Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   MON12_RS24135 Genome accession   NZ_CP093424
Coordinates   4643771..4647286 (-) Length   1171 a.a.
NCBI ID   WP_074568010.1    Uniprot ID   A0A1Y6A173
Organism   Bacillus pacificus strain MP6     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4638771..4652286
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MON12_RS24110 (MON12_24110) gerPD 4638814..4639008 (+) 195 WP_001102341.1 spore germination protein GerPD -
  MON12_RS24115 (MON12_24115) - 4639024..4639410 (+) 387 WP_000902317.1 spore germination protein GerPE -
  MON12_RS24120 (MON12_24120) gerPF 4639453..4639668 (+) 216 WP_001141566.1 spore germination protein GerPF -
  MON12_RS24125 (MON12_24125) - 4639749..4640036 (-) 288 WP_000179961.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  MON12_RS24130 (MON12_24130) addA 4640049..4643774 (-) 3726 WP_000970455.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  MON12_RS24135 (MON12_24135) addB 4643771..4647286 (-) 3516 WP_074568010.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  MON12_RS24140 (MON12_24140) lepB 4647403..4647966 (-) 564 WP_000751900.1 signal peptidase I -
  MON12_RS24145 (MON12_24145) - 4648024..4648617 (-) 594 WP_000347516.1 TVP38/TMEM64 family protein -
  MON12_RS24150 (MON12_24150) - 4648924..4649520 (-) 597 WP_074568009.1 DUF4306 domain-containing protein -
  MON12_RS24155 (MON12_24155) - 4649733..4650314 (-) 582 WP_088106549.1 competence protein ComK -
  MON12_RS24160 (MON12_24160) - 4650597..4650782 (+) 186 WP_074567789.1 hypothetical protein -
  MON12_RS24165 (MON12_24165) - 4651091..4651309 (+) 219 WP_070805466.1 hypothetical protein -
  MON12_RS24170 (MON12_24170) cspA 4651646..4651849 (-) 204 WP_000301518.1 RNA chaperone/antiterminator CspA -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134185.31 Da        Isoelectric Point: 5.2121

>NTDB_id=664642 MON12_RS24135 WP_074568010.1 4643771..4647286(-) (addB) [Bacillus pacificus strain MP6]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGETILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVKGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTLDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLEAHYEARPNEKFNGEASVTIHTAANL
RAEVEGVAREIRRLVADEDYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTNGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDRSRLSNVKATFVIGVNEGVIPAAPMDEGMLSDEERDVLSAAGIEL
APTTRQTLLEEQFVMYQMVTRATEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKDTFITNDVNDLSRLEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTAVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSAVVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGGTASPAGVLYFHIHNPIVEVKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=664642 MON12_RS24135 WP_074568010.1 4643771..4647286(-) (addB) [Bacillus pacificus strain MP6]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTAAA
GCAGCGCCCAAGAGGGGAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAGCAGGCGTTAATTGGTA
GTGAGGATGTTAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCTTGGAAAGTATTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCACATTGATGAAGCGGGCGTACATATGTTACTTCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTATTCCAAAAAGCAGCGGAGCAAAATGGTTTCTTTGAACATCTTGGCAGTATGATTGCGGAGTTTAAACGATATA
ACGTGACGCCATCAAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCCGAACAAAAGCTATTAGCG
AATAAAGTGTATGATTTACAGTTATTATATGATGATTTTGAACGTGCTTTAATCGGAAAGTACTTAGATTCAGAAGACTA
TCTGCAATTGCTAGTGGAAAAGCTTCCGCAGTCTGAATATGTAAAGGGAGCTGAAATTTATATAGATGGCTTCCATTCGT
TTTCACCGCAAGAACTAGAAATTGTAAGACAGCTTATGATTTGCGGAGCGAGAGTGACAATCACGTTAACGCTAGATGAA
AAAACGTTAGCGCAGCCAGTAAATGAACTAGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAACAAGTAGC
GCGTGAAGAGAAGATAGAAATTGAAAAAACGATTCCACTTATGGAACAGCCGCGTTTTCATTCTCCGGCGTTAGCGCATT
TAGAAGCTCATTATGAAGCGCGTCCGAATGAGAAATTTAACGGGGAAGCAAGTGTAACGATTCATACAGCCGCGAATTTA
CGAGCGGAAGTAGAAGGCGTTGCTCGTGAAATTCGTAGACTTGTGGCAGATGAAGACTATCGTTACCGAGATATTGCAGT
TCTTCTTCGTAACGGAGAAAGTTATTACGATGTAATGCGGACTCTATTTACAGATTATAATATCCCGCACTTTATCGATG
AAAAACGCCCGATGTCACATCATCCGTTAGTAGAATGCATTCGTTCTGCACTCGAGATTATTAGCGGGAATTGGCGTTAT
GATGCAGTATTTCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAACGATGCGCGAAGAGATGGA
TGAGTTTGAAAACTACTGTTTAGCATACGGTGTACAAGGGAAAAGATGGACTTCTGAGGATCCATGGATGTATCGCCGCT
ATCGTTCTCTTGACGATACGAACGGGATGATTACAGACAGCGAACGTGAAATGGAAGAGAAAATAAATCGATTGCGTGAC
GTTGTAAGAACGCCCGTTATTCGTATGCAAAAAAGACTGAAGCGTGCGGGAACGGTTATGCAAATGTGCGAAGCTGTTTA
CTTATTTTTAGAAGAGCTTGACGTTCCGAAAAAATTAGAAGCATTACGTATTCGTGCAGAAGAGAGCGGAGATTTCTTAT
TTGCGACAGATCATGAACAAGTATGGGAAGAAGTAATGAGTCTTCTTGATACGTTTGTTGAGATGCTTGGCGAAGAAAAA
ATGTCACTTTCTATGTTCACAGACGTTATGTCGACAGGTCTTGAGGCGCTTCAATTCGCAAACATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCGAATATTGATCGTTCTAGATTATCAAATGTGAAAGCAACATTCGTCATTGGAGTGAATGAAGGTG
TGATTCCAGCAGCACCGATGGATGAAGGTATGCTTTCTGATGAAGAAAGAGATGTTCTTAGCGCTGCAGGTATTGAACTC
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTTGTTATGTACCAAATGGTAACGAGAGCAACTGAGAAATTATA
CATTTCATGCCCGCTTGCAGATGAGGAAGGAAAGACGTTACTTGCTTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGATACATTTATTACGAACGACGTAAATGATTTATCACGTTTGGAACAAATTTCATATGTAGCAACACCA
GAAGTAACGTTGTCATATGTTATGCAGCAACTTCAAACATGGAAGCGATATGGATTTGAAGGCAACTTAGACTTTTGGTG
GGATGTATATAATTTCTACGTTACTTCAGATGAATGGAAGCAAAAAAGTAGCCGCGTATTATCAAGCTTATTCTACCGAA
ATCGTGCGCAAAAGCTAAGTACAGCTGTAAGTAGAGATTTATATGGAGACAAAATAAAAGGAAGCGTTTCTCGTATGGAA
TTATTTAACCGTTGTGCGTACGCTCATTTTGCGCAGCACGGTTTATCGTTAAGAGAGCGTGATATTTTCAAACTGGATGC
GCCAGATATTGGGGAGCTATTCCATGCAGCACTGAAGAGAATTGCGGACAGGCTATTACGTGAAAACCGTACTTGGGCGG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCTGCTGTAGTAATAGAAGAAATTGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACGTCAATCATTCTTCGTGAACA
TGCGAAGTCTAGCGGTTTCGTACCAGTTGATTTAGAAGTGCCATTTGGTATGGGCGGTACAGGATCGCTTCCGCCAATGG
AATTCTCATTACCAAATGGTGTGAAGATGGAAGTAGTTGGCCGTATTGACCGTGTTGATAAGGCAGAAGATGAAAACGGA
ACATTCCTACGTATTATTGACTATAAATCAAGCTCAAAAGCGTTAGACTTAACGGAAGTGTATTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCGCATACGTGGATGAAAAAAGGCGGCACGGCATCACCAGCTGGTG
TATTGTATTTCCACATTCATAACCCAATTGTGGAGGTAAAAGGTGACGCATCTGAAGCAGAAATTGAAAAGGAAATTTTA
AAGAAATTCAAAATGAAAGGTCTCGTACTAGGAGATGCTGACGTTGTTCGTTTAATGGATAATAAACTTTCAACAGGAAG
CTCTGATATTATTTCTGCTGGTCTGAAAAAAGACGGTAGTTTTAGTGCGCGTTCAAGTATTGCAAGTGAACAAGAGTTTA
ACGTCCTGCAAAAATACGTACACCATACGTTTGAAAATATCGGAAAAGACATTACAGAAGGCGTTATCGATATTGCTCCT
TACAAAAAGGGGAATAAAGCGGCGTGTACGTTCTGTAACTTCAAATCTGTTTGCCAGTTCGATGAATCACTTGAAGATAA
CCAGTTCCGTACGTTAAAAGATATGAAAGATAGTGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Y6A173

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.232

100

0.493