Detailed information    

insolico Bioinformatically predicted

Overview

Name   mutS/mutS2   Type   Machinery gene
Locus tag   PF976_RS13860 Genome accession   NZ_CP116011
Coordinates   2665552..2667909 (-) Length   785 a.a.
NCBI ID   WP_014472156.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain SRCM124317     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2660552..2672909
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF976_RS13835 (PF976_13835) - 2661051..2661830 (-) 780 WP_013353107.1 enoyl-CoA hydratase -
  PF976_RS13840 (PF976_13840) - 2661844..2662428 (-) 585 WP_014470798.1 TetR/AcrR family transcriptional regulator -
  PF976_RS13845 (PF976_13845) - 2662519..2664207 (-) 1689 WP_013353109.1 AMP-binding protein -
  PF976_RS13850 (PF976_13850) - 2664313..2664990 (+) 678 WP_014472154.1 DUF2711 family protein -
  PF976_RS13855 (PF976_13855) - 2665131..2665535 (-) 405 WP_003152541.1 DUF350 domain-containing protein -
  PF976_RS13860 (PF976_13860) mutS/mutS2 2665552..2667909 (-) 2358 WP_014472156.1 endonuclease MutS2 Machinery gene
  PF976_RS13865 (PF976_13865) polX 2667930..2669642 (-) 1713 WP_038463272.1 DNA polymerase/3'-5' exonuclease PolX -
  PF976_RS13870 (PF976_13870) - 2669748..2670281 (-) 534 WP_013353113.1 CvpA family protein -
  PF976_RS13875 (PF976_13875) zapA 2670289..2670546 (-) 258 WP_003152533.1 cell division protein ZapA -
  PF976_RS13880 (PF976_13880) rnhC 2670680..2671621 (+) 942 WP_013353114.1 ribonuclease HIII -

Sequence

Protein


Download         Length: 785 a.a.        Molecular weight: 87412.75 Da        Isoelectric Point: 6.3218

>NTDB_id=706788 PF976_RS13860 WP_014472156.1 2665552..2667909(-) (mutS/mutS2) [Bacillus amyloliquefaciens strain SRCM124317]
MQQKVLSSLEFHKVKEQITAHAASSLGREKLLQLKPLTDLADIQKQLDEVEEASAIMRLRGHAPFGGLTDIRSALRRAEI
GSVLTPAEFTELSGLLYAVKQMKHFISQMTEDGVSIPLIQAHAEELITLGDLEREINSCIDDHGEVLDHASPALRGIRTQ
LRTLESRVRDRLESMLRSSSASKMLSDTIVTIRNDRFVIPVKQEYRSSYGGIVHDTSSSGATLFIEPQAIVDMNNSLQQA
KVKEKQEIERILRMLTEHTAEHTQEIVQNVEVLQTLDFIFAKARYAKAMKATKPFMNGDGFIRLKKARHPLLPQDQVVAN
DIELGGDYTTIVITGPNTGGKTVTLKTLGLLTIMAQAGLHIPADEGSEAAVFDHVFADIGDEQSIEQSLSTFSSHMVNIV
SILKDVSENSLVLFDELGAGTDPQEGAALAMSILDEVHRTNARVLATTHYPELKAYGYNRQGVMNASVEFDIETLSPTYK
LLIGVPGRSNAFEISRRLGLPEHIIGQAKSEMTAEHNEVDLMIASLEQSKKRADEELSETESIRKEAEKLHKELQQQIIE
LNAQKDKLMEEAEQKAAEKLEDAAKEAEQIIRELRSIKQEHRSFKEHELIDAKKRLGDAMPAFEKSKQPERKTEKKRELK
PGDEVKVLTFGQKGALLEKTGEKEWNVQIGILKMKVKEKDLEFLKSAPEPKKEKAITAVKGKDYHVSLELDLRGERYENA
LSRVEKYLDDAVLAGYPRVSIIHGKGTGALRKGVQDLLKNHRSVKSSRFGEAGEGGSGVTIVELK

Nucleotide


Download         Length: 2358 bp        

>NTDB_id=706788 PF976_RS13860 WP_014472156.1 2665552..2667909(-) (mutS/mutS2) [Bacillus amyloliquefaciens strain SRCM124317]
GTGCAGCAAAAAGTGTTATCATCACTTGAATTTCATAAAGTAAAAGAACAGATTACCGCACATGCCGCCTCATCGCTCGG
CCGGGAAAAGCTTCTGCAGCTGAAGCCTTTGACTGATCTCGCCGACATCCAAAAACAGCTGGATGAAGTCGAAGAAGCAT
CCGCGATCATGCGCTTAAGAGGCCATGCTCCGTTCGGCGGTCTGACCGATATAAGATCGGCTTTGCGTCGGGCGGAAATC
GGCAGCGTGCTGACCCCGGCGGAATTCACCGAATTGTCCGGGCTGCTGTATGCCGTTAAGCAAATGAAGCACTTTATCAG
TCAAATGACAGAAGACGGCGTCAGCATTCCGCTGATCCAGGCCCATGCAGAAGAGCTGATTACATTGGGCGATTTGGAAA
GGGAGATTAATTCCTGCATTGATGATCACGGCGAAGTGCTTGATCATGCATCTCCCGCTTTAAGAGGAATCCGCACGCAG
CTCAGAACGCTTGAATCAAGAGTGAGAGACCGGCTTGAATCGATGCTGCGTTCATCTTCCGCTTCAAAAATGCTGTCTGA
CACGATCGTTACGATTCGGAATGACCGCTTTGTCATTCCGGTCAAACAGGAATACAGATCAAGCTACGGCGGGATCGTGC
ATGATACTTCTTCATCAGGTGCGACGCTGTTTATTGAACCGCAGGCGATCGTCGATATGAACAACTCGCTTCAGCAGGCG
AAGGTGAAAGAAAAACAGGAAATCGAACGGATTCTCCGGATGCTGACGGAGCATACGGCTGAACACACACAAGAGATTGT
TCAAAATGTGGAAGTGCTGCAGACGCTGGATTTCATTTTCGCAAAAGCGAGATACGCAAAAGCGATGAAGGCGACAAAAC
CGTTTATGAACGGAGACGGCTTTATCCGTCTGAAAAAAGCGCGCCATCCGCTGCTGCCTCAAGATCAAGTCGTGGCCAAC
GACATTGAACTGGGCGGAGATTATACGACGATCGTTATCACGGGTCCGAATACGGGCGGTAAAACCGTTACGTTAAAAAC
GCTCGGGCTGCTGACGATAATGGCGCAGGCAGGACTGCACATACCGGCTGATGAAGGATCGGAAGCCGCCGTATTTGATC
ATGTTTTTGCCGATATCGGTGATGAACAGTCGATCGAACAAAGCCTGAGTACGTTCTCATCTCACATGGTCAATATCGTC
AGTATTTTAAAAGACGTGTCCGAAAACAGTCTTGTGCTGTTTGATGAACTCGGTGCCGGAACCGACCCGCAGGAAGGGGC
GGCGCTTGCCATGAGCATTCTTGACGAAGTCCACCGGACAAACGCCAGAGTGCTTGCGACAACCCATTATCCGGAGCTGA
AAGCATACGGATATAACAGACAAGGCGTCATGAATGCAAGCGTTGAATTTGATATTGAAACGCTTTCGCCGACCTACAAG
CTCCTGATCGGAGTGCCGGGCCGAAGCAACGCTTTTGAAATCTCAAGACGCCTCGGCCTGCCGGAGCATATCATCGGCCA
GGCGAAGTCAGAAATGACCGCCGAACACAATGAAGTCGATCTGATGATCGCGTCACTTGAACAAAGTAAAAAAAGAGCGG
ATGAAGAGCTTTCTGAAACCGAATCAATCAGAAAAGAAGCAGAAAAACTGCATAAAGAGCTGCAGCAGCAAATCATTGAG
CTGAACGCCCAGAAAGACAAACTGATGGAAGAAGCCGAACAAAAAGCAGCGGAAAAACTGGAAGACGCCGCAAAAGAAGC
AGAACAGATCATTCGCGAGCTCCGGTCTATCAAGCAAGAACACAGATCCTTTAAGGAACACGAGCTGATTGATGCCAAAA
AACGTCTCGGAGACGCGATGCCGGCTTTTGAAAAATCAAAGCAGCCGGAAAGAAAAACAGAGAAAAAACGCGAGCTGAAG
CCGGGTGACGAAGTAAAGGTGCTCACATTCGGACAAAAAGGGGCGCTGCTTGAAAAAACCGGCGAGAAAGAATGGAATGT
CCAAATCGGCATCCTTAAAATGAAGGTGAAGGAAAAAGACCTTGAGTTTCTCAAGTCCGCTCCTGAACCGAAAAAAGAAA
AAGCCATCACCGCCGTAAAAGGGAAGGATTATCACGTGTCCCTTGAACTTGACCTGCGGGGAGAGCGTTACGAAAATGCC
CTCAGCCGCGTCGAAAAATATTTAGATGACGCGGTGCTTGCCGGCTATCCGAGAGTGTCCATCATTCACGGAAAAGGCAC
AGGCGCCCTCCGAAAAGGGGTGCAGGATCTTCTGAAAAACCACCGCAGCGTGAAAAGCTCGCGGTTCGGGGAAGCGGGAG
AAGGAGGATCAGGCGTAACGATTGTCGAACTAAAATAA

Domains

Predicted by InterProScan.

MutS2 and Smr-associated SH3 domain (MSSS)

(641-681)

MutS domain V (MutS_V)

(331-512)

Smr domain (Smr)

(710-785)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  mutS/mutS2 Bacillus subtilis subsp. subtilis str. 168

87.771

100

0.878