Detailed information NTG0678990

Bioinformatically predicted

Overview

• Name: addB
• Type: Machinery gene
• Locus tag: MU858_RS05770
• Genome accession: NZ_CP095874
• Coordinates: 1109105..1112620 (+)
• Length: 1171 a.a.
• NCBI ID: WP_247998844.1
• Uniprot ID: -
• Organism: Bacillus sp. PGP15
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1104105..1117620

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MU858_RS05730 (MU858_05730)	cspA	1104535..1104738 (+)	204	WP_000301519.1	RNA chaperone/antiterminator CspA	-
MU858_RS05735 (MU858_05735)	-	1104857..1105018 (+)	162	Protein_1036	IS5/IS1182 family transposase	-
MU858_RS05740 (MU858_05740)	-	1105109..1105327 (-)	219	WP_216475462.1	hypothetical protein	-
MU858_RS05745 (MU858_05745)	-	1105564..1105749 (-)	186	WP_216475463.1	hypothetical protein	-
MU858_RS05750 (MU858_05750)	-	1106034..1106615 (+)	582	WP_216475464.1	competence protein ComK	-
MU858_RS05755 (MU858_05755)	-	1106857..1107453 (+)	597	WP_071758313.1	DUF4306 domain-containing protein	-
MU858_RS05760 (MU858_05760)	-	1107775..1108368 (+)	594	WP_048526648.1	TVP38/TMEM64 family protein	-
MU858_RS05765 (MU858_05765)	lepB	1108425..1108988 (+)	564	WP_048526650.1	signal peptidase I	-
MU858_RS05770 (MU858_05770)	addB	1109105..1112620 (+)	3516	WP_247998844.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
MU858_RS05775 (MU858_05775)	addA	1112617..1116342 (+)	3726	WP_247998845.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
MU858_RS05780 (MU858_05780)	-	1116355..1116642 (+)	288	WP_000255723.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
MU858_RS05785 (MU858_05785)	-	1116736..1117113 (-)	378	WP_247998846.1	DUF1433 domain-containing protein	-

Sequence

Protein

• Length: 1171 a.a.
• Molecular weight: 134370.41 Da
• Isoelectric Point: 5.1127

>NTDB_id=678990 MU858_RS05770 WP_247998844.1 1109105..1112620(+) (addB) [Bacillus sp. PGP15]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGETILYLVPEQMTFQTQQALISSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVNGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTLDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFYSQSLAHLETHYEARPNEKFHGEASVTISTAANL
RAEVEGVAREIRRLVADEDYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTDGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDRSRLSNVKATFVIGVNEGVIPAAPMDEGMLSDEERDVLSAAGIEL
APTTRQTLLEEQFVMYQMVTRATEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKDTFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTVVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSVLVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TYLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGGTASPAGVLYFHIHNPIVEMKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide

• Length: 3516 bp

>NTDB_id=678990 MU858_RS05770 WP_247998844.1 1109105..1112620(+) (addB) [Bacillus sp. PGP15]
ATGTCACTTCGATTTGTAATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTAAA
GCAGCGTCCAAGAGGGGAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAGCAGGCATTAATTAGTA
GTGAGGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAGGTGCTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCACATTGATGAAGCAGGCGTACATATGTTACTTCGTAAAATTGTAGAATCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTTTTTGAACATCTTGGTAGTATGATTGCGGAGTTTAAACGTTACA
ACGTGACGCCATCTAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCCGAACAAAAGCTATTAGCG
AATAAAGTGTATGATTTACAACTACTATATGATGATTTCGAGCGTGCTTTAATCGGAAAATATTTAGATTCAGAAGATTA
CTTGCAATTGCTAGTAGAAAAGCTTCCGCAGTCTGAATATGTAAATGGGGCTGAAATTTATATAGATGGATTTCATTCAT
TCTCACCGCAAGAACTAGAAATTGTAAGACAGCTTATGATTTGCGGTGCGAGAGTGACAATCACGTTAACACTAGATGAA
AAAACGTTAGCACAGCCAGTAAATGAACTAGATTTATTTTATGAAACGACGTTAACATATGAAAAAATAAAACAAGTAGC
ACGTGAAGAGAAGATAGAAATTGAAAAAACGATTCCGCTTATGGAGCAACCGCGTTTTTATTCGCAATCATTAGCGCATT
TAGAAACGCATTACGAAGCTCGTCCAAATGAAAAGTTTCACGGAGAAGCGAGTGTAACGATTAGTACAGCAGCGAATTTA
CGAGCTGAAGTAGAAGGCGTTGCGCGTGAAATTCGTAGACTTGTGGCAGATGAAGACTATCGTTACCGAGATATCGCGGT
ACTTCTTCGTAACGGGGAAAGTTATTATGATGTAATGCGAACGCTATTTACAGATTATAATATCCCGCACTTCATTGATG
AAAAGCGCCCGATGTCACATCATCCGCTAGTAGAATGCATTCGTTCTGCACTCGAGATTATTAGCGGGAATTGGCGTTAT
GATGCAGTGTTTCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAACGATGCGCGAAGAGATGGA
TGAGTTTGAAAACTACTGTTTAGCTTATGGTGTACAAGGGAAGAGATGGACTTCTGAGGATCCGTGGATGTATCGCCGCT
ATCGTTCTCTTGACGATACGGACGGGATGATTACAGACAGTGAACGTGAAATGGAAGAGAAAATAAATCGATTGCGTGAC
GTTGTAAGAACGCCTGTTATTCGTATGCAAAAAAGACTGAAGCGTGCGGGAACAGTTATGCAAATGTGCGAAGCTGTTTA
CTTATTTTTAGAAGAGCTTGACGTTCCGAAAAAGCTAGAAGCATTACGTATTCGTGCAGAAGAGAGCGGAGATTTCTTAT
TCGCGACAGATCACGAGCAAGTATGGGAAGAAGTAATGAGTCTTCTTGATACGTTCGTAGAGATGCTTGGCGAAGAGAAA
ATGTCACTTTCTATGTTCACAGACGTTATGTCGACAGGTCTTGAGGCGCTTCAATTCGCCAACATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCGAATATTGATCGTTCAAGATTATCAAATGTGAAAGCAACATTTGTCATTGGCGTAAATGAAGGAG
TTATTCCAGCAGCACCGATGGATGAAGGTATGCTTTCTGATGAGGAAAGAGACGTTCTTAGTGCTGCAGGTATCGAATTA
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTCGTTATGTACCAAATGGTAACGAGAGCAACTGAGAAATTATA
CATTTCTTGTCCGCTTGCAGATGAGGAAGGAAAGACGTTACTTGCTTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGATACATTTATTACGAATGACGTAAATGATTTATCCCGTTCGGAACAAATTTCATACGTAGCAACGCCA
GAAGTAACGTTATCTTACGTTATGCAGCAACTTCAAACGTGGAAGCGATACGGATTTGAAGGGAATTTAGACTTTTGGTG
GGACGTATATAACTTCTACGTTACTTCAGATGAATGGAAGCAAAAAAGTAGCCGCGTATTATCAAGCTTATTCTACCGAA
ATCGTGCGCAAAAGCTAAGTACAGTTGTAAGTAGAGATTTATACGGAGATAAAATAAAAGGAAGCGTCTCTCGTATGGAA
CTATTTAACCGTTGCGCATACGCTCATTTCGCGCAGCACGGTTTATCGTTAAGAGAGCGTGATATTTTTAAACTGGATGC
GCCAGATATCGGCGAATTATTCCATGCAGCGCTGAAGAGAATTGCAGACAGGCTATTACGTGAAAATCGTACTTGGGCAG
ACTTATCAATAAAAGAATGCGAGCATCTTTCTGTTTTAGTAATAGAAGAGATTGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCAAACCGCCATTTCTATTTAAAACAAAAATTGCAGCAAATCATTTTCCGTACGTCAATCATTCTACGTGAACA
TGCGAAATCAAGTGGTTTCGTACCAGTAGATTTAGAAGTACCGTTCGGTATGGGCGGTACAGGATCGCTTCCGCCGATGG
AATTCTCATTACCAAATGGTGTGAAGATGGAAGTAGTTGGCCGTATTGACCGTGTTGATAAGGCAGAAGATGAAAACGGG
ACATACCTCCGTATTATTGATTATAAATCGAGCTCAAAAGCGTTAGATTTAACGGAAGTATACTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACATACGTGGATGAAAAAAGGCGGCACGGCTTCACCGGCTGGTG
TACTGTACTTCCATATCCATAACCCAATCGTTGAGATGAAAGGTGACGCATCTGAAGCGGAAATTGAGAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGACTCGTGCTAGGAGATGCTGACGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
CTCTGATATTATTTCTGCTGGTCTGAAAAAAGACGGTAGCTTTAGTGCGCGTTCAAGCATTGCAAGTGAACAAGAGTTTA
ACGTACTGCAAAAATATGTACACCATACGTTTGAAAATATCGGAAAAGATATTACAGAAGGCGTGATCGATATTGCCCCT
TATAAAAAAGGGAATAAAGCGGCGTGTACGTTCTGTAACTTCAAATCTGTTTGTCAGTTCGATGAATCACTTGAAGATAA
CCAATTCCGTACGCTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (795-1139)

• UvrD-like helicase C-terminal domain (UvrD_C) (303-669)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addB	Bacillus subtilis subsp. subtilis str. 168	49.061	100	0.491