NTDB

Overview

Name	comGB	Type	Machinery gene
Locus tag	MKI65_RS07490	Genome accession	NZ_CP092565
Coordinates	1568749..1569819 (-)	Length	356 a.a.
NCBI ID	WP_000776424.1	Uniprot ID	-
Organism	Staphylococcus aureus strain MRSA-WC061
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1563749..1574819

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MKI65_RS07450 (MKI65_001475)	gcvPA	1563926..1565272 (-)	1347	WP_000019691.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
MKI65_RS07455 (MKI65_001476)	gcvT	1565292..1566383 (-)	1092	WP_000093349.1	glycine cleavage system aminomethyltransferase GcvT	-
MKI65_RS07460 (MKI65_001477)	-	1566542..1567066 (-)	525	WP_001015120.1	shikimate kinase	-
MKI65_RS07465 (MKI65_001478)	-	1567056..1567202 (-)	147	WP_001792109.1	hypothetical protein	-
MKI65_RS07470 (MKI65_001479)	comGF	1567299..1567796 (-)	498	WP_001788897.1	competence type IV pilus minor pilin ComGF	Machinery gene
MKI65_RS07475 (MKI65_001480)	comGE	1567714..1568013 (-)	300	WP_000844410.1	hypothetical protein	Machinery gene
MKI65_RS07480 (MKI65_001481)	comGD	1568000..1568446 (-)	447	WP_001788899.1	competence type IV pilus minor pilin ComGD	Machinery gene
MKI65_RS07485 (MKI65_001482)	comGC	1568424..1568735 (-)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
MKI65_RS07490 (MKI65_001483)	comGB	1568749..1569819 (-)	1071	WP_000776424.1	competence type IV pilus assembly protein ComGB	Machinery gene
MKI65_RS07495 (MKI65_001484)	comGA	1569791..1570765 (-)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
MKI65_RS07500 (MKI65_001485)	-	1570817..1571440 (-)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
MKI65_RS07505 (MKI65_001486)	-	1571437..1571766 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
MKI65_RS07510 (MKI65_001487)	-	1571766..1572752 (-)	987	WP_000161311.1	ROK family glucokinase	-
MKI65_RS07515 (MKI65_001488)	-	1572749..1572952 (-)	204	WP_000087561.1	YqgQ family protein	-
MKI65_RS07520 (MKI65_001489)	-	1572933..1574396 (-)	1464	WP_001017857.1	rhomboid family intramembrane serine protease	-

Sequence

Protein

Download Length: 356 a.a. Molecular weight: 41433.82 Da Isoelectric Point: 10.0606

                                >NTDB_id=658950 MKI65_RS07490 WP_000776424.1 1568749..1569819(-) (comGB) [Staphylococcus aureus strain MRSA-WC061]

MKLQWINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG

YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNVQLS

SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ

SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK

QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide

Download Length: 1071 bp

                                >NTDB_id=658950 MKI65_RS07490 WP_000776424.1 1568749..1569819(-) (comGB) [Staphylococcus aureus strain MRSA-WC061]

GTGAAACTACAATGGATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGCCCAACAAATCGATTTACT

TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA

AAAATAAGCAATTAGGTACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAATCAAATATTATCACTGATAGGT

TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATTATAGACGTTCTAGAAGAAACCGTAAA

TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTTTCTATCTTTA

TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATGTTCAACTATCT

TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT

GGCTATTATTATGAAATTAATTTATAACAATTTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT

CAGGCTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA

TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA

AATGGGATATGGTTTGCCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG

AAAAGAGAGGGAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA

CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC

AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGB	Staphylococcus aureus MW2	99.719	100	0.997
comGB	Staphylococcus aureus N315	99.719	100	0.997

Detailed information NTG0658950