Detailed information    

experimental Experimentally validated

Overview

Name   MMJJ_13030   Type   Unclear
Locus tag   MMJJ_RS06470 Genome accession   NZ_CP026606
Coordinates   1193565..1194761 (+) Length   398 a.a.
NCBI ID   WP_104838144.1    Uniprot ID   A0A2L1CBD2
Organism   Methanococcus maripaludis strain DSM 2067     
Function   require for natural transformation   
Unclear

Function

In-frame deletions of MMJJ_13020 and MMJJ_13030 in M. maripaludis resulted in complete transformation defects, which were rescued by complementation in trans.


Genomic Context

Location: 1188565..1199761
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMJJ_RS06430 (MMJJ_12950) - 1189139..1189729 (+) 591 WP_104838140.1 haloacid dehalogenase -
  MMJJ_RS06440 (MMJJ_12970) - 1190018..1190335 (+) 318 WP_013999856.1 DUF4870 domain-containing protein -
  MMJJ_RS06445 (MMJJ_12980) - 1190357..1191178 (-) 822 WP_104838141.1 NAD(P)H-hydrate dehydratase -
  MMJJ_RS06450 (MMJJ_12990) - 1191175..1191735 (-) 561 WP_104838142.1 DUF3343 domain-containing protein -
  MMJJ_RS06455 (MMJJ_13000) - 1191797..1192267 (-) 471 WP_011171482.1 YeeE/YedE thiosulfate transporter family protein -
  MMJJ_RS06460 (MMJJ_13010) - 1192290..1192745 (-) 456 WP_011171481.1 cytochrome c -
  MMJJ_RS06465 (MMJJ_13020) MMJJ_13020 1192953..1193564 (+) 612 WP_104838143.1 hypothetical protein Unclear
  MMJJ_RS06470 (MMJJ_13030) MMJJ_13030 1193565..1194761 (+) 1197 WP_104838144.1 tripartite tricarboxylate transporter permease Unclear
  MMJJ_RS06475 (MMJJ_13040) - 1194769..1195314 (-) 546 WP_104838145.1 adenylate kinase family protein -
  MMJJ_RS06480 (MMJJ_13050) - 1195323..1195775 (-) 453 WP_011171477.1 hypothetical protein -
  MMJJ_RS06485 (MMJJ_13060) - 1196142..1197386 (+) 1245 WP_013999848.1 phosphoglycerate kinase -
  MMJJ_RS06490 (MMJJ_13070) - 1197534..1198505 (+) 972 WP_104838146.1 methanogenesis marker 2 protein -
  MMJJ_RS06495 (MMJJ_13080) - 1198515..1198805 (+) 291 WP_104838147.1 hypothetical protein -
  MMJJ_RS06500 (MMJJ_13090) - 1198802..1199512 (+) 711 WP_104838148.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 398 a.a.        Molecular weight: 44653.40 Da        Isoelectric Point: 9.3225

>NTDB_id=71 MMJJ_RS06470 WP_104838144.1 1193565..1194761(+) (MMJJ_13030) [Methanococcus maripaludis strain DSM 2067]
MINNLFFLIFGTFTGTITGLIPGIHPNTLIPISIILYPYFGSSNYFSFLIGLLISHYFLNYIPSAFIGVPDDESAVAAVP
MHNLTILGRGYEAVVLAGFGAFFGIICSIFLFLIISRINIDFQSFYSYLKPFLPYILMLFLLISVIFSKNRVWTTLIILF
SGILGIIVFYKNPSFDYSLTVIFTGMFGIPLLFENLTKKELKSQFISFPELKLSYLKSAVFGTFGGFLRIFIPATGGAQI
NYFLSKLIKEENIENFIISQGSITLSNELFSILALMMIGTGRSGISEAIRTLKIEYTQSELFSSVLIATGISFLSLTVIS
KYFLQNINKFDYGLISKVLIVFCTILVLILSFKAYLIYHVVIYLISISIGVLCVKNRVNLSNMMGVLIFPTILYFLKI

Nucleotide


Download         Length: 1197 bp        

>NTDB_id=71 MMJJ_RS06470 WP_104838144.1 1193565..1194761(+) (MMJJ_13030) [Methanococcus maripaludis strain DSM 2067]
ATGATTAATAATTTATTCTTTTTAATTTTTGGAACATTTACTGGCACAATTACAGGATTAATCCCTGGAATTCATCCAAA
TACGCTTATTCCCATTTCTATAATTTTATATCCTTATTTTGGAAGCAGTAATTATTTTAGTTTTTTAATCGGGCTTTTAA
TTTCACACTATTTTTTAAATTACATTCCTTCCGCATTTATCGGGGTTCCTGACGACGAATCTGCAGTAGCAGCGGTTCCA
ATGCACAATTTAACGATTTTGGGAAGGGGCTACGAAGCAGTAGTTCTGGCAGGTTTTGGAGCATTTTTTGGAATAATCTG
TTCAATATTCCTATTTTTGATAATTTCCAGAATAAATATTGATTTTCAAAGTTTTTATTCATATTTAAAACCATTTCTTC
CATATATTTTGATGTTATTTCTTTTAATTTCAGTTATTTTTTCAAAAAATCGGGTCTGGACTACTTTAATAATTTTATTT
TCAGGAATTTTGGGAATTATTGTATTTTATAAAAATCCCAGTTTTGATTATTCTTTAACCGTGATATTTACAGGAATGTT
TGGAATTCCGCTTTTATTTGAAAATTTAACTAAAAAAGAACTCAAAAGCCAGTTTATCAGTTTTCCTGAACTAAAATTGT
CGTATCTAAAATCTGCAGTTTTTGGAACTTTTGGCGGATTTTTAAGGATATTTATTCCGGCAACCGGTGGCGCACAGATT
AATTATTTTTTAAGTAAATTGATAAAAGAAGAAAATATCGAAAATTTTATAATTTCACAAGGTTCAATAACTCTTTCAAA
CGAGCTTTTTTCCATTTTGGCACTCATGATGATTGGAACTGGAAGAAGCGGAATTTCAGAAGCTATAAGAACTTTAAAAA
TAGAATACACTCAGTCAGAACTTTTTTCAAGCGTACTTATTGCAACAGGTATTTCTTTTCTGAGTTTAACCGTGATTTCA
AAATATTTCTTACAAAACATTAATAAATTTGATTACGGGCTAATTTCAAAAGTTTTGATAGTTTTTTGCACGATTTTAGT
TTTAATTCTTTCTTTTAAAGCTTATTTGATTTATCACGTTGTAATATATTTAATATCAATCTCAATCGGAGTTTTATGTG
TTAAAAACAGGGTAAATCTATCAAACATGATGGGTGTATTAATATTCCCAACAATTCTTTATTTTTTAAAAATTTAA

Domains

Predicted by InterproScan.

Tripartite tricarboxylate transporter TctA family (TctA)

(5-384)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L1CBD2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Dallas R Fonseca et al. (2023) Random transposon mutagenesis identifies genes essential for transformation in Methanococcus maripaludis. Molecular Genetics And Genomics : MGG 298(3):537-548. [PMID: 36823423]