Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   EFK13_RS14020 Genome accession   NZ_CP096889
Coordinates   2731967..2734327 (-) Length   786 a.a.
NCBI ID   WP_129508050.1    Uniprot ID   -
Organism   Bacillus cabrialesii strain TE3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2726967..2739327
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EFK13_RS14000 (EFK13_14000) - 2727144..2728496 (+) 1353 WP_129508246.1 IS1182 family transposase -
  EFK13_RS14005 (EFK13_14005) dtd 2728624..2729064 (-) 441 WP_129508051.1 D-aminoacyl-tRNA deacylase -
  EFK13_RS14010 (EFK13_14010) relA 2729077..2731281 (-) 2205 WP_103748189.1 GTP diphosphokinase -
  EFK13_RS14015 (EFK13_14015) - 2731449..2731961 (-) 513 WP_010331008.1 adenine phosphoribosyltransferase -
  EFK13_RS14020 (EFK13_14020) recJ 2731967..2734327 (-) 2361 WP_129508050.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  EFK13_RS14025 (EFK13_14025) - 2734394..2734717 (-) 324 WP_129508049.1 lipopolysaccharide assembly LapA domain-containing protein -
  EFK13_RS14030 (EFK13_14030) - 2734790..2735293 (-) 504 WP_129508048.1 cation:proton antiporter regulatory subunit -
  EFK13_RS14035 (EFK13_14035) secDF 2735449..2737662 (-) 2214 WP_129508047.1 protein translocase subunit SecDF -
  EFK13_RS14040 (EFK13_14040) comN 2737701..2737997 (-) 297 WP_019259048.1 post-transcriptional regulator ComN -

Sequence

Protein


Download         Length: 786 a.a.        Molecular weight: 88147.75 Da        Isoelectric Point: 5.0075

>NTDB_id=684892 EFK13_RS14020 WP_129508050.1 2731967..2734327(-) (recJ) [Bacillus cabrialesii strain TE3]
MLASKMRWEIQRPDQDKVKSLTEQLHITPLVASLLVKRGFDTAESAKLFLHTKDADFYDPFEMKGMREAAERIKQAISQQ
EQIMIYGDYDADGVTSTSVMLHTLQKLSAQVDFYIPDRFKEGYGPNEQAFRSIKERGFSLIITVDTGIAAVHEANVAKEL
GLDVIITDHHEPGPELPDVHAIVHPKQPGCTYPFKELAGVGVAFKLAHALLGELPDELLDLAAIGTIADLVPLHDENRLI
ATLGLERLRRTNRLGLKELIKLSGGDIGEANEETVGFQLAPRLNAVGRIEQADPAVHLLMSEDLFEAEELAAEIDQLNKE
RQKMVSKMTDEAIDMVEQQGLDQTAIVVAKAGWNPGVVGIVASKLVDRFYRPAIVLGIDEEKGIAKGSARSIRGFNLFES
LSECRDILPHFGGHPMAAGMTLNAEDVPDLRNRLNEIADNTLTEEDFIPVQEIDLVCGVEDITVESITEMNLLSPFGMLN
PKPHVLVENAVLEDVRKIGANKNHVKMTVRNESSQLDCVGFHKGELEESIVPGSRISIVGEMSINEWNNRKKPQLMIKDA
AVSEWQLFDLRGKRMWENTVSALPAAKRAIVSFKEDSTTVLQAEELRREVHVISSEEQAKALDLNGAYIVLLDPPPSLDM
LARLLEGKAPERIYFIFLNQEDHFLSTFPARDHFKWYYAFLLKRGTFDVKKHGSELARHKGWSVETINFMTKVFFDLGFV
KIENGVLSVVSGVKKRDLTDSQTYQAKQQLMELDQKLNYSSAEELKEWLNKLMKQDSEAYESTRRT

Nucleotide


Download         Length: 2361 bp        

>NTDB_id=684892 EFK13_RS14020 WP_129508050.1 2731967..2734327(-) (recJ) [Bacillus cabrialesii strain TE3]
ATGTTAGCGTCAAAAATGCGATGGGAAATCCAGCGACCAGATCAGGATAAGGTCAAATCACTCACAGAACAATTGCATAT
AACACCTCTTGTTGCGTCCCTGCTTGTAAAAAGAGGCTTTGATACGGCAGAAAGCGCAAAGCTGTTTTTACATACAAAGG
ATGCCGATTTTTATGACCCTTTTGAAATGAAGGGCATGAGAGAAGCGGCAGAGCGAATAAAACAGGCGATTTCACAACAA
GAACAGATTATGATATATGGCGATTATGACGCCGATGGAGTTACCAGTACGTCAGTGATGCTGCACACGCTGCAAAAGCT
GTCGGCTCAAGTTGATTTTTATATACCTGACCGCTTTAAAGAAGGATATGGCCCTAATGAACAGGCGTTTCGTTCAATTA
AAGAACGAGGTTTCTCTCTGATTATTACGGTTGATACAGGAATTGCCGCTGTGCATGAAGCGAATGTGGCAAAGGAACTC
GGACTTGATGTCATCATTACCGATCACCATGAGCCGGGACCGGAGCTTCCGGATGTCCACGCGATCGTCCACCCGAAGCA
GCCGGGCTGCACCTACCCGTTTAAAGAACTGGCCGGGGTCGGCGTCGCGTTTAAGCTTGCCCATGCTTTACTGGGCGAAC
TCCCGGATGAACTGCTTGACCTGGCGGCAATCGGCACCATTGCCGATCTTGTCCCGTTGCATGACGAAAACAGATTAATC
GCAACACTGGGACTTGAAAGGCTTCGGCGGACGAATCGGCTGGGCTTAAAAGAGCTGATCAAGCTGTCAGGCGGAGATAT
CGGAGAAGCGAACGAAGAAACAGTCGGGTTCCAGTTGGCACCAAGGCTGAATGCCGTCGGAAGAATTGAACAGGCTGATC
CTGCTGTTCATTTGCTCATGTCAGAGGATTTGTTTGAAGCGGAAGAGCTTGCCGCTGAAATTGACCAGCTGAACAAAGAG
CGCCAAAAGATGGTCAGCAAAATGACGGATGAAGCGATTGATATGGTTGAACAGCAAGGTCTCGATCAAACTGCAATCGT
CGTGGCCAAAGCCGGATGGAATCCAGGAGTCGTCGGGATTGTGGCTTCAAAGCTTGTGGACCGTTTCTACCGCCCGGCCA
TTGTGCTTGGCATTGATGAAGAAAAAGGGATCGCTAAAGGCTCGGCCAGAAGCATCAGGGGCTTTAATTTATTCGAAAGC
CTTTCTGAATGCAGAGATATTCTTCCTCATTTCGGCGGCCATCCAATGGCGGCGGGAATGACGCTAAATGCCGAAGATGT
GCCGGATTTGCGAAACAGGCTGAATGAAATAGCGGACAACACGCTTACGGAAGAGGATTTTATTCCTGTACAGGAAATCG
ATCTCGTCTGCGGCGTCGAGGACATTACGGTTGAGAGCATCACAGAGATGAATCTGCTGTCTCCATTTGGCATGCTGAAC
CCGAAACCGCACGTTCTCGTAGAAAATGCTGTGCTGGAAGATGTCCGCAAGATCGGAGCTAATAAAAATCATGTCAAAAT
GACGGTCAGAAACGAGTCTTCTCAGCTTGATTGCGTGGGGTTTCATAAAGGCGAGCTTGAAGAAAGCATCGTCCCGGGTT
CAAGAATATCAATTGTCGGTGAGATGTCAATCAACGAATGGAACAATAGAAAAAAACCGCAGCTAATGATTAAGGATGCC
GCTGTTTCTGAATGGCAGCTGTTTGATTTAAGGGGAAAACGGATGTGGGAAAACACAGTTTCCGCTCTACCAGCTGCTAA
ACGGGCCATTGTCTCTTTCAAAGAAGACTCAACAACAGTTCTTCAGGCTGAAGAACTTCGCCGTGAAGTGCACGTCATCA
GCTCTGAAGAACAAGCGAAGGCGTTAGACCTTAATGGCGCATATATTGTATTGCTGGATCCGCCGCCTTCGTTGGACATG
CTCGCACGTTTGCTGGAAGGCAAGGCGCCTGAGCGGATATATTTCATTTTTCTCAATCAAGAGGATCACTTTTTATCAAC
ATTTCCCGCAAGAGACCACTTCAAATGGTATTATGCGTTCTTGCTGAAAAGAGGCACTTTTGACGTAAAAAAACACGGTT
CAGAGCTTGCAAGACATAAAGGCTGGTCCGTGGAAACAATCAATTTCATGACAAAGGTGTTTTTTGATCTAGGTTTTGTT
AAAATAGAGAATGGTGTGCTGTCTGTGGTTAGCGGAGTAAAGAAACGCGACTTAACAGACTCACAAACATATCAAGCGAA
ACAGCAGCTGATGGAACTGGATCAAAAGCTGAATTATTCTTCTGCTGAAGAGCTGAAGGAATGGCTGAATAAGCTGATGA
AACAAGATTCAGAGGCTTATGAAAGTACGAGGAGGACATAA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(315-437)

RecJ OB domain (RecJ_OB)

(453-559)

DHH family (DHH)

(82-226)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-769)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

95.929

100

0.959