Detailed information NTG0068832

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: CFK21_RS02955
• Genome accession: NZ_AP018052
• Coordinates: 633762..634940 (-)
• Length: 392 a.a.
• NCBI ID: WP_096364588.1
• Uniprot ID: A0A1Z4VN36
• Organism: Thiohalobacter thiocyanaticus strain FOKN1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 628762..639940

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CFK21_RS02935 (FOKN1_0601)	groES	629580..629870 (+)	291	WP_096364580.1	co-chaperone GroES	-
CFK21_RS02940 (FOKN1_0602)	groL	629916..631568 (+)	1653	WP_096364582.1	chaperonin GroEL	-
CFK21_RS02945 (FOKN1_0603)	-	631934..632704 (-)	771	WP_096364584.1	hypothetical protein	-
CFK21_RS02950 (FOKN1_0604)	-	632704..633267 (-)	564	WP_096364586.1	RNA polymerase sigma factor	-
CFK21_RS02955 (FOKN1_0605)	pilU	633762..634940 (-)	1179	WP_096364588.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
CFK21_RS02960 (FOKN1_0606)	glnE	635225..638131 (+)	2907	WP_231971550.1	bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase	-
CFK21_RS02965 (FOKN1_0607)	-	638354..639274 (+)	921	WP_096364590.1	branched-chain amino acid transaminase	-
CFK21_RS02970 (FOKN1_0608)	-	639659..639883 (+)	225	WP_096364592.1	zinc-finger domain-containing protein	-

Sequence

Protein

• Length: 392 a.a.
• Molecular weight: 43850.01 Da
• Isoelectric Point: 5.1153

>NTDB_id=68832 CFK21_RS02955 WP_096364588.1 633762..634940(-) (pilU) [Thiohalobacter thiocyanaticus strain FOKN1]
MEFKDYLKIMVMKDASDLYLSTGAPPCAKFHGQLKVVEKATMTPERIREIAYSLMSETQQKDFEKKPEMNLAISEHNVGR
FRVNIFKQRNSIGMVVRNIKTDIPAWQDLGLPPILTDVLMSKRGLVLFVGATGSGKSTSLASLIDYRNTNATGHIITIED
PIEFIHSHKKSIVNQREVGLDTDSYEDALANTLRQAPDVILIGEIRHRETMEHAISFAETGHLCLSTLHANNANQALDRI
INFFPEEKRDQLLLDLSLNLRAFISQRLVQTVDGQRTAAIEILLGTPLVKELVRRGDVHEIKEVMEKSENLGMQTFDMAL
YNLYMEGRISLEEALRNADSPNNLRLRINLTAEDAEQALAEKSESFSLEEDDTPSSNIATAGGGLGLKLADE

Nucleotide

• Length: 1179 bp

>NTDB_id=68832 CFK21_RS02955 WP_096364588.1 633762..634940(-) (pilU) [Thiohalobacter thiocyanaticus strain FOKN1]
ATGGAATTCAAGGATTATCTCAAGATCATGGTCATGAAGGACGCCTCCGACCTCTACCTGAGCACGGGGGCGCCGCCCTG
CGCCAAGTTCCATGGCCAACTCAAGGTGGTGGAGAAGGCCACCATGACGCCGGAGCGCATCCGCGAGATCGCCTACTCGC
TGATGAGCGAAACCCAGCAGAAGGACTTCGAGAAGAAGCCGGAAATGAACCTGGCCATCTCCGAGCACAATGTCGGCCGC
TTCCGCGTCAACATCTTCAAGCAGCGCAACAGCATCGGCATGGTGGTGCGCAACATCAAGACCGATATCCCGGCGTGGCA
GGACCTGGGGCTGCCGCCCATCCTCACCGACGTGTTGATGAGCAAGCGCGGCCTGGTGCTGTTCGTGGGCGCGACCGGCT
CGGGCAAGTCCACCTCGCTGGCCTCGCTGATCGACTATCGCAACACCAACGCCACCGGCCACATCATCACCATCGAGGAT
CCGATCGAGTTCATCCACTCGCACAAGAAGTCCATCGTCAACCAGCGCGAGGTCGGGCTGGACACCGATTCCTATGAAGA
CGCCCTGGCCAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCCATCGCGAGACCATGGAGCATG
CCATCTCCTTCGCCGAGACCGGCCACCTCTGCCTGTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAGAAGCGCGACCAGTTGCTGCTGGACCTGTCGCTGAACCTGCGCGCCTTCATCTCCCAGCG
CCTGGTGCAGACCGTCGACGGCCAGCGCACCGCGGCGATCGAGATCCTGCTGGGCACCCCGCTGGTCAAGGAACTGGTGC
GCCGCGGCGATGTGCACGAGATCAAGGAGGTCATGGAGAAATCCGAGAACCTCGGCATGCAGACCTTCGACATGGCGCTG
TACAACCTCTACATGGAAGGCCGGATCAGCCTGGAAGAGGCGCTGCGCAATGCCGACTCCCCCAACAACCTGCGCCTGCG
CATCAATCTCACGGCCGAGGACGCCGAGCAGGCCCTGGCCGAGAAGAGCGAATCCTTCAGTCTGGAGGAGGACGATACCC
CGAGCAGCAACATCGCCACCGCCGGCGGCGGGCTGGGGTTGAAGCTGGCGGACGAATGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (14-270)

3D structure

• AlphaFold DB: A0A1Z4VN36

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.355	96.173	0.561
pilU	Acinetobacter baylyi ADP1	54.648	90.561	0.495
pilU	Vibrio cholerae strain A1552	52.45	88.52	0.464
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.239	100	0.423