Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   PALA35_RS25745 Genome accession   NZ_CP110346
Coordinates   5531933..5532358 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain PALA35     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5526933..5537358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA35_RS25730 (PALA35_05092) pilX 5527497..5528084 (+) 588 WP_003094700.1 type 4a pilus minor pilin PilX -
  PALA35_RS25735 (PALA35_05093) pilY1 5528096..5531587 (+) 3492 WP_269974498.1 type 4a pilus biogenesis protein PilY1 -
  PALA35_RS25740 (PALA35_05094) pilY2 5531589..5531936 (+) 348 WP_003094713.1 type 4a fimbrial biogenesis protein PilY2 -
  PALA35_RS25745 (PALA35_05095) comF 5531933..5532358 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  PALA35_RS25750 (PALA35_05096) ispH 5532403..5533347 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  PALA35_RS25755 (PALA35_05097) fkpB 5533433..5533873 (-) 441 WP_269974499.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  PALA35_RS25760 (PALA35_05098) lspA 5533866..5534375 (-) 510 WP_003094728.1 signal peptidase II -
  PALA35_RS25765 (PALA35_05099) ileS 5534368..5537199 (-) 2832 WP_003102617.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=683902 PALA35_RS25745 WP_003094721.1 5531933..5532358(+) (comF) [Pseudomonas aeruginosa strain PALA35]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=683902 PALA35_RS25745 WP_003094721.1 5531933..5532358(+) (comF) [Pseudomonas aeruginosa strain PALA35]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGCTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCACAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383