Detailed information    

insolico Bioinformatically predicted

Overview

Name   mutS/mutS2   Type   Machinery gene
Locus tag   ORP33_RS14350 Genome accession   NZ_CP113256
Coordinates   2763891..2766248 (-) Length   785 a.a.
NCBI ID   WP_021480271.1    Uniprot ID   -
Organism   Bacillus subtilis strain K1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2758891..2771248
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ORP33_RS14325 (ORP33_13995) etfB 2759332..2760105 (-) 774 WP_004398765.1 electron transfer flavoprotein subunit beta -
  ORP33_RS14330 (ORP33_14000) fadB 2760120..2760896 (-) 777 WP_003229549.1 enoyl-CoA hydratase -
  ORP33_RS14335 (ORP33_14005) fadR 2760911..2761495 (-) 585 WP_003229547.1 fatty acid metabolism transcriptional regulator FadR -
  ORP33_RS14340 (ORP33_14010) lcfA 2761600..2763282 (-) 1683 WP_080529640.1 long-chain-fatty-acid--CoA ligase LcfA -
  ORP33_RS14345 (ORP33_14015) yshE 2763472..2763876 (-) 405 WP_003237674.1 DUF350 domain-containing protein -
  ORP33_RS14350 (ORP33_14020) mutS/mutS2 2763891..2766248 (-) 2358 WP_021480271.1 endonuclease MutS2 Machinery gene
  ORP33_RS14355 (ORP33_14025) polX 2766269..2767981 (-) 1713 WP_003229538.1 DNA polymerase/3'-5' exonuclease PolX -
  ORP33_RS14360 (ORP33_14030) yshB 2768055..2768588 (-) 534 WP_003229537.1 CvpA family protein -
  ORP33_RS14365 (ORP33_14035) zapA 2768595..2768852 (-) 258 WP_003229534.1 cell division protein ZapA -
  ORP33_RS14370 (ORP33_14040) rnhC 2768986..2769927 (+) 942 WP_024571958.1 ribonuclease HIII -

Sequence

Protein


Download         Length: 785 a.a.        Molecular weight: 87354.72 Da        Isoelectric Point: 6.1361

>NTDB_id=690441 ORP33_RS14350 WP_021480271.1 2763891..2766248(-) (mutS/mutS2) [Bacillus subtilis strain K1]
MQQKVLSALEFHKVKEQVIGHAASSLGKEMLLELKPSASIDEIKKQLDEVDEASDIIRLRGQAPFGGLVDIRGALRRAEI
GSVLSPSEFTEISGLLYAVKQMKHFITQMAEDGVDIPLIHQHAEQLITLSDLERDINSCIDDHGEVLDHASETLRGIRTQ
LRTLESRVRDRLESMLRSSSASKMLSDTIVTIRNDRFVIPVKQEYRSSYGGIVHDTSSSGATLFIEPQAIVDMNNSLQQA
KVKEKQEIERILRVLTEKTAEYTEELFLDLQVLQTLDFIFAKARYAKAVKATKPIMNDTGFIRLKKARHPLLPPDQVVAN
DIELGRDFSTIVITGPNTGGKTVTLKTLGLLTLMAQSGLHIPADEGSEAAVFEHVFADIGDEQSIEQSLSTFSSHMVNIV
GILEQVNENSLVLFDELGAGTDPQEGAALAMSILDDVHRTNARVLATTHYPELKAYGYNREGVMNASVEFDIETLSPTYK
LLIGVPGRSNAFEISKRLGLPDHIIGQAKSEMTAEHNEVDTMIASLEQSKKRAEEELSETESIRKEAEKLHKELQQQIIE
LNSKKDKMLEEAEQQAAEKVKAAMKEAEDIIHELRSIKEEHKSFKDHELINAKKRLEGAMPAFEKSKKPEKPKAQKRDFK
PGDEVKVLTFGQKGTLLEKTGGNEWNVQIGILKMKVKEKDLEFIKSAPEPKKEKIITAVKGKDYHVSLELDLRGERYENA
LSRVEKYLDDAVLAGYPRVSIIHGKGTGALRKGVQDLLKNHRSVKSSRFGEAGEGGSGVTVVELK

Nucleotide


Download         Length: 2358 bp        

>NTDB_id=690441 ORP33_RS14350 WP_021480271.1 2763891..2766248(-) (mutS/mutS2) [Bacillus subtilis strain K1]
GTGCAGCAAAAAGTATTATCAGCTCTTGAATTTCATAAAGTGAAAGAACAGGTTATTGGGCATGCCGCTTCATCGCTCGG
AAAAGAAATGCTTCTCGAGCTTAAGCCTTCTGCTTCTATAGACGAAATCAAAAAACAGCTGGATGAAGTAGACGAAGCTT
CTGACATTATCCGGCTGAGAGGCCAAGCGCCATTTGGCGGCCTTGTAGATATCAGAGGAGCGTTAAGACGGGCGGAAATC
GGCAGTGTTCTCAGTCCTTCTGAATTCACTGAAATCTCAGGCCTGCTTTATGCAGTTAAACAAATGAAACATTTTATCAC
CCAAATGGCTGAAGACGGTGTCGACATTCCGCTGATCCATCAGCATGCTGAACAGCTTATCACGCTGTCCGATTTAGAGC
GGGACATTAATTCCTGCATTGATGATCACGGAGAAGTGCTTGATCATGCATCGGAAACATTAAGAGGAATCCGCACACAG
CTCAGAACACTCGAATCAAGAGTCAGAGACCGGTTAGAGTCGATGCTGCGTTCCTCTTCCGCATCGAAAATGCTGTCTGA
TACGATTGTTACGATTCGGAATGACCGCTTTGTGATCCCGGTCAAACAGGAGTACAGATCCAGCTATGGAGGAATTGTGC
ACGACACCTCATCCTCTGGTGCGACACTATTCATTGAACCGCAGGCGATTGTAGATATGAACAATTCCCTTCAGCAGGCG
AAAGTGAAAGAAAAGCAAGAAATTGAACGGATTTTGCGTGTGCTGACAGAGAAAACGGCAGAGTATACAGAGGAACTATT
TCTAGATTTGCAAGTGCTGCAGACGCTTGACTTTATTTTTGCAAAAGCTAGATATGCAAAAGCGGTTAAAGCGACAAAAC
CGATTATGAACGACACCGGCTTTATCCGTTTGAAAAAAGCCCGCCATCCATTGCTTCCGCCTGATCAAGTTGTTGCCAAT
GACATCGAGCTTGGCCGCGATTTTTCAACCATTGTCATCACAGGGCCAAACACCGGGGGGAAAACAGTCACCCTTAAAAC
GTTAGGCCTGCTAACCTTAATGGCGCAGTCAGGTCTTCATATCCCGGCAGATGAAGGGTCAGAAGCGGCAGTATTTGAGC
ACGTATTCGCTGATATCGGTGATGAACAGTCGATTGAGCAAAGTTTAAGTACGTTCTCATCCCATATGGTGAATATTGTC
GGCATTTTAGAACAGGTCAATGAAAACAGCCTTGTGCTTTTCGATGAACTTGGTGCAGGGACAGATCCGCAGGAGGGGGC
GGCCCTCGCCATGAGCATCTTGGATGACGTGCATCGCACCAATGCACGAGTGTTAGCTACGACGCATTATCCGGAATTGA
AGGCGTACGGCTATAACAGAGAAGGCGTCATGAATGCCAGCGTTGAATTTGACATCGAAACGCTGTCACCGACCTATAAA
CTTTTAATTGGTGTGCCGGGTCGAAGCAATGCTTTCGAAATTTCAAAACGCCTCGGGCTTCCGGACCATATCATCGGGCA
GGCGAAGTCAGAAATGACGGCCGAGCATAACGAAGTCGATACGATGATTGCGTCGCTGGAACAAAGCAAAAAACGTGCGG
AAGAAGAGCTTTCTGAGACAGAATCAATCAGAAAAGAAGCGGAAAAACTGCATAAAGAGCTGCAGCAGCAAATCATCGAG
CTTAACAGCAAAAAAGACAAAATGCTTGAAGAGGCAGAACAGCAGGCTGCTGAAAAAGTAAAAGCGGCAATGAAAGAAGC
CGAGGACATTATTCATGAATTGCGCTCCATAAAAGAAGAACACAAATCCTTCAAGGATCACGAGCTGATTAACGCGAAGA
AACGGTTAGAAGGCGCTATGCCGGCTTTTGAAAAGTCCAAGAAACCGGAGAAGCCGAAAGCGCAAAAACGCGACTTTAAG
CCTGGCGACGAGGTGAAAGTCCTCACTTTCGGGCAAAAAGGAACATTGCTCGAAAAAACAGGCGGCAATGAATGGAATGT
CCAAATCGGTATTTTAAAGATGAAAGTAAAAGAAAAAGATCTGGAGTTTATCAAATCAGCTCCGGAGCCAAAAAAAGAAA
AAATCATTACAGCGGTCAAGGGAAAGGACTATCACGTATCGCTTGAACTTGATCTCCGCGGCGAACGCTATGAAAATGCC
CTAAGCCGGGTTGAAAAATACTTGGATGATGCGGTGTTAGCCGGATATCCAAGAGTGTCAATCATCCACGGAAAAGGAAC
CGGCGCTTTAAGAAAAGGCGTACAGGATCTTCTGAAAAACCACCGCAGCGTCAAAAGTTCCCGTTTCGGTGAAGCAGGTG
AGGGAGGATCAGGCGTTACGGTTGTTGAACTAAAATAA

Domains

Predicted by InterProScan.

MutS domain V (MutS_V)

(331-512)

Smr domain (Smr)

(710-785)

MutS2 and Smr-associated SH3 domain (MSSS)

(641-681)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  mutS/mutS2 Bacillus subtilis subsp. subtilis str. 168

99.618

100

0.996