Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   MK860_RS05250 Genome accession   NZ_CP092829
Coordinates   1085901..1089410 (+) Length   1169 a.a.
NCBI ID   WP_065098901.1    Uniprot ID   -
Organism   Bacillus pumilus strain MS32     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1080901..1094410
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MK860_RS05220 (MK860_05210) lepB 1081411..1081923 (+) 513 WP_041815362.1 signal peptidase I -
  MK860_RS05225 (MK860_05215) - 1081960..1083105 (-) 1146 WP_278179500.1 AbrB family transcriptional regulator -
  MK860_RS05230 (MK860_05220) - 1083372..1083794 (+) 423 WP_217012826.1 BlaI/MecI/CopY family transcriptional regulator -
  MK860_RS05235 (MK860_05225) - 1083787..1084626 (+) 840 WP_217012827.1 M56 family metallopeptidase -
  MK860_RS05240 (MK860_05230) - 1084683..1085093 (+) 411 WP_217012828.1 DoxX family protein -
  MK860_RS05245 (MK860_05235) - 1085290..1085736 (-) 447 WP_044139865.1 ferritin-like domain-containing protein -
  MK860_RS05250 (MK860_05240) addB 1085901..1089410 (+) 3510 WP_065098901.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  MK860_RS05255 (MK860_05245) addA 1089388..1093086 (+) 3699 WP_065098900.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  MK860_RS05260 (MK860_05250) sbcD 1093113..1094288 (+) 1176 WP_106064954.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134618.39 Da        Isoelectric Point: 5.2648

>NTDB_id=660578 MK860_RS05250 WP_065098901.1 1085901..1089410(+) (addB) [Bacillus pumilus strain MS32]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEQKDKFKVFKKASDKPGFVEQIEKTMAEFKRYCMLPEDIEKISASSMHSEYTEERRAAE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLAQQIPSAEEIKGAHIYIDGFYQFTPQQLLVIEQLLLHAAKITAAFTVDRS
YHDRQPNELDLFRMTGKTYFQLYQLAKECGADISENIFERNHRHLYTPDLAYLEHQYEQRPVQPYQENTPHLTVSKSANK
RAEIEGVARDILDLVREKGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPAEITKNKAREHADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IAPPLYQLQKGLKKAQKVRDMVEAVYVFLEEIQVPDKLEKARLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVDMLGDEEL
SFPLFQQMIDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCTFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSRHLYLSYAASDAEGRSLLPSTLIKYCQELMPNHQQALYVLDPEQLEDEEQLKFVANEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNYLMTSPHRDVSKNIMSSLFFTNRAQPLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKEDCVNYSRYAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKLSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGVEATPAGILYFHIHDPLIQAPIPLAEDEIEQEIFKKFKMKGL
LLEDVEAVKLMDQTLDSGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTQHVRRTFEEAGERITNGEVAINPYKLKDQTP
CRFCSFKSICQFDESIEDNDFRVLTSEKDDVVIERIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=660578 MK860_RS05250 WP_065098901.1 1085901..1089410(+) (addB) [Bacillus pumilus strain MS32]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCTTGATCCGTTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTTTTAATGGAATATGAGCTTGCGAAAA
CGTCTGAAGCTGGTGGAATGATCAGAGCTAAAGTATTTAGTTTCACTCGTCTTGCTTGGTCTATTTTACAGCAGACTGGT
GGAGCGAACCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTGATTGAAGAGCAGAAAGACAAGTT
TAAAGTATTCAAAAAAGCGAGTGATAAGCCGGGGTTTGTGGAGCAAATCGAAAAAACGATGGCTGAATTCAAAAGATACT
GTATGCTGCCGGAGGATATTGAGAAAATTTCAGCGTCTAGCATGCATTCAGAGTATACAGAAGAAAGACGGGCAGCCGAA
AAATTGCATGATCTCCATGTTCTTTATCAGCAGATGGAGCAGCATTTACAAGATGAATATGTGCACTCAGAAGACTATTT
GACTCTTCTTGCCCAGCAAATTCCTTCAGCTGAAGAAATAAAAGGAGCACACATCTATATAGATGGTTTTTATCAATTTA
CACCGCAGCAGCTTCTTGTCATTGAACAGCTCTTGCTGCATGCAGCAAAAATAACTGCGGCTTTCACAGTAGATCGATCC
TATCATGATAGGCAGCCAAATGAACTTGATTTATTTCGTATGACAGGTAAAACGTATTTCCAGCTCTATCAGCTTGCTAA
AGAGTGCGGAGCTGACATTTCTGAAAACATTTTTGAAAGAAATCATCGGCACCTTTATACACCAGATCTTGCTTATTTAG
AACACCAATATGAGCAGCGGCCCGTGCAGCCATATCAGGAAAACACCCCTCATCTCACAGTGTCTAAAAGTGCAAATAAA
CGAGCTGAAATTGAGGGAGTAGCAAGAGATATTCTTGATTTAGTGAGAGAAAAAGGACTCAGACTGCGAGATATCTCGAT
TGTAGCGCGGCATGTAGATGACTATAAAGATACGTTAAAAGAGGTTTTTAGAGATTACGATATTCCTTTTTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATCGAATTGATTCGTTCGAGCTTGGATGTCATAAAAGGAAATTGGCGGTAT
GAAGCGGTATTTCGCTGCGTGAAAACAGAGTTCTTATTTCCGGCTGAAATCACTAAGAACAAAGCGAGAGAGCATGCAGA
TCAGCTTGAAAACTATTGTATTGCTTATGGTGTAAAAGGAGAGCGCTGGACAAACGGTTCCCGGTTTCATTACAGGCGTT
TCCAATCATTAGATGAGGATTTCGGACAAACAGATCAAGAAATTGAAATGGAACAAATGCTAAATGACGTCAAAGAATGG
ATCGCGCCCCCGCTTTACCAGTTGCAAAAAGGGCTCAAAAAAGCGCAAAAGGTAAGGGACATGGTAGAGGCTGTCTATGT
CTTTTTAGAAGAGATACAAGTACCAGATAAGCTTGAAAAAGCGAGGCTTGAGGCTGAAGAAGCAGGTCGATTAGCTGAAG
CAATGCAGCACGGGCAAGTATGGGATGCGGTGATTCAATTAATGGATGAATTTGTTGACATGCTAGGTGACGAAGAGCTC
TCATTTCCTTTATTCCAACAGATGATAGATACAGGATTAGCTTCTCTAAAATTCGCTTTGATTCCGCCTTCACTCGACCA
AGTATTTATCGGAAGTATGGATTTATCCAGAATGTATCAAGTGAAGTGTACGTTTATCATTGGTGTAAATGATGGCGTTA
TTCCCGCACGCCCCTCTGATGAGAGTGTATTGTCTGAGGATGACCGAGAATGGTTAAAACGAGCAGGAGCAGAGCTAGCA
GAGACAGGAAAGGAACGGCTGCTAGATGAGCAATTTTTAATTTACCAAGCGTTATCAAGTCCATCCCGACATTTGTATCT
ATCCTATGCGGCTTCTGATGCAGAAGGACGTTCTCTATTGCCTTCGACACTCATCAAGTATTGCCAAGAGCTTATGCCAA
ATCATCAGCAGGCTCTTTATGTGTTAGATCCAGAACAGCTAGAAGATGAGGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTTTCGTTGTCGTATACCGTCTCACAGCTGCAGCAATGGCTCAATCAATATCCCATTAGCGGTGTATGGTGGAGTGTCTA
TAACTACTTAATGACATCTCCTCATCGGGATGTATCAAAAAACATCATGTCAAGTTTGTTTTTTACAAATCGAGCGCAGC
CATTAAAACCTAATGTCACAAAAGAACTTTACGGTGACCATATTCAGGGCAGTGTTTCAAGAATGGAGAAATTCAATGCG
TGTGCATTTTCTCACTTTGCTTCCCACGGTTTAAAACTAAAGGATCGACAATTTTACAAATTGGAAGCACCCGATATTGG
CCAGTTGTTTCACTCGGCTTTGAAGCATATTTCAGATACGCTAGTTGAACAAAAAAAGGATTGGAAAAACCTAACGAAGG
AAGATTGTGTAAACTACTCAAGATATGCAATCGAACAGCTTGCTCCGCGTCTTCAAAAGGAGATTCTATTAAGCTCTAAT
CGGCATGCTTATATTAAAGAAAAGCTTCAGAAAATCTTAATTCGAGTCTCTTCTATATTAAGTGAACATGCCAAATTGAG
TGGATTTTCTCCAGTAGGGCTTGAGTTAGGTTTTGGGGGCCAAGGGCCTTTGCCGCCGTTTACTTTTCAATTAAAGAATG
GCTGCACGATGGAGCTTGTCGGAAGAATTGACAGGGTAGATAAAGCAGAAGGTTCAAAAGGGCTGCTTTTAAGAATCGTC
GATTACAAATCCAGTGAGAAAGGTCTTGACCTAGCAGAAGTATACTATGGTCTTGCCTTGCAAATGCTCACATATTTAGA
CCTAACCATTACGTATTCGAAAGAGTGGCTCGGCGTAGAAGCAACGCCTGCTGGCATTTTATATTTCCATATTCATGATC
CGCTCATTCAAGCACCGATTCCACTTGCAGAGGATGAAATTGAGCAGGAAATATTCAAGAAATTTAAAATGAAGGGTTTA
TTGCTTGAAGATGTGGAAGCAGTAAAGCTAATGGATCAAACGCTTGATTCAGGTAGATCGCAAGTCATTCAAGCCGGTTT
GAAAAAGGATGGGTCTTTCCGTTCTGATTCGGCAGTTTTAAGTGAAGATCATTTCCATATACTCACACAACACGTTCGGC
GCACATTTGAAGAAGCAGGCGAAAGAATTACAAATGGAGAGGTCGCAATTAATCCATATAAATTAAAGGATCAAACACCT
TGCCGCTTTTGTTCATTCAAGTCTATTTGCCAATTCGATGAATCAATAGAAGATAATGATTTTAGGGTGCTCACCTCTGA
AAAGGATGATGTTGTGATAGAACGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

66.236

99.316

0.658