Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MQW34_RS00530 Genome accession   NZ_CP094455
Coordinates   97683..99059 (+) Length   458 a.a.
NCBI ID   WP_001085207.1    Uniprot ID   A0A2B4FB19
Organism   Bacillus sp. ZJS3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 92683..104059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MQW34_RS00510 (MQW34_00510) ctsR 92876..93337 (+) 462 WP_001244563.1 transcriptional regulator CtsR -
  MQW34_RS00515 (MQW34_00515) - 93511..94059 (+) 549 WP_078418160.1 UvrB/UvrC motif-containing protein -
  MQW34_RS00520 (MQW34_00520) - 94064..95128 (+) 1065 WP_078418159.1 protein arginine kinase -
  MQW34_RS00525 (MQW34_00525) clpC 95151..97586 (+) 2436 WP_000971179.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  MQW34_RS00530 (MQW34_00530) radA 97683..99059 (+) 1377 WP_001085207.1 DNA repair protein RadA Machinery gene
  MQW34_RS00535 (MQW34_00535) disA 99063..100136 (+) 1074 WP_078418158.1 DNA integrity scanning diadenylate cyclase DisA -
  MQW34_RS00540 (MQW34_00540) - 100297..101406 (+) 1110 WP_016089677.1 PIN/TRAM domain-containing protein -
  MQW34_RS00545 (MQW34_00545) ispD 101423..102103 (+) 681 WP_210626126.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MQW34_RS00550 (MQW34_00550) ispF 102219..102695 (+) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49814.39 Da        Isoelectric Point: 7.1421

>NTDB_id=669623 MQW34_RS00530 WP_001085207.1 97683..99059(+) (radA) [Bacillus sp. ZJS3]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQSEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIA
AHIEEVNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=669623 MQW34_RS00530 WP_001085207.1 97683..99059(+) (radA) [Bacillus sp. ZJS3]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGGTATCAGTCACCGAAATATATGGGGAAATGTCCTGGATG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCGGTTGTATCATCAAGGCGCCTTAATTATGCCAATGCAATTCAAT
CGGAAGTAACAAAACCAAGACGCCTAACAGAAGTAGAAACAAAGTCTGAGGCGCGTATTGAAACGAAATTTCAAGAATTT
AATCGTGTACTTGGTGGCGGGATTGTAGATGGTTCCTTAGTACTTATTGGTGGAGACCCTGGGATTGGAAAATCAACATT
ACTATTACAAATATCATCGCAATTAGCAGATTCTTCATATGATGTACTATATATATCAGGTGAAGAATCAGCAAAACAGA
TTAAACTGCGCGCGGATCGTTTACATGTAAATGGTAGTAATCTATTTGTTGTATCAGAGACGGATTTACAGCGAATTGCA
GCACACATTGAAGAAGTGAATCCAGCCTTTGTTGTTATTGATTCCATCCAAACGATACATTTACCTGAGGTAACGTCAGC
ACCGGGAAGTGTGGCACAAGTACGTGAATGTACAGCGGAATTAATGAAACTTGCAAAAACGAAAGGGATCCCTATTTTTA
TCGTTGGACATGTGACAAAAGAGGGTGCAATCGCAGGACCTCGTATGTTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGACCGTCATCATACATATCGTATTTTGCGAGCTGTGAAGAATCGTTTTGGTTCTACAAATGAAATGGGTATTTT
TGAAATGAAGGAACTTGGTCTTGCGGAAGTCTTAAACCCTTCTGAAATTTTCCTTGAGGAAAGACCAGTTGGGGTTGCAG
GATCAACAGTGGTTGCCTCAATGGAAGGAACACGACCAGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAACCCTCGAAGAATGGCAACGGGAATTGACCATAACCGTGTATCGCTTATTATGGCAGTGTTAGAAAAAAGAACAGG
TTTATTATTGCAAAATCAAGACGCATATTTAAAAGTAGCAGGTGGTTTGAAGTTAGATGAACCGGCAATTGATTTAGCTG
TGGCTTTAAGTATAGCTTCAAGTTTTAGAGATAAATCTACGGCACCGACCGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGGGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCTAAATTAGGATTTCAACGCGCTATTATTCC
TAGAAAGAATTTAGGAGGATGGACAATTCCGGATGGGATTGAGGTAGTAGGTGTATCCAATTTAGGAGAAGCACTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-222)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2B4FB19

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.728

100

0.749
  radA Streptococcus pneumoniae Rx1

63.797

98.908

0.631
  radA Streptococcus pneumoniae D39

63.797

98.908

0.631
  radA Streptococcus pneumoniae R6

63.797

98.908

0.631
  radA Streptococcus pneumoniae TIGR4

63.797

98.908

0.631
  radA Streptococcus mitis SK321

63.797

98.908

0.631
  radA Streptococcus mitis NCTC 12261

63.576

98.908

0.629

Multiple sequence alignment