Detailed information    

experimental Experimentally validated

Overview

Name   MMJJ_17200   Type   Unclear
Locus tag   MMJJ_RS08540 Genome accession   NZ_CP026606
Coordinates   1571750..1572223 (-) Length   157 a.a.
NCBI ID   WP_104838451.1    Uniprot ID   A0A2L1CCK8
Organism   Methanococcus maripaludis strain DSM 2067     
Function   require for natural transformation   
Unclear

Function

In-frame deletion mutants for MMJJ_17200 were completely defective for transformation, and complementation of the mutation in trans rescued this defect.


Genomic Context

Location: 1566750..1577223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMJJ_RS08515 (MMJJ_17150) - 1567020..1567418 (+) 399 WP_104838447.1 secondary thiamine-phosphate synthase enzyme YjbQ -
  MMJJ_RS08520 (MMJJ_17160) - 1567533..1568021 (+) 489 WP_013999554.1 peptidylprolyl isomerase -
  MMJJ_RS08525 (MMJJ_17170) - 1568063..1568665 (+) 603 WP_104838448.1 peptidylprolyl isomerase -
  MMJJ_RS08530 (MMJJ_17180) - 1568708..1570849 (-) 2142 WP_104838449.1 Tex family protein -
  MMJJ_RS08535 (MMJJ_17190) - 1570900..1571697 (-) 798 WP_104838450.1 NOL1/NOP2/sun family putative RNA methylase -
  MMJJ_RS08540 (MMJJ_17200) MMJJ_17200 1571750..1572223 (-) 474 WP_104838451.1 archaeosortase family protein ArtE Unclear
  MMJJ_RS08545 (MMJJ_17210) thiL 1572266..1573213 (-) 948 WP_104838596.1 thiamine-phosphate kinase -
  MMJJ_RS08550 (MMJJ_17220) - 1573556..1574872 (+) 1317 WP_104838452.1 B12-binding domain-containing radical SAM protein -
  MMJJ_RS08555 (MMJJ_17230) - 1574916..1576094 (+) 1179 WP_104838453.1 redox-regulated ATPase YchF -
  MMJJ_RS08560 (MMJJ_17240) - 1576149..1576712 (+) 564 WP_104838454.1 metal-dependent phosphohydrolase -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 18402.23 Da        Isoelectric Point: 9.1974

>NTDB_id=69 MMJJ_RS08540 WP_104838451.1 1571750..1572223(-) (MMJJ_17200) [Methanococcus maripaludis strain DSM 2067]
MNKKFNEYFLIILKYFSYSYIIYHILCHFESHLTNFIAYQSYLLLKLLLNDVILIQNLVYLPNIIISITEPCTGMMLISI
LLAHILTVENRLKYYVFGSLFCILLIYLGNIFRIVIIGILANTFGNGEYIHNTIGFVFFPTIAVFTILLWSKIKKRL

Nucleotide


Download         Length: 474 bp        

>NTDB_id=69 MMJJ_RS08540 WP_104838451.1 1571750..1572223(-) (MMJJ_17200) [Methanococcus maripaludis strain DSM 2067]
ATGAATAAAAAATTTAACGAATATTTTTTAATAATCTTAAAATATTTTAGTTATTCATATATAATTTACCATATATTATG
CCATTTTGAAAGTCATTTAACAAATTTTATAGCTTATCAAAGCTATTTATTGTTAAAACTTTTATTAAACGATGTAATTC
TGATTCAAAATCTTGTATATCTTCCAAATATCATAATTTCAATAACTGAACCATGTACTGGAATGATGTTGATTTCGATA
TTATTGGCTCATATATTGACGGTTGAAAACAGGTTAAAGTATTATGTTTTTGGTTCATTATTTTGTATATTATTAATATA
CCTTGGAAACATATTTAGAATCGTTATTATAGGAATCCTCGCAAATACGTTTGGAAATGGTGAATACATTCACAACACCA
TCGGTTTTGTGTTTTTTCCAACAATTGCAGTTTTTACGATTTTGCTATGGTCGAAAATCAAAAAAAGATTATAA

Domains

Predicted by InterproScan.

Transmembrane exosortase (Exosortase_EpsH) (Exosortase_EpsH)

(19-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L1CCK8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Dallas R Fonseca et al. (2023) Random transposon mutagenesis identifies genes essential for transformation in Methanococcus maripaludis. Molecular Genetics And Genomics : MGG 298(3):537-548. [PMID: 36823423]