Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   PCP22_RS30020 Genome accession   NZ_CP115246
Coordinates   6466163..6466588 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain F040     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6461163..6471588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP22_RS30005 (PCP22_29990) pilX 6461727..6462314 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  PCP22_RS30010 (PCP22_29995) pilY1 6462326..6465817 (+) 3492 WP_010793785.1 type 4a pilus biogenesis protein PilY1 -
  PCP22_RS30015 (PCP22_30000) pilY2 6465819..6466166 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  PCP22_RS30020 (PCP22_30005) comF 6466163..6466588 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  PCP22_RS30025 (PCP22_30010) ispH 6466635..6467579 (-) 945 WP_003112824.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  PCP22_RS30030 (PCP22_30015) fkpB 6467665..6468105 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  PCP22_RS30035 (PCP22_30020) lspA 6468098..6468607 (-) 510 WP_003102615.1 signal peptidase II -
  PCP22_RS30040 (PCP22_30025) ileS 6468600..6471431 (-) 2832 WP_003102617.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=698571 PCP22_RS30020 WP_003094721.1 6466163..6466588(+) (comF) [Pseudomonas aeruginosa strain F040]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=698571 PCP22_RS30020 WP_003094721.1 6466163..6466588(+) (comF) [Pseudomonas aeruginosa strain F040]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383