Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   M5J22_RS12935 Genome accession   NZ_CP097326
Coordinates   2672574..2674934 (-) Length   786 a.a.
NCBI ID   WP_201489024.1    Uniprot ID   -
Organism   Bacillus velezensis strain GUCC45     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2667574..2679934
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5J22_RS12915 (M5J22_12915) - 2667649..2669211 (+) 1563 WP_015387900.1 SH3 domain-containing protein -
  M5J22_RS12920 (M5J22_12920) dtd 2669238..2669681 (-) 444 WP_007408194.1 D-aminoacyl-tRNA deacylase -
  M5J22_RS12925 (M5J22_12925) relA 2669694..2671898 (-) 2205 WP_003152716.1 GTP diphosphokinase -
  M5J22_RS12930 (M5J22_12930) - 2672056..2672568 (-) 513 WP_003152714.1 adenine phosphoribosyltransferase -
  M5J22_RS12935 (M5J22_12935) recJ 2672574..2674934 (-) 2361 WP_201489024.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  M5J22_RS12940 (M5J22_12940) - 2674990..2675316 (-) 327 WP_003152709.1 lipopolysaccharide assembly LapA domain-containing protein -
  M5J22_RS12945 (M5J22_12945) - 2675380..2675877 (-) 498 WP_003152707.1 cation:proton antiporter regulatory subunit -
  M5J22_RS12950 (M5J22_12950) secDF 2676009..2678228 (-) 2220 WP_003152704.1 protein translocase subunit SecDF -
  M5J22_RS12955 (M5J22_12955) - 2678265..2678561 (-) 297 WP_003152702.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 786 a.a.        Molecular weight: 87451.80 Da        Isoelectric Point: 4.8923

>NTDB_id=688482 M5J22_RS12935 WP_201489024.1 2672574..2674934(-) (recJ) [Bacillus velezensis strain GUCC45]
MLASKMRWEIQQPDQEKVKSLTEQLDITPLVASLLVKRGFDTAESARLFLHTKDADFYDPYELKGMREAVDRIKLAVSQG
EPIMIYGDYDADGVTSTSVMLTALRQLSADVDFYIPDRFTEGYGPNEQAFRSIKERGFSLIITVDTGIAAVHEAEVAKEL
GLDVIITDHHEPGPELPDVFAIIHPKQPGCGYPFKELAGVGVAFKLAHALTGSLPEAMLDLAAIGTVADLVPLHDENRLI
ASLGIERLRTTNRPGLKALIKLSGGDIGQADEETIGFQVAPRLNAVGRIEQADPAVHLLMTEDQFEAEELASDIDRLNKE
RQKMVKTMTEEAVEMVQQQGGNPSAIVVEKAGWNPGVVGIVASKLAERFYRPAVVLGIDEEQGIAKGSARSIRGFNLFTS
LSQCRDILPHFGGHPMAAGMTLKAEDVAELRDRLNQMAEETLTEEDFIPVQEVDLVCGVEDITVESITELNMLSPFGMLN
PKPHVMVKNAVLDDVRLIGANKNHVKMTVKSDSAVLDCVGFNKGELEAGVVPGSTISIVGEMSINEWNNRKKPQLMIKDA
AVPEWQLFDIRGKRTWEDTVAALPKEKRVLISFNEKTAQKLREKELTGELAEITSESEAEALQLDGKYAVFLDVPPSADR
LQHLLDGKKPERIYFVFINHEEHFLSSFPSRNDFKWFYAFLLKRGAFDFGKHGSELARHRGWSIDTINFMTKVFFDLGFV
KIEDGVLSVVNGAKKRDLADSQTYQVKQQLMELDQKLNYSAAHELKEWLNKLMNQGSEAYESTRRT

Nucleotide


Download         Length: 2361 bp        

>NTDB_id=688482 M5J22_RS12935 WP_201489024.1 2672574..2674934(-) (recJ) [Bacillus velezensis strain GUCC45]
ATGTTAGCGTCAAAAATGCGCTGGGAAATCCAGCAGCCGGATCAGGAAAAAGTCAAATCACTCACAGAACAATTGGACAT
AACACCTCTTGTGGCATCACTGCTTGTAAAAAGAGGTTTTGATACTGCCGAAAGCGCAAGGCTGTTTTTACATACAAAGG
ATGCAGACTTTTATGACCCGTATGAATTAAAGGGAATGCGGGAAGCAGTTGATCGGATAAAACTGGCGGTTTCTCAAGGG
GAACCGATTATGATTTACGGAGATTACGATGCAGACGGCGTTACAAGCACGTCAGTGATGCTGACGGCACTCAGACAGCT
GTCGGCGGACGTTGACTTTTATATTCCCGACCGTTTTACAGAAGGCTACGGCCCGAATGAACAAGCTTTCCGTTCCATAA
AAGAGCGGGGATTTTCGCTGATCATTACAGTGGATACGGGAATCGCCGCCGTCCATGAAGCTGAAGTCGCAAAAGAGCTG
GGCTTAGACGTCATTATTACCGACCACCATGAGCCGGGACCCGAGCTTCCTGACGTATTTGCGATCATCCACCCGAAGCA
GCCGGGATGCGGCTATCCGTTTAAAGAATTGGCCGGCGTCGGTGTGGCGTTTAAGCTTGCCCACGCGCTTACGGGTTCTT
TGCCGGAAGCTATGCTGGACTTGGCGGCAATCGGTACGGTTGCAGATCTGGTGCCCCTTCATGATGAAAACAGATTAATT
GCGTCACTCGGAATTGAAAGGCTTCGGACGACAAATCGGCCGGGCCTCAAAGCTCTGATTAAGCTTTCCGGCGGAGATAT
CGGACAAGCTGATGAGGAAACGATCGGATTTCAGGTCGCACCGAGGCTCAATGCTGTCGGAAGAATAGAACAGGCCGATC
CTGCCGTACACCTGCTGATGACCGAAGATCAGTTTGAAGCGGAGGAACTGGCTTCGGATATTGACCGGCTGAATAAAGAG
CGGCAGAAAATGGTGAAAACCATGACCGAAGAGGCTGTTGAAATGGTTCAGCAGCAAGGCGGAAACCCGTCTGCCATTGT
AGTGGAAAAAGCCGGCTGGAATCCGGGTGTCGTCGGTATTGTCGCATCGAAGCTGGCTGAACGTTTTTACCGGCCCGCTG
TTGTGCTTGGGATAGATGAGGAGCAGGGAATCGCCAAAGGGTCGGCGCGAAGCATCAGAGGCTTCAACCTTTTTACGAGC
CTTTCACAGTGCAGAGACATTCTTCCTCATTTCGGCGGGCATCCGATGGCGGCCGGAATGACGCTGAAGGCTGAAGATGT
GGCGGAATTAAGGGACAGGCTGAATCAAATGGCTGAAGAAACCCTTACGGAAGAGGATTTTATTCCCGTTCAGGAGGTTG
ATCTTGTCTGCGGCGTTGAAGACATAACCGTTGAAAGCATTACAGAATTGAATATGCTGTCGCCGTTCGGCATGCTTAAC
CCTAAACCGCATGTGATGGTGAAAAATGCCGTGCTTGATGATGTGCGCCTGATCGGAGCGAATAAAAACCACGTGAAAAT
GACGGTGAAAAGTGATTCCGCCGTCCTTGACTGCGTCGGCTTTAACAAAGGAGAGCTGGAAGCCGGTGTTGTTCCCGGAT
CAACGATTTCCATTGTCGGCGAGATGTCCATTAATGAATGGAACAACAGAAAGAAGCCCCAGCTGATGATTAAAGATGCT
GCGGTGCCGGAATGGCAGCTTTTTGATATCCGGGGCAAGCGGACGTGGGAAGATACGGTCGCCGCGCTGCCGAAGGAGAA
ACGGGTGCTCATCAGCTTTAACGAAAAAACCGCTCAGAAGCTCCGTGAAAAAGAGCTGACGGGCGAGCTTGCCGAGATCA
CTTCGGAATCCGAGGCGGAAGCTCTCCAGCTTGACGGAAAATATGCCGTTTTTCTGGATGTGCCGCCGTCAGCGGATCGC
CTGCAGCATTTGCTTGACGGAAAGAAGCCGGAACGGATTTATTTTGTTTTTATCAATCATGAAGAACATTTCCTGTCCTC
ATTTCCATCGCGGAATGATTTCAAGTGGTTTTACGCATTTCTCTTGAAAAGGGGCGCTTTTGACTTCGGCAAGCATGGTT
CAGAGCTTGCGAGACACAGAGGATGGTCAATTGACACAATCAATTTCATGACAAAGGTGTTTTTTGATCTCGGTTTTGTT
AAAATAGAAGATGGTGTGCTGTCTGTCGTGAACGGTGCGAAAAAACGCGACCTGGCAGATTCGCAAACCTATCAGGTAAA
ACAGCAGCTGATGGAATTAGACCAGAAGCTCAATTATTCCGCAGCCCATGAGCTGAAGGAATGGCTGAATAAGCTGATGA
ACCAAGGTTCAGAGGCTTATGAAAGTACGAGGAGGACATAG

Domains

Predicted by InterproScan.

DHH family (DHH)

(83-226)

DHHA1 domain (DHHA1)

(315-437)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-769)

RecJ OB domain (RecJ_OB)

(453-559)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

81.807

100

0.818