Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   NMJ60_RS08255 Genome accession   NZ_CP101314
Coordinates   1680692..1681762 (-) Length   356 a.a.
NCBI ID   WP_000776422.1    Uniprot ID   -
Organism   Staphylococcus aureus strain #492     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1675692..1686762
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMJ60_RS08215 gcvPA 1675869..1677215 (-) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  NMJ60_RS08220 gcvT 1677235..1678326 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  NMJ60_RS08225 - 1678485..1679009 (-) 525 WP_001015120.1 shikimate kinase -
  NMJ60_RS08230 - 1678999..1679145 (-) 147 WP_001792109.1 hypothetical protein -
  NMJ60_RS08235 comGF 1679242..1679739 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  NMJ60_RS08240 comGE 1679657..1679956 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  NMJ60_RS08245 comGD 1679943..1680389 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  NMJ60_RS08250 comGC 1680367..1680678 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  NMJ60_RS08255 comGB 1680692..1681762 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  NMJ60_RS08260 comGA 1681734..1682708 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  NMJ60_RS08265 - 1682760..1683383 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  NMJ60_RS08270 - 1683380..1683709 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  NMJ60_RS08275 - 1683709..1684695 (-) 987 WP_000161311.1 ROK family glucokinase -
  NMJ60_RS08280 - 1684692..1684895 (-) 204 WP_000087558.1 YqgQ family protein -
  NMJ60_RS08285 - 1684876..1686339 (-) 1464 WP_001017867.1 rhomboid family protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41447.84 Da        Isoelectric Point: 10.0606

>NTDB_id=709642 NMJ60_RS08255 WP_000776422.1 1680692..1681762(-) (comGB) [Staphylococcus aureus strain #492]
MKLQWINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=709642 NMJ60_RS08255 WP_000776422.1 1680692..1681762(-) (comGB) [Staphylococcus aureus strain #492]
GTGAAACTACAATGGATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGCCCAACAAATCGATTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGTACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAATCAAATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATTATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTTTCTATCTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAATTTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CAGGCTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTGCCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGGAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

100

100

1
  comGB Staphylococcus aureus N315

100

100

1