Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   PCS83_RS21990 Genome accession   NZ_CP115320
Coordinates   4468735..4469499 (+) Length   254 a.a.
NCBI ID   WP_001136232.1    Uniprot ID   Q0TBX9
Organism   Escherichia coli strain CUVET18-565     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4463735..4474499
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCS83_RS21970 (PCS83_21910) acpT 4464689..4465276 (+) 588 WP_000285790.1 4'-phosphopantetheinyl transferase AcpT -
  PCS83_RS21975 (PCS83_21915) nikA 4465387..4466961 (+) 1575 WP_000493122.1 nickel ABC transporter substrate-binding protein -
  PCS83_RS21980 (PCS83_21920) nikB 4466961..4467905 (+) 945 WP_000947070.1 nickel ABC transporter permease subunit NikB -
  PCS83_RS21985 (PCS83_21925) nikC 4467902..4468735 (+) 834 WP_001008955.1 nickel ABC transporter permease subunit NikC -
  PCS83_RS21990 (PCS83_21930) amiE 4468735..4469499 (+) 765 WP_001136232.1 nickel import ATP-binding protein NikD Regulator
  PCS83_RS21995 (PCS83_21935) nikE 4469496..4470302 (+) 807 WP_000173679.1 nickel import ATP-binding protein NikE -
  PCS83_RS22000 (PCS83_21940) nikR 4470308..4470709 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  PCS83_RS22005 (PCS83_21945) yhhJ 4470718..4471842 (-) 1125 WP_001314210.1 ABC transporter permease -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26812.40 Da        Isoelectric Point: 6.6882

>NTDB_id=703225 PCS83_RS21990 WP_001136232.1 4468735..4469499(+) (amiE) [Escherichia coli strain CUVET18-565]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=703225 PCS83_RS21990 WP_001136232.1 4468735..4469499(+) (amiE) [Escherichia coli strain CUVET18-565]
ATGCCACAACAGATTGAACTCCGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGCA
ACGTGGGCGTGTGCTGGCGTTAGTCGGCGGTAGTGGCAGCGGGAAGTCGCTGACCTGCGCCGCGACGCTGGGCATTCTGC
CTGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTGGCCGATGGAAAACCGGTTTCTCCCTGCGCCCTGCGCGGAATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGTGCCTTTAATCCGCTGCACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGATCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTTACCCATGATATGGGCGTGGTAGCGCGTCTGGCAGACGATGTGGCGGTAA
TGTCTCACGGTAAGATTGTTGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0TBX9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398