Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   MDRSPN_RS09220 Genome accession   NZ_AP018391
Coordinates   1750486..1751310 (-) Length   274 a.a.
NCBI ID   WP_001036785.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain MDRSPN001     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1745486..1756310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MDRSPN_RS09215 (MDRSPN_01750) - 1748864..1750195 (-) 1332 WP_000390039.1 hemolysin family protein -
  MDRSPN_RS09220 (MDRSPN_01751) endA 1750486..1751310 (-) 825 WP_001036785.1 DNA-entry nuclease EndA Machinery gene
  MDRSPN_RS09225 (MDRSPN_01752) - 1751349..1751537 (-) 189 WP_001036519.1 DNA-directed RNA polymerase subunit beta -
  MDRSPN_RS09230 (MDRSPN_01753) murA 1751530..1752813 (-) 1284 WP_000358028.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  MDRSPN_RS09235 (MDRSPN_01754) - 1752837..1753067 (-) 231 WP_000250392.1 DUF1146 family protein -
  MDRSPN_RS09240 (MDRSPN_01755) sepM 1753125..1754162 (-) 1038 WP_000727277.1 SepM family pheromone-processing serine protease Regulator
  MDRSPN_RS09245 (MDRSPN_01756) coaD 1754146..1754634 (-) 489 WP_001280731.1 pantetheine-phosphate adenylyltransferase -
  MDRSPN_RS09250 (MDRSPN_01757) rsmD 1754624..1755163 (-) 540 WP_000706963.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  MDRSPN_RS09255 (MDRSPN_01758) asnA 1755228..1756220 (-) 993 WP_000747993.1 aspartate--ammonia ligase -

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29917.56 Da        Isoelectric Point: 10.0650

>NTDB_id=69340 MDRSPN_RS09220 WP_001036785.1 1750486..1751310(-) (endA) [Streptococcus pneumoniae strain MDRSPN001]
MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQAPEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSNATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=69340 MDRSPN_RS09220 WP_001036785.1 1750486..1751310(-) (endA) [Streptococcus pneumoniae strain MDRSPN001]
ATGAACAAAAAAACAAGACAGACACTAATCGGACTGCTAGTGTTATTGCTTTTGTCTACAGGGAGCTATTATATCAAGCA
GATGCAGTCGGCACCTAATAGTCCCAAAACCAATCTTAGTCAGAAAAAACAAGCGCCTGAAGCTCCTAGTCAAGCATTGG
CAGAGAGTGTCTTAACAGACGCAGTCAAGAGTCAAATAAAGGGGAGTCTGGAGTGGAATGGCTCAGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAGCCCTACGCTGACAATAAAACAAAGACAGTGGGCAAGGA
AACTGTTCCAACCGTAGCTAATGCCCTCTTGTCTAATGCCACTCGTCAGTACAAGAATCGTAAAGAAACTGGGAATGGTT
CAACTTCTTGGACTCCTCCAGGTTGGCATCAGGTCAAGAATCTAAAGGGCTCTTATACCCATGCAGTCGATAGAGGTCAT
TTGTTAGGCTATGCCTTAATCGGTGGTTTGGATGGTTTTGATGCCTCAACAAGCAATCCTAAAAACATTGCTGTTCAGAC
AGCCTGGGCAAATCAGGCACAAGCCGAGTATTCGACTGGTCAAAACTACTATGAAAGCAAGGTGCGTAAAGCCTTGGACC
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTACTACGCTTCAAACGAGGATTTAGTTCCCTCAGCTTCACAGATTGAA
GCCAAGTCTTCGGATGGAGAATTGGAATTCAATGTTCTAGTTCCCAATGTTCAAAAGGGACTTCAACTGGATTACCGAAC
TGGAGAAGTAACTGTAACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

98.905

100

0.989
  endA Streptococcus pneumoniae D39

98.905

100

0.989
  endA Streptococcus pneumoniae R6

98.905

100

0.989
  endA Streptococcus pneumoniae TIGR4

98.905

100

0.989

Multiple sequence alignment