Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   PCP64_RS04135 Genome accession   NZ_CP115276
Coordinates   847835..848260 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain F013     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 842835..853260
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP64_RS04120 (PCP64_04115) pilX 843399..843986 (+) 588 WP_023090846.1 type 4a pilus minor pilin PilX -
  PCP64_RS04125 (PCP64_04120) pilY1 843998..847489 (+) 3492 WP_003123397.1 type 4a pilus biogenesis protein PilY1 -
  PCP64_RS04130 (PCP64_04125) pilY2 847491..847838 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  PCP64_RS04135 (PCP64_04130) comF 847835..848260 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  PCP64_RS04140 (PCP64_04135) ispH 848307..849251 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  PCP64_RS04145 (PCP64_04140) fkpB 849337..849777 (-) 441 WP_003161775.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  PCP64_RS04150 (PCP64_04145) lspA 849770..850279 (-) 510 WP_003102615.1 signal peptidase II -
  PCP64_RS04155 (PCP64_04150) ileS 850272..853103 (-) 2832 WP_003123398.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=701251 PCP64_RS04135 WP_003094721.1 847835..848260(+) (comF) [Pseudomonas aeruginosa strain F013]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=701251 PCP64_RS04135 WP_003094721.1 847835..848260(+) (comF) [Pseudomonas aeruginosa strain F013]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383