Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NG745_RS01795 Genome accession   NZ_CP099465
Coordinates   338727..339164 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain Ag75     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 333727..344164
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NG745_RS01770 (NG745_01770) - 334747..335883 (+) 1137 WP_007609290.1 zinc-dependent alcohol dehydrogenase -
  NG745_RS01775 (NG745_01775) - 335898..336332 (+) 435 WP_063096155.1 RDD family protein -
  NG745_RS01780 (NG745_01780) - 336404..336727 (+) 324 WP_052827031.1 YckD family protein -
  NG745_RS01785 (NG745_01785) - 336831..338267 (+) 1437 WP_098080961.1 family 1 glycosylhydrolase -
  NG745_RS01790 (NG745_01790) nin/comJ 338308..338706 (-) 399 WP_020955304.1 competence protein ComJ Regulator
  NG745_RS01795 (NG745_01795) nucA/comI 338727..339164 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NG745_RS01800 (NG745_01800) hxlB 339524..340081 (-) 558 WP_007409354.1 6-phospho-3-hexuloisomerase -
  NG745_RS01805 (NG745_01805) hxlA 340078..340713 (-) 636 WP_025284209.1 3-hexulose-6-phosphate synthase -
  NG745_RS01810 (NG745_01810) - 340945..341307 (+) 363 WP_007409352.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=699841 NG745_RS01795 WP_003156588.1 338727..339164(-) (nucA/comI) [Bacillus velezensis strain Ag75]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=699841 NG745_RS01795 WP_003156588.1 338727..339164(-) (nucA/comI) [Bacillus velezensis strain Ag75]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCCGTCAAAACGGGATACGATAGAGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641