Detailed information NTG0660215

Bioinformatically predicted

Overview

• Name: recD2
• Type: Machinery gene
• Locus tag: MK616_RS13105
• Genome accession: NZ_CP092778
• Coordinates: 2684126..2686504 (-)
• Length: 792 a.a.
• NCBI ID: WP_014418522.1
• Uniprot ID: I2C8C6
• Organism: Bacillus amyloliquefaciens strain WF2020
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2679126..2691504

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MK616_RS13070 (MK616_13070)	-	2680198..2680926 (+)	729	WP_014418519.1	amino acid ABC transporter ATP-binding protein	-
MK616_RS13075 (MK616_13075)	-	2680947..2681774 (+)	828	WP_007408208.1	transporter substrate-binding domain-containing protein	-
MK616_RS13080 (MK616_13080)	-	2681831..2682481 (+)	651	WP_100261915.1	amino acid ABC transporter permease	-
MK616_RS13085 (MK616_13085)	-	2682500..2683156 (+)	657	WP_100261916.1	amino acid ABC transporter permease	-
MK616_RS13090 (MK616_13090)	-	2683192..2683323 (-)	132	WP_003152741.1	YrzQ family protein	-
MK616_RS13095 (MK616_13095)	-	2683345..2683536 (-)	192	WP_003152738.1	hypothetical protein	-
MK616_RS13100 (MK616_13100)	-	2683549..2684007 (-)	459	WP_021494376.1	PRC-barrel domain-containing protein	-
MK616_RS13105 (MK616_13105)	recD2	2684126..2686504 (-)	2379	WP_014418522.1	ATP-dependent RecD-like DNA helicase	Machinery gene
MK616_RS13110 (MK616_13110)	-	2686523..2687143 (-)	621	WP_088461189.1	tetratricopeptide repeat protein	-
MK616_RS13115 (MK616_13115)	mnmA	2687231..2688346 (-)	1116	WP_012118077.1	tRNA 2-thiouridine(34) synthase MnmA	-
MK616_RS13120 (MK616_13120)	-	2688383..2689522 (-)	1140	WP_021494378.1	cysteine desulfurase family protein	-
MK616_RS13125 (MK616_13125)	cymR	2689541..2689957 (-)	417	WP_003152730.1	cysteine metabolism transcriptional regulator CymR	-
MK616_RS13130 (MK616_13130)	-	2690152..2691420 (+)	1269	WP_003152729.1	replication-associated recombination protein A	-

Sequence

Protein

• Length: 792 a.a.
• Molecular weight: 88412.10 Da
• Isoelectric Point: 4.9490

>NTDB_id=660215 MK616_RS13105 WP_014418522.1 2684126..2686504(-) (recD2) [Bacillus amyloliquefaciens strain WF2020]
MDQLNIEEQPYVKGVIHTVIYHNDTNLYTVLKVKVAETSEAIEDKVVSVTGYFPLLQEDETYTFYGKTATHPKFGLQFQA
EHFKKEIPTTKEGIIHYLSSDLFEGIGKKTAEEIVKKLGDGALNKILTDASVLYDVPKMSKKKADKLAGELQRHQGLEQI
MISLNQYGFGPQLSMKIYQAYETETLEKIQENPYQLVKDVEGIGFVKADELGGKMGLSGNHPERIKAAILYTIESTCLSE
GHTYIETKQLIINTQTLLNQSAEGGRVTEMDAADGIIALGESKDIVIEGERCYFPSLFYAEQNVAKRVLDIAAQTEYADQ
FPESEFLLALGELEERMNVQYAPSQKDAIQKALSSPMLLLTGGPGTGKTTVIKGIVELYSELHGVSLDPGDYKKDETFPV
LLAAPTGRAAKRMSESTGLPAVTIHRLLGWNGAEGFTYNEDQPIEGKLIIIDEASMLDIWLASHLFKALPKHIQLIVVGD
EDQLPSVGPGQVLRDLLASEVLPTVRLTDIYRQAEGSSIVELAHDMKKGLLPKGLTAPTKDRSFIRCNSSQMKEVVDQVV
SNALKKGYTSRDIQVLAPMYRGKAGINELNMLLQDIMNPPKEKRRELKFGDVVYRTGDKILQLVNQPENNVFNGDIGEIV
SIFYAKENTEKEDMVVVSFDGNEITFTKKDFSQFTHAYCCSIHKSQGSEFPIVVLPVVKGYYRMLRRNLLYTAITRAKKF
LILCGEEEALEWGVKNNDATVRQTSLKNRLTEQTEEMDAELAALQKELPFSVYDANIGMEGITPFDFMEEKA

Nucleotide

• Length: 2379 bp

>NTDB_id=660215 MK616_RS13105 WP_014418522.1 2684126..2686504(-) (recD2) [Bacillus amyloliquefaciens strain WF2020]
GTGGACCAGCTGAACATCGAAGAACAGCCGTATGTAAAAGGCGTCATTCATACGGTCATTTATCACAATGACACCAACCT
GTATACAGTGCTGAAAGTCAAGGTTGCAGAAACGTCTGAGGCGATCGAAGACAAGGTCGTATCCGTAACGGGCTACTTCC
CTCTGCTGCAAGAAGATGAGACCTACACATTTTACGGAAAAACCGCCACGCATCCTAAGTTCGGCCTGCAATTTCAGGCT
GAACACTTTAAAAAAGAAATTCCGACCACAAAAGAAGGGATCATTCACTATTTATCAAGTGATCTGTTTGAAGGAATCGG
AAAAAAAACGGCGGAAGAAATCGTCAAAAAGCTCGGAGACGGCGCGCTCAATAAAATTCTGACGGATGCGTCGGTGCTTT
ACGATGTGCCGAAAATGTCGAAAAAGAAAGCGGATAAGCTTGCCGGAGAGCTGCAGCGGCATCAGGGGCTTGAGCAGATT
ATGATCTCTCTGAACCAATACGGTTTCGGGCCCCAGCTATCAATGAAAATATATCAGGCCTACGAAACGGAAACCCTGGA
GAAAATTCAGGAAAATCCTTATCAGCTTGTAAAAGACGTTGAAGGCATCGGCTTTGTGAAAGCTGACGAGCTGGGCGGAA
AAATGGGTCTGTCAGGAAACCACCCGGAGCGGATCAAAGCGGCAATCTTATATACGATTGAATCGACCTGTCTTTCAGAA
GGCCATACGTACATAGAGACAAAGCAGCTGATTATCAATACGCAAACGCTCCTGAATCAGTCAGCTGAAGGCGGCCGCGT
TACGGAGATGGATGCGGCTGACGGCATCATCGCCCTCGGGGAGAGTAAGGATATCGTCATAGAAGGAGAGCGATGCTATT
TTCCGTCCTTGTTTTACGCCGAACAAAATGTCGCAAAACGCGTGCTCGACATTGCGGCTCAGACGGAGTATGCCGATCAA
TTTCCTGAATCGGAATTTCTGCTTGCACTCGGTGAACTGGAAGAACGGATGAATGTTCAATACGCACCAAGCCAAAAGGA
TGCCATTCAAAAAGCGCTGTCTTCACCGATGCTTTTACTGACCGGAGGACCCGGGACGGGGAAAACGACCGTTATTAAAG
GAATCGTAGAATTATACAGTGAACTGCACGGAGTATCGCTTGACCCCGGTGATTATAAAAAAGACGAGACGTTCCCGGTG
CTGCTTGCCGCACCGACGGGAAGAGCCGCAAAACGGATGAGCGAATCTACGGGGCTGCCGGCTGTTACGATTCACAGGCT
GCTCGGCTGGAATGGCGCTGAAGGCTTTACCTACAATGAAGATCAGCCGATTGAAGGGAAACTGATCATTATAGACGAAG
CGAGTATGCTCGATATCTGGCTCGCCAGCCATTTGTTTAAGGCGCTTCCTAAGCACATTCAGCTCATTGTCGTCGGTGAT
GAAGACCAGCTTCCTTCAGTAGGACCGGGGCAGGTGCTCAGAGACCTTTTGGCTTCTGAGGTGCTGCCGACCGTGCGTCT
GACAGATATTTACCGTCAGGCTGAAGGATCATCCATTGTGGAACTTGCGCATGATATGAAAAAAGGACTGCTTCCAAAGG
GGCTGACCGCGCCGACCAAGGACCGCTCATTTATCCGCTGTAATTCTTCACAGATGAAAGAAGTCGTGGATCAGGTCGTC
TCGAACGCGTTAAAAAAGGGGTATACCTCCAGGGATATTCAAGTGCTCGCCCCGATGTACAGGGGAAAAGCGGGAATTAA
CGAGCTGAATATGCTTCTTCAAGACATCATGAATCCGCCGAAAGAAAAAAGGCGGGAGCTGAAATTCGGTGATGTCGTCT
ACAGGACGGGCGATAAAATATTACAGCTTGTCAACCAGCCGGAAAACAATGTGTTTAACGGCGATATCGGCGAAATCGTG
TCCATTTTTTATGCCAAAGAAAATACGGAAAAAGAAGACATGGTCGTCGTCTCGTTTGACGGCAACGAAATCACCTTTAC
GAAAAAGGATTTCAGCCAGTTTACCCACGCTTACTGCTGTTCCATTCATAAGTCGCAGGGAAGCGAATTCCCGATTGTCG
TCCTGCCTGTCGTCAAAGGCTACTATCGAATGCTCAGGAGAAATCTGCTTTATACCGCCATAACAAGAGCGAAAAAATTT
CTGATTCTTTGCGGAGAAGAAGAAGCGCTTGAATGGGGCGTCAAAAACAATGATGCCACCGTCAGACAGACGTCGCTTAA
AAACAGGCTCACAGAGCAGACTGAAGAGATGGATGCAGAACTGGCCGCCCTGCAAAAAGAGCTGCCGTTCAGTGTATATG
ATGCGAACATCGGAATGGAAGGCATCACGCCGTTTGACTTTATGGAAGAAAAAGCGTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix containing domain (HHH_RecD2) (154-244)

• AAA domain (AAA_30) (341-548)

• ATP-dependent RecD-like DNA helicase SH3 domain (SH3_13) (589-659)

• UvrD-like helicase C-terminal domain (UvrD_C_2) (676-724)

3D structure

• AlphaFold DB: I2C8C6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recD2	Bacillus subtilis subsp. subtilis str. 168	88.622	99.874	0.885