Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   M5E03_RS12555 Genome accession   NZ_CP097374
Coordinates   2429714..2432041 (-) Length   775 a.a.
NCBI ID   WP_252049636.1    Uniprot ID   -
Organism   Bacillus safensis strain AHB11     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2424714..2437041
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5E03_RS12535 (M5E03_12535) - 2424714..2426264 (+) 1551 WP_420831025.1 SH3 domain-containing protein -
  M5E03_RS12540 (M5E03_12540) dtd 2426363..2426806 (-) 444 WP_024423131.1 D-aminoacyl-tRNA deacylase -
  M5E03_RS12545 (M5E03_12545) - 2426825..2429035 (-) 2211 WP_047203353.1 RelA/SpoT family protein -
  M5E03_RS12550 (M5E03_12550) - 2429178..2429690 (-) 513 WP_095409180.1 adenine phosphoribosyltransferase -
  M5E03_RS12555 (M5E03_12555) recJ 2429714..2432041 (-) 2328 WP_252049636.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  M5E03_RS12560 (M5E03_12560) - 2432123..2432473 (-) 351 WP_039180309.1 LapA family protein -
  M5E03_RS12565 (M5E03_12565) - 2432575..2433063 (-) 489 WP_047203351.1 cation:proton antiporter regulatory subunit -
  M5E03_RS12570 (M5E03_12570) secDF 2433225..2435453 (-) 2229 WP_252049637.1 protein translocase subunit SecDF -
  M5E03_RS12575 (M5E03_12575) - 2435495..2435791 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 775 a.a.        Molecular weight: 87140.75 Da        Isoelectric Point: 4.9195

>NTDB_id=689271 M5E03_RS12555 WP_252049636.1 2429714..2432041(-) (recJ) [Bacillus safensis strain AHB11]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVKRGLEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKERSAIVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGELPVQLLDLAAIGTIADLVPLHDENRWL
AKKGLMQLRQSNRPGLKALLKEAGATLEEANEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDKDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEEAAGDDQSAIVVAKAGWNPGVVGIVASKLTDTFSRPAIVLGIDEETQMAKGSARSIPGFDLFF
HLSKCRDILPHFGGHPMAAGMTLRADDVALLRERLNQYANETLTEEDFIPIQRVDAVCKVDELTVQAIEEAGMLSPFGMS
NPKPFIMIEDAKLEDIRTIGANHNHIKMSLKDEDKLLDCVGFHQGKLKEEIVPGSRISVIGEMSVNEWNNRKKPQLMLKD
ARVDEWQLFDLRGKKDWEKKVSTLDSEKSLVVCFEEATKQNVDIPIHHVHAENVSTFDVHGKYVVFADVPADEQLLKQLL
ENQKPARIYTVFQREEDHFMSSFPSRDQFKWFYGFLFKRGSFPIKEQGMELAKHRGWTKDTIIFMTKVFFELGFVKIENG
VLSIVLDAPKKDLTASATYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2328 bp        

>NTDB_id=689271 M5E03_RS12555 WP_252049636.1 2429714..2432041(-) (recJ) [Bacillus safensis strain AHB11]
ATGTTATTATCCAAAATGCGCTGGGAATATGAACATCCAAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCGCTTCTCGTAAAAAGAGGACTTGAAGAAGTAGAAGAGGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTCTTATTAAAAGGAATGAAAGAAGCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGACTATGATGCAGATGGTGTGACAAGTACGTCTGTACTTTTACATACATTAAAAGAGCG
GTCTGCGATAGTAGATTTTTATATTCCAGATCGGTTTAAAGAAGGCTATGGGCCTAATGAACAGGCTTTTCGATATATCA
AGGAGCAAGGAGCATCGCTTGTCATCACGGTTGATACAGGCATTGCTGCTGTAAATGAAGCACGTATTGCAAAAGAAATA
GGGCTAGACCTGATCATTACAGATCACCATGAACCAAGTGAGGAGCTGCCTGAGGCACTTGCCATTGTCCATCCTAAGCA
GCCAGGCTGTGAGTATCCCTTTAAAGAACTTGCTGGTGTAGGTGTAGCGTTTAAAGTGGCGCATGCACTTCTTGGGGAGT
TGCCAGTTCAGCTGTTAGATCTTGCAGCAATCGGGACAATCGCGGATCTTGTACCGCTTCATGATGAGAATAGATGGCTC
GCCAAAAAAGGGCTTATGCAGCTTCGTCAGTCGAATAGACCGGGGCTGAAAGCGTTATTGAAAGAAGCGGGAGCCACTTT
AGAAGAAGCGAACGAGGAAACGGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTAGGACGAATTGAACAGGCAGATC
CAGCTGTTCACCTTCTTATGACTGAGGATAAGGATGAAGCAGAAGAGCTCGCTCGTTTTGTACAGGAACTCAATAAAGAA
CGACAAAAGATTGTCAGTACGATCACAGAAGAAGCCATTCAAATGGTGGAAGAGGCGGCAGGCGATGACCAGTCTGCGAT
CGTTGTAGCGAAAGCTGGCTGGAATCCGGGTGTTGTCGGAATTGTTGCCTCTAAATTGACGGATACTTTTTCTCGTCCGG
CGATTGTGCTTGGAATAGATGAAGAAACGCAAATGGCAAAAGGATCAGCCCGGAGCATTCCTGGCTTTGATCTATTCTTC
CATCTAAGCAAATGCCGCGACATCCTGCCGCATTTCGGCGGGCATCCGATGGCAGCAGGGATGACACTTCGAGCGGATGA
TGTCGCTTTACTAAGGGAAAGATTAAATCAATATGCCAATGAAACATTGACGGAAGAAGATTTTATTCCCATTCAGCGGG
TCGATGCTGTGTGTAAGGTAGACGAATTGACGGTTCAAGCGATTGAAGAAGCAGGGATGCTGTCACCATTTGGTATGTCA
AACCCAAAACCGTTCATTATGATTGAAGATGCGAAGCTTGAAGATATCCGAACAATTGGGGCAAATCACAACCATATTAA
AATGTCTCTGAAAGACGAAGACAAGCTGCTTGACTGTGTCGGTTTTCACCAAGGGAAATTAAAAGAAGAAATTGTTCCAG
GAAGCCGCATTTCCGTGATTGGAGAAATGTCTGTCAATGAATGGAATAACCGCAAAAAGCCCCAGCTAATGCTAAAAGAT
GCACGAGTTGATGAATGGCAGCTGTTTGATCTGCGAGGGAAAAAGGATTGGGAGAAGAAAGTTTCGACGCTTGATTCTGA
AAAATCTTTGGTTGTCTGTTTTGAGGAAGCAACAAAACAGAACGTCGATATCCCCATTCATCATGTTCATGCAGAAAATG
TATCAACGTTTGATGTACACGGAAAGTATGTGGTCTTTGCGGATGTTCCCGCAGATGAGCAATTGCTGAAGCAGCTTTTA
GAAAATCAAAAGCCTGCCAGAATTTATACGGTTTTTCAAAGAGAAGAAGATCATTTTATGTCCTCTTTCCCAAGCCGTGA
TCAATTCAAATGGTTTTATGGGTTCTTGTTTAAACGGGGAAGTTTTCCTATCAAAGAACAAGGAATGGAGCTTGCCAAAC
ATAGAGGCTGGACAAAAGATACAATCATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAGAATGGT
GTGCTGTCAATAGTACTTGATGCCCCTAAAAAAGATTTAACGGCATCAGCGACCTATACAGCAAAACAACGATTGATGGA
ACTCGATCAAACACTAACATATTCCTCAGCTAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCTCACCTG
TGCTTTGA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(314-438)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(567-764)

RecJ OB domain (RecJ_OB)

(454-560)

DHH family (DHH)

(83-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.738

100

0.68