATGGGCATGCAGTCGACAAGGGGCATTTAATTGCCTATGCTTTAGCTGGAAATTTCAAAGGT
TGGGATGCTTCCGTGTCAAATCCTCAAAATGTTGTCACACAAACAGCTCATTCCAACCAATCAAATCAAAAAATCAATCG
TGGACAAAATTATTATGAAAGCTTAGTTCGTAAGGCGGTTGACCAAAACAAACGTGTTCGTTACCGTGTAACTCCATTGT
ACCGTAATGATACTGATTTAGTTCCATTTGCAATGCACCTAGAAGCTAAATCACAAGATGGCACATTAGAATTTAATGTT
GCTATTCCAAACACACAAGCATCATACACTATGGATTATGCAACAGGAGAAATAACACTAAATTAA

Domains

Predicted by InterProScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(77-235)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

52.02

75.862

0.395
  endA Streptococcus pneumoniae D39

52.02

75.862

0.395
  endA Streptococcus pneumoniae R6

52.02

75.862

0.395
  endA Streptococcus pneumoniae TIGR4

52.02

75.862

0.395