Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   PCP14_RS05025 Genome accession   NZ_CP115251
Coordinates   1058849..1059274 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain F035     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1053849..1064274
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP14_RS05010 (PCP14_05000) pilX 1054413..1055000 (+) 588 WP_015648054.1 type 4a pilus minor pilin PilX -
  PCP14_RS05015 (PCP14_05005) pilY1 1055012..1058503 (+) 3492 WP_003123397.1 type 4a pilus biogenesis protein PilY1 -
  PCP14_RS05020 (PCP14_05010) pilY2 1058505..1058852 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  PCP14_RS05025 (PCP14_05015) comF 1058849..1059274 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  PCP14_RS05030 (PCP14_05020) ispH 1059321..1060265 (-) 945 WP_003134939.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  PCP14_RS05035 (PCP14_05025) fkpB 1060351..1060791 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  PCP14_RS05040 (PCP14_05030) lspA 1060784..1061293 (-) 510 WP_003094728.1 signal peptidase II -
  PCP14_RS05045 (PCP14_05035) ileS 1061286..1064117 (-) 2832 WP_003094730.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=699008 PCP14_RS05025 WP_003094721.1 1058849..1059274(+) (comF) [Pseudomonas aeruginosa strain F035]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=699008 PCP14_RS05025 WP_003094721.1 1058849..1059274(+) (comF) [Pseudomonas aeruginosa strain F035]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACACCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACGCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383