Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   PCP65_RS04360 Genome accession   NZ_CP115203
Coordinates   954790..955215 (-) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain F070     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 949790..960215
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP65_RS04340 (PCP65_04345) ileS 949947..952778 (+) 2832 WP_003119417.1 isoleucine--tRNA ligase -
  PCP65_RS04345 (PCP65_04350) lspA 952771..953280 (+) 510 WP_003102615.1 signal peptidase II -
  PCP65_RS04350 (PCP65_04355) fkpB 953273..953713 (+) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  PCP65_RS04355 (PCP65_04360) ispH 953799..954743 (+) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  PCP65_RS04360 (PCP65_04365) comF 954790..955215 (-) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  PCP65_RS04365 (PCP65_04370) pilY2 955212..955559 (-) 348 WP_003094713.1 type 4a fimbrial biogenesis protein PilY2 -
  PCP65_RS04370 (PCP65_04375) pilY1 955561..959046 (-) 3486 WP_124156819.1 type 4a pilus biogenesis protein PilY1 -
  PCP65_RS04375 (PCP65_04380) pilX 959058..959645 (-) 588 WP_030048355.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=695396 PCP65_RS04360 WP_003094721.1 954790..955215(-) (comF) [Pseudomonas aeruginosa strain F070]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=695396 PCP65_RS04360 WP_003094721.1 954790..955215(-) (comF) [Pseudomonas aeruginosa strain F070]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGGCAGGCGCTGCTCTCGGACGCGGCCGCGCGTC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383