Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   MQW34_RS05780 Genome accession   NZ_CP094455
Coordinates   1122704..1126219 (+) Length   1171 a.a.
NCBI ID   WP_243499600.1    Uniprot ID   -
Organism   Bacillus sp. ZJS3     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1117704..1131219
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MQW34_RS05745 (MQW34_05740) - 1117913..1118704 (-) 792 WP_243499598.1 phosphotransferase -
  MQW34_RS05750 (MQW34_05745) cspA 1119056..1119259 (+) 204 WP_000301518.1 RNA chaperone/antiterminator CspA -
  MQW34_RS05755 (MQW34_05750) - 1119450..1119662 (-) 213 WP_236797621.1 hypothetical protein -
  MQW34_RS05760 (MQW34_05755) - 1119979..1120164 (-) 186 WP_000391382.1 hypothetical protein -
  MQW34_RS05765 (MQW34_05760) - 1120446..1121027 (+) 582 WP_202321784.1 competence protein ComK -
  MQW34_RS05770 (MQW34_05765) - 1121374..1121967 (+) 594 WP_000347517.1 TVP38/TMEM64 family protein -
  MQW34_RS05775 (MQW34_05770) lepB 1122024..1122587 (+) 564 WP_071723866.1 signal peptidase I -
  MQW34_RS05780 (MQW34_05775) addB 1122704..1126219 (+) 3516 WP_243499600.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  MQW34_RS05785 (MQW34_05780) addA 1126216..1129941 (+) 3726 WP_243499602.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  MQW34_RS05790 (MQW34_05785) - 1129954..1130241 (+) 288 WP_000179960.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  MQW34_RS05795 (MQW34_05790) gerPF 1130283..1130498 (-) 216 WP_001141566.1 spore germination protein GerPF -
  MQW34_RS05800 (MQW34_05795) - 1130541..1130927 (-) 387 WP_000902314.1 spore germination protein GerPE -
  MQW34_RS05805 (MQW34_05800) gerPD 1130943..1131137 (-) 195 WP_001052802.1 spore germination protein GerPD -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134277.32 Da        Isoelectric Point: 5.1777

>NTDB_id=669629 MQW34_RS05780 WP_243499600.1 1122704..1126219(+) (addB) [Bacillus sp. ZJS3]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGETILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVNGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTLDE
KTLAQPVNELDLFYETTLTYEKIKQIAREEKIEIEKTIPLMEQPRFHSQSLAHLETHYEARPNEKFHGEASVTIRTAANL
RAEVEGVAREIRRLVADEDYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTDGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDRSRLSNVKATFVIGVNEGVIPAAPMDEGMLSDEERDVLSAAGIEL
APTTRQTLLEEQFVMYQMVTRATEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKDTFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSAVVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSAVVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGGTASPAGVLYFHIHNPIVEVKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=669629 MQW34_RS05780 WP_243499600.1 1122704..1126219(+) (addB) [Bacillus sp. ZJS3]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTAAA
GCAACGTCCAAGAGGGGAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAGCAGGCGTTAATTGGTA
GTGAAGATGTTAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGGTTAGCATGGAAGGTACTGCAAGAAGTTGGC
GGAGCGAGTCGCCTTCATATTGATGAAGCGGGCGTACATATGTTACTTCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTTTTTGAACATCTTGGTAGTATGATTGCGGAGTTTAAACGTTACA
ACGTGACGCCATCTAACGTATATGAAATGTGGCAACAATTAGATGCACATAGTAGCAGTGCCGAACAAAAGTTACTGGCG
AATAAAGTGTATGATTTACAATTACTATATGATGATTTTGAGCGTGCTTTAATCGGAAAATATTTAGATTCAGAAGACTA
TTTGCAATTACTAGTCGAAAAGCTTCCGCAGTCTGAATATGTAAATGGAGCTGAAATTTATATAGATGGATTTCATTCCT
TCTCACCGCAAGAACTAGAAATTGTAAGACAGCTTATGATTTGCGGTGCGAGAGTGACAATCACGTTAACGCTAGATGAA
AAAACGTTAGCACAGCCAGTAAATGAACTAGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAACAAATAGC
ACGTGAAGAGAAGATAGAAATTGAAAAAACGATTCCGCTTATGGAACAACCGCGTTTTCATTCGCAATCATTAGCACATT
TAGAAACGCATTATGAAGCACGACCAAATGAAAAGTTTCACGGAGAAGCAAGTGTAACGATTCGTACAGCAGCGAATTTA
CGAGCTGAAGTAGAAGGCGTTGCGCGTGAAATTCGTAGACTTGTGGCAGATGAAGACTATCGTTACCGAGATATCGCGGT
TCTTCTTCGTAACGGGGAAAGTTATTACGATGTGATGCGAACGCTATTTACAGATTATAATATCCCGCATTTCATCGATG
AAAAACGCCCGATGTCACATCATCCGCTAGTAGAATGCATTCGTTCTGCACTCGAGATTATTAGCGGGAATTGGCGTTAT
GATGCAGTGTTTCGCTGTGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAACGATGCGCGAAGAGATGGA
TGAGTTTGAAAACTACTGTTTAGCGTACGGTGTACAAGGAAAGAGATGGACTTCTGAAGATCCGTGGATGTACCGCCGCT
ATCGTTCTCTTGACGATACGGACGGGATGATTACAGACAGTGAACGTGAAATGGAAGAGAAAATAAATAGGTTACGCGAC
GTTGTAAGAACGCCTGTTATTCGTATGCAAAAAAGGCTGAAGCGTGCGGGAACAGTTATGCAAATGTGTGAAGCTGTTTA
CTTATTTTTAGAAGAACTGGACGTTCCGAAAAAACTAGAAGCATTACGTATTCGTGCAGAAGAAAGCGGAGATTTCTTAT
TCGCGACAGACCATGAGCAAGTATGGGAAGAAGTAATGAGCCTTCTTGATACGTTCGTTGAGATGCTTGGCGAAGAGAAA
ATGTCACTTTCTATGTTCACAGACGTCATGTCGACAGGTCTTGAGGCGCTTCAATTCGCAAACATTCCACCTTCATTAGA
CCAAGTGTTAATTGCGAATATTGATCGTTCAAGATTATCAAATGTGAAAGCAACATTTGTTATTGGCGTAAATGAAGGTG
TCATTCCAGCGGCACCGATGGATGAAGGGATGCTTTCTGATGAGGAAAGAGACGTTCTTAGTGCAGCAGGTATCGAACTC
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTCGTTATGTACCAAATGGTAACGAGAGCAACTGAAAAATTATA
TATTTCATGTCCACTAGCAGATGAAGAAGGAAAGACGTTACTTGCTTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGATACATTTATTACGAACGACGTAAATGATTTATCCCGTTCGGAACAAATTTCATACGTAGCAACGCCG
GAAGTAACGTTGTCATATGTTATGCAGCAACTACAGACGTGGAAGCGATATGGATTTGAAGGGAATTTAGACTTTTGGTG
GGATGTATATAATTTCTATGTAACTTCAGATGAATGGAAGCAAAAAAGTAGCCGCGTATTATCAAGCTTATTCTACCGAA
ATCGTGCGCAAAAGCTAAGTGCAGTTGTAAGTAGAGATTTATACGGAGACAAAATAAAAGGAAGCGTCTCTCGTATGGAA
CTATTTAACCGTTGTGCGTACGCTCATTTCGCGCAGCACGGTTTATCATTAAGAGAGCGTGATATTTTTAAACTTGATGC
GCCAGATATCGGTGAATTATTCCATGCAGCGCTGAAGAGAATTGCAGACAGGCTATTACGTGAAAATCGTACTTGGGCGG
ATTTATCAATAAAAGAATGTGAGCATCTTTCTGCTGTAGTAATAGAAGAAATTGCACCGTTATTACAAAGACAAATATTA
TTAAGTTCAAATCGTCATTTCTATTTAAAACAAAAACTACAACAAATTATTTTCCGTACGTCAATCATTCTTCGTGAGCA
CGCGAAATCGAGTGGTTTCGTACCAGTTGATTTAGAAGTACCATTCGGTATGGGCGGTACAGGATCGCTTCCGCCAATGG
AATTCTCGTTACCTAATGGTGTAAAGATGGAAGTAGTTGGCCGTATTGACCGCGTTGATAAGGCAGAAGATGAAAACGGA
ACATTCCTACGTATTATTGACTATAAATCAAGTTCAAAAGCGTTAGACTTAACGGAAGTGTATTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACATACGTGGATGAAAAAAGGCGGCACGGCATCACCAGCTGGTG
TACTGTACTTCCACATTCATAACCCGATTGTTGAGGTGAAAGGTGACGCATCTGAAGCAGAAATTGAAAAGGAAATTTTA
AAGAAATTTAAGATGAAAGGTCTCGTACTAGGAGATGCTGACGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGGAG
CTCTGATATTATTTCTGCTGGTCTGAAAAAAGACGGTAGCTTTAGTGCGCGTTCAAGTATTGCCAGTGAACAAGAGTTTA
ACGTCCTGCAAAAATACGTACACCATACGTTTGAAAATATCGGAAAAGACATTACAGAGGGTGTTATCGATATTGCTCCT
TACAAAAAGGGAAATAAAGCGGCATGTACGTTCTGTAACTTCAAATCTGTTTGTCAGTTCGATGAATCACTTGAAGATAA
CCAATTCCGTACGCTAAAAGATATGAAAGATAGTGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.148

100

0.493

Multiple sequence alignment