Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   PHA52_RS23645 Genome accession   NZ_CP116237
Coordinates   5101914..5102339 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain MPAO1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5096914..5107339
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PHA52_RS23630 (PHA52_23610) pilX 5097484..5098071 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  PHA52_RS23635 (PHA52_23615) pilY1 5098083..5101568 (+) 3486 WP_003115287.1 type 4a pilus biogenesis protein PilY1 -
  PHA52_RS23640 (PHA52_23620) pilY2 5101570..5101917 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  PHA52_RS23645 (PHA52_23625) comF 5101914..5102339 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  PHA52_RS23650 (PHA52_23630) ispH 5102386..5103330 (-) 945 WP_003112824.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  PHA52_RS23655 (PHA52_23635) fkpB 5103416..5103856 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  PHA52_RS23660 (PHA52_23640) lspA 5103849..5104358 (-) 510 WP_003112823.1 signal peptidase II -
  PHA52_RS23665 (PHA52_23645) ileS 5104351..5107182 (-) 2832 WP_003112822.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=709314 PHA52_RS23645 WP_003094721.1 5101914..5102339(+) (comF) [Pseudomonas aeruginosa strain MPAO1]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=709314 PHA52_RS23645 WP_003094721.1 5101914..5102339(+) (comF) [Pseudomonas aeruginosa strain MPAO1]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383