Detailed information NTG0683314

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: M1S16_RS14985
• Genome accession: NZ_CP096729
• Coordinates: 3086369..3087487 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain 5729
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3081369..3092487

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M1S16_RS14975 (M1S16_14975)	-	3084485..3085177 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
M1S16_RS14980 (M1S16_14980)	pilT	3085304..3086341 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
M1S16_RS14985 (M1S16_14985)	pilU	3086369..3087487 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
M1S16_RS14990 (M1S16_14990)	fur	3087557..3087994 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
M1S16_RS14995 (M1S16_14995)	bamE	3088106..3088504 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
M1S16_RS15000 (M1S16_15000)	-	3088559..3088882 (-)	324	WP_000442587.1	RnfH family protein	-
M1S16_RS15005 (M1S16_15005)	-	3088882..3089949 (-)	1068	WP_002019572.1	hypothetical protein	-
M1S16_RS15010 (M1S16_15010)	-	3090102..3090557 (-)	456	WP_000782976.1	bacteriohemerythrin	-
M1S16_RS15015 (M1S16_15015)	-	3090810..3091658 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=683314 M1S16_RS14985 WP_000347039.1 3086369..3087487(+) (pilU) [Acinetobacter baumannii strain 5729]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=683314 M1S16_RS14985 WP_000347039.1 3086369..3087487(+) (pilU) [Acinetobacter baumannii strain 5729]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395