Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   H7R25_RS02000 Genome accession   NZ_AP022261
Coordinates   448325..448792 (-) Length   155 a.a.
NCBI ID   WP_000338336.1    Uniprot ID   -
Organism   Escherichia coli strain WP8-S18-ESBL-07     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 443325..453792
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R25_RS01975 (WP8S18E07_03950) rpsG 443466..443936 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  H7R25_RS01980 (WP8S18E07_03960) fusA 444033..446147 (+) 2115 WP_000124703.1 elongation factor G -
  H7R25_RS01985 (WP8S18E07_03970) tuf 446218..447402 (+) 1185 WP_000031783.1 elongation factor Tu -
  H7R25_RS01990 (WP8S18E07_03980) bfd 447585..447779 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  H7R25_RS01995 (WP8S18E07_03990) bfr 447852..448328 (+) 477 WP_000675515.1 bacterioferritin -
  H7R25_RS02000 (WP8S18E07_04000) pilD 448325..448792 (-) 468 WP_000338336.1 A24 family peptidase Machinery gene
  H7R25_RS02005 (WP8S18E07_04010) rpsJ 449171..449482 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  H7R25_RS02010 (WP8S18E07_04020) rplC 449515..450144 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  H7R25_RS02015 (WP8S18E07_04030) rplD 450155..450760 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  H7R25_RS02020 (WP8S18E07_04040) rplW 450757..451059 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  H7R25_RS02025 (WP8S18E07_04050) rplB 451077..451898 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  H7R25_RS02030 (WP8S18E07_04060) rpsS 451915..452193 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  H7R25_RS02035 (WP8S18E07_04070) rplV 452208..452540 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  H7R25_RS02040 (WP8S18E07_04080) rpsC 452558..453259 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  H7R25_RS02045 (WP8S18E07_04090) rplP 453272..453682 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 17034.17 Da        Isoelectric Point: 8.1255

>NTDB_id=67161 H7R25_RS02000 WP_000338336.1 448325..448792(-) (pilD) [Escherichia coli strain WP8-S18-ESBL-07]
MDAALPFLILYACLSTLLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=67161 H7R25_RS02000 WP_000338336.1 448325..448792(-) (pilD) [Escherichia coli strain WP8-S18-ESBL-07]
ATGGACGCTGCCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCAACCCTACTTTTTTTCTGGGATGCAAAGCATGGCTT
GTTACCTGACAGGTTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTATCAAGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGCCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACATGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTATTGGTTTGCTTATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGTCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGCTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.882

81.935

0.368

Multiple sequence alignment