Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   MK860_RS12795 Genome accession   NZ_CP092829
Coordinates   2510068..2512392 (-) Length   774 a.a.
NCBI ID   WP_217012248.1    Uniprot ID   -
Organism   Bacillus pumilus strain MS32     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2505068..2517392
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MK860_RS12780 (MK860_12720) dtd 2506717..2507160 (-) 444 WP_044140029.1 D-aminoacyl-tRNA deacylase -
  MK860_RS12785 (MK860_12725) - 2507179..2509389 (-) 2211 WP_044140028.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  MK860_RS12790 (MK860_12730) - 2509532..2510044 (-) 513 WP_003216770.1 adenine phosphoribosyltransferase -
  MK860_RS12795 (MK860_12735) recJ 2510068..2512392 (-) 2325 WP_217012248.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  MK860_RS12800 (MK860_12740) - 2512474..2512827 (-) 354 WP_058014559.1 lipopolysaccharide assembly LapA domain-containing protein -
  MK860_RS12805 (MK860_12745) - 2512930..2513419 (-) 490 Protein_2481 cation:proton antiporter regulatory subunit -
  MK860_RS12810 (MK860_12750) secDF 2513605..2515833 (-) 2229 WP_003216337.1 protein translocase subunit SecDF -
  MK860_RS12815 (MK860_12755) - 2515875..2516171 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 87375.07 Da        Isoelectric Point: 4.9246

>NTDB_id=660605 MK860_RS12795 WP_217012248.1 2510068..2512392(-) (recJ) [Bacillus pumilus strain MS32]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVKRGLEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKELSAKVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGKLPTQLLDLAAIGTIADLVPLHDENRWL
AKKGLMQLRQSNRPGLKALLKEAGATLEEANEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDKDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEETGDDQSAIVVAKAGWNPGVVGIVASKLTDKFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLRADDVPLLRERLNQYANETLTEEDFIPIQRVDAVCKVDELTVEAIEEVGMLSPFGMQN
PKPFIMIEDVRLEEIRTIGANQNHIKMSLKDEDKLLDCVGFHQGQLKEEVVTGSRISVIGEMSINEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKTISTLDPEKYVVVCFDEETKQDVNIPIHHVHAENVSTFDVEGKYVVFADVPADEHLLRQLLE
NQKPARVYTVFQRKEDHFMSAFPSRDQFKWFYGFLLKRGSFPLKEQGMELAKHRGWTKDTMIFMTKVFFELGFVKIENGV
LSIVRDAPKKDLTASASYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=660605 MK860_RS12795 WP_217012248.1 2510068..2512392(-) (recJ) [Bacillus pumilus strain MS32]
ATGTTATTATCCAAAATGCGCTGGGAATATGAACATCCAAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCGCTTCTTGTAAAAAGAGGACTCGAAGAAGTAGAAGAAGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTTCTATTAAAAGGAATGAAAGAAGCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGACTATGATGCGGATGGTGTGACAAGTACGTCTGTGCTTTTACATACATTAAAAGAGTT
GTCTGCGAAAGTAGACTTTTATATTCCAGATCGATTTAAAGAAGGCTATGGGCCTAATGAACAGGCTTTTCGATATATCA
AAGAGCAAGGCGCATCGCTTGTCATCACCGTCGATACAGGCATTGCAGCTGTAAATGAAGCACGTATTGCAAAAGAAATA
GGGTTAGACCTGATCATTACAGATCATCATGAACCAAGTGAGGAGCTTCCTGAGGCACTTGCGATTGTCCATCCTAAGCA
GCCGGGATGTGAGTATCCGTTTAAAGAACTTGCCGGTGTAGGCGTAGCGTTTAAAGTGGCGCATGCTCTTCTTGGAAAGC
TGCCAACTCAGCTGTTAGATCTTGCGGCAATTGGGACAATAGCGGATCTTGTGCCGCTTCATGATGAGAATAGATGGCTC
GCTAAAAAAGGGCTTATGCAGCTTAGGCAGTCGAATAGACCGGGGCTGAAGGCGTTATTAAAAGAAGCAGGTGCTACTCT
TGAAGAAGCAAATGAGGAAACGGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTAGGGCGAATTGAACAGGCCGATC
CAGCTGTTCATCTTCTCATGACAGAGGATAAGGATGAAGCAGAAGAACTCGCTCGTTTTGTACAAGAACTGAATAAAGAA
CGTCAAAAAATTGTGAGTACGATCACAGAAGAAGCCATTCAAATGGTGGAAGAGACAGGTGATGACCAGTCTGCAATCGT
TGTAGCGAAAGCTGGCTGGAATCCGGGTGTTGTCGGAATTGTTGCCTCTAAATTGACGGATAAATTTTCTCGTCCGGCGA
TTGTGCTTGGAATAGATGAAGAGACGCAAATGGCAAAAGGATCAGCGCGGAGTATTCCTGGCTTCGATCTATTTTTCCAT
TTAAGCAAATGCCGCGACATTCTGCCGCATTTTGGCGGGCACCCGATGGCTGCGGGTATGACACTTCGGGCGGATGATGT
TCCTTTACTAAGGGAAAGATTAAATCAATATGCGAATGAAACGTTGACAGAAGAAGATTTTATTCCGATTCAGCGGGTTG
ATGCTGTATGCAAGGTAGACGAATTGACGGTTGAAGCGATTGAAGAAGTGGGGATGCTGTCACCATTTGGCATGCAGAAT
CCAAAGCCGTTCATCATGATTGAAGATGTGAGACTTGAAGAAATCCGAACAATTGGAGCAAATCAGAATCATATTAAAAT
GTCTCTGAAAGATGAAGATAAGCTGCTTGACTGTGTCGGTTTTCATCAAGGTCAATTAAAAGAAGAAGTTGTCACAGGTA
GCCGCATTTCCGTCATTGGTGAAATGTCCATCAATGAATGGAATAACCGGAAGAAACCACAGCTCATGCTAAAAGATGCC
CGAGTAGATGAATGGCAGCTGTTTGATTTGCGCGGCAAAAAGGATTGGGAAAAGACCATCTCCACGCTTGATCCAGAAAA
ATATGTGGTGGTCTGTTTTGACGAAGAAACAAAACAGGATGTCAATATCCCGATTCATCATGTCCATGCAGAAAATGTTT
CAACATTCGATGTAGAAGGCAAGTATGTCGTCTTTGCAGATGTTCCTGCAGATGAACACTTGCTAAGGCAGCTGTTAGAA
AACCAAAAGCCAGCTAGAGTTTATACGGTTTTCCAAAGAAAAGAAGATCATTTCATGTCCGCTTTCCCTAGCCGTGATCA
ATTCAAATGGTTTTATGGATTCTTGTTAAAACGAGGAAGTTTCCCTCTCAAGGAACAAGGAATGGAGCTTGCGAAACATA
GGGGCTGGACAAAAGATACAATGATTTTTATGACAAAGGTGTTTTTTGAACTTGGTTTTGTTAAAATAGAGAATGGTGTG
CTGTCAATAGTACGTGATGCCCCTAAAAAAGATTTAACAGCATCAGCATCCTATACAGCAAAACAACGATTGATGGAACT
CGATCAAACACTAACATATTCCTCAGCCAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCTCACCTGTGC
TTTGA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(453-559)

DHHA1 domain (DHHA1)

(309-437)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-763)

DHH family (DHH)

(83-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.181

100

0.674