Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MKW86_RS03195 Genome accession   NZ_CP092906
Coordinates   676368..677402 (-) Length   344 a.a.
NCBI ID   WP_011707389.1    Uniprot ID   A0A081UZ58
Organism   Aeromonas hydrophila strain LP0103     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 671368..682402
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKW86_RS03175 (MKW86_03175) - 672186..672896 (-) 711 WP_241417262.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  MKW86_RS03180 (MKW86_03180) srmB 673025..674248 (+) 1224 WP_016351974.1 ATP-dependent RNA helicase SrmB -
  MKW86_RS03185 (MKW86_03185) yaaA 674425..675198 (-) 774 WP_241417263.1 peroxide stress protein YaaA -
  MKW86_RS03190 (MKW86_03190) pilU 675230..676339 (-) 1110 WP_017411211.1 type IVa pilus ATPase TapU Machinery gene
  MKW86_RS03195 (MKW86_03195) pilT 676368..677402 (-) 1035 WP_011707389.1 type IVa pilus ATPase TapT Machinery gene
  MKW86_RS03200 (MKW86_03200) - 677442..678143 (+) 702 WP_241417264.1 YggS family pyridoxal phosphate-dependent enzyme -
  MKW86_RS03205 (MKW86_03205) proC 678202..679026 (+) 825 WP_101615563.1 pyrroline-5-carboxylate reductase -
  MKW86_RS03210 (MKW86_03210) - 679067..679618 (+) 552 WP_241417265.1 YggT family protein -
  MKW86_RS03215 (MKW86_03215) yggU 679618..679917 (+) 300 WP_011707385.1 DUF167 family protein YggU -
  MKW86_RS03220 (MKW86_03220) - 679936..680355 (+) 420 WP_011707384.1 DUF4426 domain-containing protein -
  MKW86_RS03225 (MKW86_03225) - 680404..680715 (-) 312 WP_241417266.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38287.06 Da        Isoelectric Point: 6.7439

>NTDB_id=661027 MKW86_RS03195 WP_011707389.1 676368..677402(-) (pilT) [Aeromonas hydrophila strain LP0103]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEYPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVAALDAKAKAVDPNTI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=661027 MKW86_RS03195 WP_011707389.1 676368..677402(-) (pilT) [Aeromonas hydrophila strain LP0103]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAACATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGCGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACCGGGAAGTGCACGCCCTCATCTACGACA
TCATGAACGACCACCAGCGCAAGGAACTGGAAGAGAACTTCGAGGTGGACTTCTCGTTCGAGGTGCCGAACCTCGCCCGC
TTCCGGGTCAACGCCTTCCAGCAGGCGCGCGGCTCCGGTGCGGTATTTCGGACCATCCCCAGCACGGTGCTGAGCCTCGA
AGATCTGGACGCGCCGGAGATCTTTCGCAAGATTGCCGAATACCCGCGCGGCCTGGTGCTGGTGACCGGTCCGACCGGCT
CGGGTAAATCCACCACCCTGGCGGCCATGGTCAACTACATCAACGAGAACTTTCATCACCACATCCTCACCATCGAGGAT
CCCATCGAATTCGTGCACGAAAACAAGCGCTGCCTGGTGAACCAGCGGGAAGTGCACCGCGATACCAAGAGCTTCAGCAA
CGCTCTGCGCTCGGCGCTGCGGGAAGACCCGGACATCATCCTGGTGGGCGAGATGCGCGATCTGGAAACCATTCGTCTGG
CCATGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCATCGGCAGCCAAGACCATCGACCGGATC
ATCGACGTCTTCCCCGGTGCGGAAAAAGACATGGTGCGCTCCATGCTCTCCGAGTCCCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGCATCGGTGGCGGCCGGGTGGCGGCCCACGAGATCATGATGGGGATTCCCGCCGTGCGTAACCTCATCC
GGGAAGACAAGATTGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTGAGCCGCGGCGTAGTGGCGGCGCTGGATGCCAAGGCCAAGGCGGTCGATCCCAACACCATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A081UZ58

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Acinetobacter baylyi ADP1

77.326

100

0.773
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Pseudomonas stutzeri DSM 10701

76.833

99.128

0.762
  pilT Pseudomonas aeruginosa PAK

75.953

99.128

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.558

97.965

0.75
  pilT Vibrio cholerae strain A1552

76.558

97.965

0.75
  pilT Legionella pneumophila strain Lp02

72.647

98.837

0.718
  pilT Legionella pneumophila strain ERS1305867

72.647

98.837

0.718
  pilT Neisseria meningitidis 8013

70.796

98.547

0.698
  pilT Neisseria gonorrhoeae MS11

70.501

98.547

0.695
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407