Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   OSZ59_RS00730 Genome accession   NZ_CP113106
Coordinates   158471..158896 (-) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain BIAI 157     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 153471..163896
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OSZ59_RS00710 (OSZ59_00710) ileS 153628..156459 (+) 2832 WP_003102617.1 isoleucine--tRNA ligase -
  OSZ59_RS00715 (OSZ59_00715) lspA 156452..156961 (+) 510 WP_003102615.1 signal peptidase II -
  OSZ59_RS00720 (OSZ59_00720) fkpB 156954..157394 (+) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  OSZ59_RS00725 (OSZ59_00725) ispH 157480..158424 (+) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  OSZ59_RS00730 (OSZ59_00730) comF 158471..158896 (-) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  OSZ59_RS00735 (OSZ59_00735) pilY2 158893..159240 (-) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  OSZ59_RS00740 (OSZ59_00740) pilY1 159242..162733 (-) 3492 WP_003102607.1 type 4a pilus biogenesis protein PilY1 -
  OSZ59_RS00745 (OSZ59_00745) pilX 162745..163332 (-) 588 WP_003112826.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=689523 OSZ59_RS00730 WP_003094721.1 158471..158896(-) (comF) [Pseudomonas aeruginosa strain BIAI 157]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=689523 OSZ59_RS00730 WP_003094721.1 158471..158896(-) (comF) [Pseudomonas aeruginosa strain BIAI 157]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGGCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAATCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383