Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   M5E02_RS12385 Genome accession   NZ_CP097373
Coordinates   2417880..2420204 (-) Length   774 a.a.
NCBI ID   WP_252056380.1    Uniprot ID   -
Organism   Bacillus safensis strain AHB2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2412880..2425204
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5E02_RS12370 (M5E02_12370) dtd 2414529..2414972 (-) 444 WP_048238991.1 D-aminoacyl-tRNA deacylase -
  M5E02_RS12375 (M5E02_12375) - 2414991..2417201 (-) 2211 WP_047203353.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  M5E02_RS12380 (M5E02_12380) - 2417344..2417856 (-) 513 WP_024423133.1 adenine phosphoribosyltransferase -
  M5E02_RS12385 (M5E02_12385) recJ 2417880..2420204 (-) 2325 WP_252056380.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  M5E02_RS12390 (M5E02_12390) - 2420286..2420636 (-) 351 WP_252056381.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  M5E02_RS12395 (M5E02_12395) - 2420738..2421226 (-) 489 WP_041115109.1 cation:proton antiporter regulatory subunit -
  M5E02_RS12400 (M5E02_12400) secDF 2421413..2423641 (-) 2229 WP_252056382.1 protein translocase subunit SecDF -
  M5E02_RS12405 (M5E02_12405) - 2423683..2423979 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 87034.65 Da        Isoelectric Point: 4.9843

>NTDB_id=689211 M5E02_RS12385 WP_252056380.1 2417880..2420204(-) (recJ) [Bacillus safensis strain AHB2]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVKRGLEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKERSAIVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPGEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGKLPVQLLDLAAIGTIADLVPLHDENRWL
AKKGLVQLRQSNRPGLKALLKEAGATLEEASEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDKDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEETHDDQSAIVVAKAGWNPGVVGIVASKLTDTFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLQADDVALLRERLNQYANETLTEEDFIPIQRVDAVCKVDELTVEAIEEAGMLSPFGMSN
PKPFIMIEDAKLEDIRTIGANHNHIKMSLKDDEKLLDCVGFHQGKLKEEIVPGSRISVIGEMSVNEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKKVSTLDSEKSLVVCFDEATKQDVDIPIHHVHAENVSTFDVHGKYVVFADVPADEQLLKQLLE
NQKPARIYTVFQRKEDHFMSSFPSRDQFKWFYGFLFKRGSFPIKEQGMELAKHRGWTKDTIIFMTKVFFELGFVKIENGV
LSIVLDAPKKDLTASASYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=689211 M5E02_RS12385 WP_252056380.1 2417880..2420204(-) (recJ) [Bacillus safensis strain AHB2]
ATGTTATTATCCAAAATGCGCTGGGAATATGAACATCCAAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCGCTTCTCGTAAAAAGAGGACTTGAAGAAGTAGAAGAGGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTCCTATTAAAAGGAATGAAAGAAGCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGACTATGATGCAGATGGTGTGACAAGTACGTCTGTACTTTTACATACATTAAAAGAGCG
GTCTGCGATAGTAGACTTTTATATTCCAGATCGATTTAAAGAAGGCTATGGGCCTAACGAACAGGCATTTCGATATATCA
AGGAGCAAGGAGCATCGCTTGTCATCACGGTCGATACAGGCATTGCTGCTGTCAATGAAGCACGTATTGCAAAAGAAATA
GGATTAGACCTAATCATTACAGATCACCATGAACCAGGTGAGGAGCTGCCTGAGGCACTTGCTATTGTCCATCCTAAGCA
GCCGGGCTGTGAGTATCCCTTTAAAGAACTTGCTGGTGTAGGTGTCGCGTTTAAAGTGGCGCATGCACTTCTTGGGAAGC
TGCCAGTCCAGCTGTTAGATCTTGCAGCAATCGGGACAATCGCGGATCTTGTACCGCTTCATGATGAGAATAGGTGGCTC
GCCAAAAAAGGCCTTGTGCAGCTTCGGCAGTCGAATAGACCAGGGCTGAAAGCATTATTGAAAGAAGCGGGAGCCACTCT
AGAAGAAGCGAGCGAGGAAACGGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTAGGGCGAATTGAACAGGCAGATC
CTGCCGTTCATCTTCTTATGACTGAGGATAAGGATGAAGCAGAAGAGCTTGCTCGTTTTGTACAGGAACTGAATAAAGAA
CGACAAAAGATTGTCAGTACGATCACAGAAGAAGCCATTCAAATGGTGGAAGAGACGCATGATGACCAGTCTGCGATCGT
TGTAGCGAAAGCTGGCTGGAATCCGGGCGTTGTCGGAATTGTTGCCTCTAAATTGACGGATACTTTTTCTCGTCCGGCGA
TTGTGCTTGGAATAGATGAAGAGACGCAAATGGCAAAAGGATCAGCCCGGAGTATTCCTGGCTTCGATTTATTTTTCCAT
CTAAGCAAATGCCGCGACATCCTGCCGCATTTCGGCGGGCACCCGATGGCAGCTGGAATGACACTTCAAGCGGATGATGT
CGCTTTACTAAGGGAAAGATTAAATCAATATGCAAATGAAACATTGACGGAAGAAGATTTTATTCCAATTCAGCGGGTTG
ATGCAGTGTGTAAGGTAGACGAATTGACGGTTGAAGCGATTGAAGAAGCGGGGATGCTGTCACCATTCGGTATGTCAAAC
CCAAAACCGTTCATTATGATTGAAGATGCGAAGCTTGAAGATATCCGAACGATTGGTGCAAATCACAACCATATTAAAAT
GTCTCTGAAAGACGATGAAAAGCTGCTTGACTGTGTCGGTTTTCACCAAGGGAAATTAAAAGAAGAAATTGTTCCAGGAA
GCCGCATCTCCGTGATTGGGGAAATGTCCGTCAATGAATGGAATAACCGGAAGAAACCCCAGCTGATGTTAAAAGATGCA
CGAGTTGATGAATGGCAGCTGTTTGATCTGCGCGGGAAAAAGGATTGGGAGAAGAAAGTTTCGACGCTTGATTCAGAAAA
ATCTTTGGTTGTCTGTTTTGATGAAGCAACAAAACAGGACGTCGATATCCCCATTCATCATGTTCATGCAGAAAATGTAT
CAACGTTTGATGTACACGGAAAGTATGTGGTCTTTGCGGATGTTCCCGCAGATGAGCAACTGCTGAAGCAGCTTTTAGAA
AACCAAAAGCCTGCCAGAATATATACGGTTTTTCAAAGAAAAGAAGACCATTTTATGTCCTCTTTCCCAAGCCGTGACCA
ATTCAAATGGTTTTATGGGTTCTTGTTTAAACGAGGAAGTTTTCCTATCAAAGAACAAGGAATGGAGCTTGCCAAACATA
GAGGCTGGACAAAAGATACAATCATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAGAATGGTGTG
CTGTCAATAGTACTTGATGCCCCTAAAAAAGATTTAACGGCATCAGCGTCCTATACAGCAAAACAACGATTGATGGAACT
CGATCAAACACTAACATATTCCTCAGCTAAAGAGCTGAAGGAGTGGCTGAATAGCATGATGAGCGAAGTCTCACCTGTGC
TTTGA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-763)

DHHA1 domain (DHHA1)

(309-437)

DHH family (DHH)

(83-226)

RecJ OB domain (RecJ_OB)

(453-559)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.31

100

0.676