Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   MLD62_RS22560 Genome accession   NZ_CP092833
Coordinates   4409188..4411509 (-) Length   773 a.a.
NCBI ID   WP_241138629.1    Uniprot ID   -
Organism   Bacillus mycoides strain LX30     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4404188..4416509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MLD62_RS22540 - 4405414..4405590 (-) 177 WP_214931467.1 hypothetical protein -
  MLD62_RS22545 - 4405757..4406197 (-) 441 WP_002111994.1 D-tyrosyl-tRNA(Tyr) deacylase -
  MLD62_RS22550 relA 4406215..4408398 (-) 2184 WP_085312421.1 GTP diphosphokinase -
  MLD62_RS22555 - 4408610..4409122 (-) 513 WP_002015305.1 adenine phosphoribosyltransferase -
  MLD62_RS22560 recJ 4409188..4411509 (-) 2322 WP_241138629.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  MLD62_RS22565 - 4411611..4412504 (-) 894 WP_002015308.1 cation diffusion facilitator family transporter -
  MLD62_RS22570 secDF 4412812..4415076 (-) 2265 WP_137056992.1 protein translocase subunit SecDF -
  MLD62_RS22575 - 4415358..4415642 (-) 285 WP_002143227.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 773 a.a.        Molecular weight: 87293.72 Da        Isoelectric Point: 4.9620

>NTDB_id=660728 MLD62_RS22560 WP_241138629.1 4409188..4411509(-) (recJ) [Bacillus mycoides strain LX30]
MLQPKTRWKEKEYNEELVSELANKLQLSPLVTSLFLGRGLNTEDKIVDFLNTADQEFHDPFLLEGMDRTVERVKKAIENG
EQILIFGDYDADGVSSTTVLYLALKELGAEVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEAKVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLEGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VQRGLKHMRMTRNIGLKALFKVANVSQSEITEESIGFSLAPRINAVGRLEDATPAVHLLLSEDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVENNFPPEENKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLSIDPHKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLNMNDVDELRRRLNEQADVILTAEDFIPITAVDAFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDADLESIRAIGSDGSHLKMALRDGQATLDTIGFGFGTYAKEISPVAKVSVVGEVSINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNVEANLAELPKEKITMVYFSEEVLNKFSLEGYKEQLVHASEVAHFDDQYIVLLDLPKGTDELGD
VFKAGFPARIYTLFYQENNHLFSTVPTREHFKWYYSFLSQKKPFSLRQYGEQLCRHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRDHK

Nucleotide


Download         Length: 2322 bp        

>NTDB_id=660728 MLD62_RS22560 WP_241138629.1 4409188..4411509(-) (recJ) [Bacillus mycoides strain LX30]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATGAGGAACTAGTGAGTGAATTAGCAAATAAATTGCAACT
ATCACCTCTTGTGACTTCATTATTTCTTGGCAGAGGCTTAAATACAGAAGATAAGATTGTAGACTTTTTAAATACAGCAG
ATCAAGAATTTCATGATCCATTTTTACTTGAAGGAATGGATCGGACTGTAGAACGTGTGAAAAAAGCTATTGAAAATGGA
GAACAAATATTAATATTTGGCGATTACGATGCGGACGGAGTAAGTAGTACGACTGTGTTATATCTTGCTCTCAAAGAATT
AGGTGCAGAAGTAGAGTTTTATATTCCAAATCGTTTTACTGAAGGCTATGGGCCAAATGAAGAAGCGTTTCGTTGGGCGC
ACAGTGCAGGGTTCTCACTAATTATTACTGTCGATACTGGTATCGCAGCGGTACATGAAGCGAAGGTAGCGAAGGAACTT
GGAATAGATCTTATTATTACAGATCATCATGAGCCACCACCAGAATTACCAGAGGCACTTGCGATTATTCACCCGAAATT
AGAAGGCGGTGTTTATCCGTTTCATTATTTAGCGGGTGTTGGTGTTGCATTTAAAGTTGCACATGCACTTTTGGGCCGTG
TGCCAGAGCATTTGTTGGAAATTGCAGTAATCGGTACGGTAGCCGATTTAGTATCGCTTCACGGTGAAAATAGATTATTA
GTACAGCGTGGACTGAAGCATATGCGAATGACTCGAAATATCGGTTTAAAGGCGTTATTTAAAGTCGCTAACGTTTCGCA
AAGCGAAATTACAGAAGAAAGCATCGGATTCTCACTCGCACCTCGTATTAATGCGGTTGGTCGTTTGGAAGATGCAACAC
CTGCTGTGCACCTTTTATTATCAGAAGATCCAGAGGAAGCGAAAGAGCTAGCTGAAGAAATTGATGAGCTGAACAAACTT
CGAAAAGATATTGTAAAGCAAATTACTGAAGAAGCTATCGCTGAAGTCGAAAATAATTTCCCGCCAGAAGAAAATAAAGT
ATTAGTCCTTGCAAAAGAAGGTTGGAATCCAGGGGTAATTGGAATTGTCGCTTCTAAGTTAGTTGAACGTTTTTATCGTC
CGACGATTGTATTAAGCATTGATCCTCATAAAGAAACAGCAAAGGGATCGGCTCGTAGTATTGCAGGATTTGATTTGTTT
GCAAACCTTTCAGATTGCCGTGAATTGTTACCGCATTTCGGAGGTCATCCGATGGCAGCGGGAATGACGCTTAATATGAA
TGATGTGGATGAATTAAGACGCAGGTTAAATGAGCAAGCAGATGTAATTTTAACAGCAGAAGATTTTATTCCGATTACAG
CAGTGGATGCATTTTGTAAAGTAGAGGATGTAACGCTTGCCGCGATTGAGGATATGCAAAAGTTAGCGCCATTTGGCGTA
GGAAATCCGAAACCGCGTATTGCGGTGAAAGATGCCGACTTAGAAAGTATCCGTGCAATTGGATCTGATGGTTCGCATTT
AAAAATGGCTCTTCGTGATGGACAAGCAACACTGGACACGATTGGATTTGGATTTGGTACTTATGCGAAAGAAATCTCTC
CAGTTGCGAAGGTTTCTGTAGTAGGAGAAGTGTCCATTAATGAATGGAATAACTTTAAAAAGCCGCAATTAATGGTGCAA
GATATTGCTGTAGAGGCATGGCAATTATTTGACTGGCGTAGTATGCGTAATGTAGAAGCAAATTTAGCAGAACTTCCGAA
AGAAAAAATAACAATGGTGTATTTTTCTGAAGAGGTATTGAATAAATTTTCTTTAGAAGGCTATAAAGAACAGCTCGTGC
ATGCATCAGAGGTAGCTCATTTTGATGATCAATACATTGTGCTACTTGATTTGCCGAAAGGAACAGATGAATTAGGTGAT
GTATTTAAGGCTGGATTCCCAGCTCGAATTTACACGCTATTTTATCAAGAGAATAATCATTTATTTAGTACGGTCCCAAC
GAGGGAACATTTTAAATGGTACTATTCTTTCTTAAGCCAAAAAAAACCATTTTCTTTAAGGCAATATGGGGAACAACTGT
GTCGTCATAAAGGTTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GACGGTGTCATTTTTATGGCAGATAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAGAAAGAATTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTGATCATAAAT
AA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(455-561)

DHHA1 domain (DHHA1)

(315-439)

DHH family (DHH)

(82-226)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.645

100

0.528