Detailed information    

insolico Bioinformatically predicted

Overview

Name   mutS/mutS2   Type   Machinery gene
Locus tag   POF60_RS08425 Genome accession   NZ_CP116705
Coordinates   1637586..1639925 (-) Length   779 a.a.
NCBI ID   WP_001060329.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain CNCTC 10/84 dCas9     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1632586..1644925
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF60_RS08395 (POF60_08395) ssb 1632864..1633355 (-) 492 WP_000609586.1 single-stranded DNA-binding protein Machinery gene
  POF60_RS08400 (POF60_08400) rpsF 1633367..1633654 (-) 288 WP_001151773.1 30S ribosomal protein S6 -
  POF60_RS08405 (POF60_08405) mutY 1634717..1635871 (+) 1155 WP_162473108.1 A/G-specific adenine glycosylase -
  POF60_RS08410 (POF60_08410) - 1636048..1636641 (+) 594 WP_000402394.1 helix-turn-helix transcriptional regulator -
  POF60_RS08415 (POF60_08415) trxA 1636687..1637019 (-) 333 WP_001932060.1 thioredoxin -
  POF60_RS08420 (POF60_08420) - 1637082..1637582 (-) 501 WP_000446809.1 phosphatase PAP2 family protein -
  POF60_RS08425 (POF60_08425) mutS/mutS2 1637586..1639925 (-) 2340 WP_001060329.1 endonuclease MutS2 Machinery gene
  POF60_RS08430 (POF60_08430) - 1640010..1640552 (-) 543 WP_000949954.1 CvpA family protein -
  POF60_RS08435 (POF60_08435) zapA 1640555..1640866 (-) 312 WP_000448289.1 cell division protein ZapA -
  POF60_RS08440 (POF60_08440) rnhC 1640977..1641870 (+) 894 WP_001092531.1 ribonuclease HIII -
  POF60_RS08445 (POF60_08445) lepB 1641886..1642479 (+) 594 WP_000657514.1 signal peptidase I -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 87668.73 Da        Isoelectric Point: 6.3004

>NTDB_id=710959 POF60_RS08425 WP_001060329.1 1637586..1639925(-) (mutS/mutS2) [Streptococcus agalactiae strain CNCTC 10/84 dCas9]
MNNKILEQLEFNKVKELILPYLKTEQSQEELSGLEPMTEAPKIEKSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLEL
SADLNIQELLAIKKVLQSSSDMIHFYSDLDNVSFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTVYIEPRAVVTLNEEITQLRADER
HEESRILHAFSDLLRPHVATIRNNAWILGHLDFVRAKYLFMSDNKATIPEISNDSTLALINVRHPLLSNPVANDLHFDQD
LTAIVITGPNTGGKTIMLKTLGLAQLMGQSGLPVLADKGSKIAVFNNIFADIGDEQSIEQSLSTFSSHMTHIVSILNEAD
HNSLVLFDELGAGTDPQEGASLAMAILEHLRLSNIKTMATTHYPELKAYGIETNFVENASMEFDAETLSPTYRFMQGVPG
RSNAFEIASRLGLAPFIVKQAKQMTDSDSDVNRIIEQLEAQTLETRRRLDHIKEVEQENLKFNRAVKKLYNEFSHERDKE
LEKIYQEAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKKIKAARAPRIGDDIIVT
SYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFTLVRVQEEQKVKSKQINVVKKADSSGPRARLDLRGKRYEEAMQELDN
FIDQALLNNMGQVDIIHGIGTGVIREGVTKYLRRNKHVKHFAYAPQNAGGSGATIVTLG

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=710959 POF60_RS08425 WP_001060329.1 1637586..1639925(-) (mutS/mutS2) [Streptococcus agalactiae strain CNCTC 10/84 dCas9]
ATGAATAACAAGATTTTAGAACAGTTAGAATTTAACAAAGTTAAGGAATTGATATTACCTTATCTCAAGACAGAACAATC
ACAAGAAGAATTATCTGGGCTGGAGCCGATGACGGAGGCTCCTAAAATAGAAAAAAGTTTTAATGAAATTTCTGACATGG
AACAGATTTTTGTTGAACATCACTCATTTGGCATAGTCAGCCTAAGTTCAATCTCTGAGAGTTTAAAACGCTTAGAGCTT
TCAGCTGATCTTAATATTCAAGAACTTTTGGCTATCAAAAAAGTTTTACAGAGTTCTTCGGATATGATTCACTTTTATTC
TGATTTGGATAATGTTTCTTTCCAATCTTTGGATCGTTTGTTTGAAAATTTGGAACAATTCCCTAATCTGCAAGGGTCTT
TTCAAGCTATCAATGATGGTGGTTTTTTAGAACATTTTGCGAGTCCAGAATTAGAGCGTATCCGTCGTCAATTAACAAAC
AGTGAACGACGGGTTCGTCAGATTTTACAGGATATGCTTAAGGAAAAAGCAGAGCTTTTATCAGAGAATCTAATCGCTAG
TCGTAGTGGACGAAGTGTCCTACCAGTAAAAAATACTTATCGGAATCGTATTTCTGGTGTGGTTCATGACATCTCTTCTT
CAGGAAGTACTGTTTATATTGAGCCTCGTGCTGTAGTTACACTAAACGAAGAGATAACGCAGCTTAGAGCTGACGAACGT
CATGAAGAAAGTCGTATTTTACACGCATTTTCAGACTTGTTAAGACCCCATGTCGCCACTATTAGAAATAATGCATGGAT
TCTTGGGCATCTTGATTTTGTTAGGGCTAAATATCTTTTTATGTCTGATAATAAGGCGACGATACCTGAGATTTCTAATG
ACAGCACGTTAGCATTAATCAATGTTCGTCATCCTCTGTTAAGTAACCCTGTGGCTAATGACTTACATTTTGATCAAGAT
TTAACTGCAATTGTCATCACTGGTCCCAATACTGGTGGTAAGACGATTATGTTAAAAACACTCGGTTTAGCACAATTAAT
GGGACAGTCTGGTTTGCCAGTTTTAGCGGATAAAGGTAGTAAAATTGCAGTATTTAACAATATCTTTGCAGATATTGGCG
ATGAGCAATCTATTGAACAAAGTCTATCAACTTTTTCTAGTCATATGACGCACATAGTTAGTATTTTAAACGAGGCTGAC
CACAATAGTTTAGTTCTCTTTGATGAACTGGGAGCAGGAACGGATCCTCAAGAAGGTGCTAGTTTGGCTATGGCTATTTT
AGAACATCTTAGGTTAAGTAATATCAAAACGATGGCGACCACACACTATCCAGAATTAAAAGCTTATGGGATTGAGACAA
ATTTTGTAGAGAATGCGAGCATGGAATTTGATGCCGAAACGCTTAGCCCTACGTATCGCTTTATGCAAGGAGTTCCTGGA
CGATCAAATGCATTTGAAATTGCTTCTCGCCTTGGTTTAGCTCCATTTATTGTTAAACAAGCTAAGCAGATGACAGATTC
TGACTCAGATGTTAACCGTATTATTGAACAGTTAGAGGCACAGACACTTGAGACACGTAGAAGACTGGATCATATTAAAG
AAGTTGAACAAGAAAACCTCAAATTCAATCGTGCGGTTAAGAAACTCTATAATGAATTTTCACATGAGCGCGATAAAGAG
TTAGAAAAAATCTATCAAGAAGCTCAAGAAATTGTAGATATGGCTTTGAATGAGAGTGATACTATCTTAAAAAAACTCAA
TGATAAGAGCCAATTAAAACCTCACGAAATTATAGATGCTAAGGCACAAATAAAAAAATTAGCACCTCAAGTTGATTTAT
CAAAAAATAAAGTCTTAAATAAGGCTAAAAAAATCAAGGCAGCTCGTGCCCCTAGAATTGGTGATGATATTATAGTGACT
AGCTATGGACAGCGAGGTACCTTAACTAGTCAATTAAAAGATGGACGTTGGGAAGCACAAGTGGGAATTATCAAAATGAC
ATTAACACAAGATGAATTTACCCTCGTTAGAGTCCAAGAAGAACAGAAAGTCAAAAGTAAACAGATTAATGTGGTTAAAA
AGGCTGATAGTTCTGGACCAAGAGCTCGACTTGATCTTAGAGGTAAAAGATACGAAGAAGCTATGCAAGAGTTAGATAAT
TTTATTGATCAAGCATTGCTTAACAATATGGGACAAGTTGATATCATTCATGGTATTGGTACAGGCGTTATCCGTGAGGG
AGTGACAAAATACCTTCGTCGTAATAAGCACGTTAAACATTTTGCTTATGCCCCACAAAATGCAGGGGGATCTGGTGCCA
CAATTGTAACGTTAGGGTAG

Domains

Predicted by InterProScan.

MutS domain V (MutS_V)

(324-505)

Smr domain (Smr)

(704-778)

MutS2 and Smr-associated SH3 domain (MSSS)

(633-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  mutS/mutS2 Bacillus subtilis subsp. subtilis str. 168

40.38

100

0.41