Detailed information    

experimental Experimentally validated

Overview

Name   ecnA   Type   Unclear
Locus tag   MMJJ_RS08165 Genome accession   NZ_CP026606
Coordinates   1499355..1499912 (+) Length   185 a.a.
NCBI ID   WP_104838392.1    Uniprot ID   A0A2L1CCC5
Organism   Methanococcus maripaludis strain DSM 2067     
Function   require for natural transformation   
Unclear

Function

In-frame deletion mutants for MMJJ_16440 were completely defective for transformation, and complementation of the mutation in trans rescued this defect. EcnA is Ca2+-activated, present on the cell surface, and essential for transformation. While EcnA can degrade several forms of DNA, the highest activity was observed with ssDNA as a substrate. Activity was also observed with circular dsDNA, suggesting that EcnA is an endonuclease.


Genomic Context

Location: 1494355..1504912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMJJ_RS08145 (MMJJ_16400) aroA 1495446..1496735 (+) 1290 WP_104838388.1 3-phosphoshikimate 1-carboxyvinyltransferase -
  MMJJ_RS08150 (MMJJ_16410) - 1496798..1497817 (+) 1020 WP_104838389.1 M24 family metallopeptidase -
  MMJJ_RS08155 (MMJJ_16420) cbiD 1497857..1498954 (+) 1098 WP_104838390.1 cobalt-precorrin-5B (C(1))-methyltransferase CbiD -
  MMJJ_RS08160 (MMJJ_16430) tsaA 1498962..1499345 (+) 384 WP_244901573.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  MMJJ_RS08165 (MMJJ_16440) ecnA 1499355..1499912 (+) 558 WP_104838392.1 calcium-activated nuclease EcnA Unclear
  MMJJ_RS08170 (MMJJ_16450) lysA 1500165..1501475 (-) 1311 WP_104838393.1 diaminopimelate decarboxylase -
  MMJJ_RS08175 (MMJJ_16460) - 1501612..1502466 (-) 855 WP_104838394.1 phosphate signaling complex PhoU family protein -
  MMJJ_RS08180 (MMJJ_16470) - 1502570..1503334 (-) 765 WP_011171142.1 ABC transporter ATP-binding protein -
  MMJJ_RS08185 (MMJJ_16480) - 1503377..1504138 (-) 762 WP_104838395.1 ABC transporter permease -
  MMJJ_RS08190 (MMJJ_16490) - 1504321..1504869 (-) 549 WP_104838396.1 hypothetical protein -

Sequence

Protein


Download         Length: 185 a.a.        Molecular weight: 21636.91 Da        Isoelectric Point: 4.4987

>NTDB_id=68 MMJJ_RS08165 WP_104838392.1 1499355..1499912(+) (ecnA) [Methanococcus maripaludis strain DSM 2067]
MKKTCFLAPILFLIFSGCISSNNQNYDSKDFIDSHEHLFGTVTRVVDGDTVHVDSNGVDYNIRLLGVDTPETYQKNSVSE
YYLDYETPISDIEYLDYWGHLATNYTRDTLENKSVYVVFDKKSEKQDKYGRYLAYIYLENENFNENLIEYGFARVYTSNF
ELKNDFLDTEKTAKSGRIGLWNYNN

Nucleotide


Download         Length: 558 bp        

>NTDB_id=68 MMJJ_RS08165 WP_104838392.1 1499355..1499912(+) (ecnA) [Methanococcus maripaludis strain DSM 2067]
ATGAAAAAAACATGTTTTTTAGCCCCAATTTTATTTTTAATTTTTTCAGGTTGTATTTCAAGCAATAATCAGAATTATGA
TTCAAAAGATTTCATAGATTCTCACGAACATTTATTTGGAACAGTTACTCGAGTAGTTGATGGAGACACGGTCCACGTTG
ATTCAAACGGAGTTGATTATAATATCAGGCTTTTAGGGGTTGATACTCCAGAAACTTATCAAAAAAATAGTGTTTCAGAA
TATTATCTTGATTATGAAACTCCAATTTCAGATATTGAATATCTGGATTACTGGGGGCATTTGGCAACAAACTATACAAG
AGATACGCTCGAAAACAAATCAGTTTATGTAGTTTTTGATAAAAAAAGTGAAAAACAGGACAAATACGGAAGATATCTTG
CATATATTTATTTAGAAAATGAAAATTTCAATGAAAATCTGATAGAATATGGATTTGCAAGAGTATATACAAGCAATTTT
GAATTAAAGAATGATTTTTTAGATACTGAAAAAACAGCAAAATCGGGCAGAATCGGCCTTTGGAATTACAACAATTAA

Domains

Predicted by InterproScan.

Staphylococcal nuclease homologue (SNase)

(61-182)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L1CCC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Dallas R Fonseca et al. (2024) An Extracellular, Ca2+-Activated Nuclease (EcnA) Mediates Transformation in a Naturally Competent Archaeon. Molecular Microbiology 122(4):477-490. [PMID: 39214865]
[2] Dallas R Fonseca et al. (2023) Random transposon mutagenesis identifies genes essential for transformation in Methanococcus maripaludis. Molecular Genetics And Genomics : MGG 298(3):537-548. [PMID: 36823423]