Detailed information NTG0657590

Bioinformatically predicted

Overview

• Name: recD2
• Type: Machinery gene
• Locus tag: MJE83_RS14395
• Genome accession: NZ_CP092446
• Coordinates: 2856365..2858743 (-)
• Length: 792 a.a.
• NCBI ID: WP_007408204.1
• Uniprot ID: A7Z743
• Organism: Bacillus velezensis strain R-71003
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2851365..2863743

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MJE83_RS14360 (MJE83_14360)	-	2852437..2853165 (+)	729	WP_007408209.1	amino acid ABC transporter ATP-binding protein	-
MJE83_RS14365 (MJE83_14365)	-	2853186..2854013 (+)	828	WP_007408208.1	transporter substrate-binding domain-containing protein	-
MJE83_RS14370 (MJE83_14370)	-	2854070..2854720 (+)	651	WP_032866635.1	amino acid ABC transporter permease	-
MJE83_RS14375 (MJE83_14375)	-	2854739..2855395 (+)	657	WP_069006948.1	amino acid ABC transporter permease	-
MJE83_RS14380 (MJE83_14380)	-	2855431..2855562 (-)	132	WP_003152741.1	YrzQ family protein	-
MJE83_RS14385 (MJE83_14385)	-	2855584..2855775 (-)	192	WP_003152738.1	hypothetical protein	-
MJE83_RS14390 (MJE83_14390)	-	2855788..2856246 (-)	459	WP_007408205.1	PRC-barrel domain-containing protein	-
MJE83_RS14395 (MJE83_14395)	recD2	2856365..2858743 (-)	2379	WP_007408204.1	ATP-dependent RecD-like DNA helicase	Machinery gene
MJE83_RS14400 (MJE83_14400)	-	2858762..2859412 (-)	651	WP_015240287.1	tetratricopeptide repeat protein	-
MJE83_RS14405 (MJE83_14405)	mnmA	2859470..2860585 (-)	1116	WP_012118077.1	tRNA 2-thiouridine(34) synthase MnmA	-
MJE83_RS14410 (MJE83_14410)	-	2860622..2861761 (-)	1140	WP_007408201.1	cysteine desulfurase family protein	-
MJE83_RS14415 (MJE83_14415)	cymR	2861780..2862196 (-)	417	WP_003152730.1	cysteine metabolism transcriptional regulator CymR	-
MJE83_RS14420 (MJE83_14420)	-	2862391..2863659 (+)	1269	WP_015240288.1	replication-associated recombination protein A	-

Sequence

Protein

• Length: 792 a.a.
• Molecular weight: 88364.04 Da
• Isoelectric Point: 4.9490

>NTDB_id=657590 MJE83_RS14395 WP_007408204.1 2856365..2858743(-) (recD2) [Bacillus velezensis strain R-71003]
MDQLNIEEQPYVKGVIHTVIYHNDTNLYTVLKVKVAETSEAIEDKVVSVTGYFPLLQEDETYTFYGKTATHPKFGLQFQA
EHFKKEIPTTKEGIIHYLSSDLFEGIGKKTAEEIVKKLGDGALNKILTDASVLYDVPKMSKKKADKLAGELQRHQGLEQI
MISLNQYGFGPQLSMKIYQAYETETLEKIQENPYQLVKDVEGIGFVKADELGGKMGLSGNHPERIKAAILYTIESTCLSE
GHTYIETKQLIINTQTLLNQSAEGGRVTEMDAADGIIALGESKDIVIEGERCYFPSLFYAEQNVAKRVLDIAAQTEYADQ
FPESEFLLALGELEERMNVQYAPSQKDAIQKALSSPMLLLTGGPGTGKTTVIKGIVELYSELHGVSLDPGDYKKDEAFPV
LLAAPTGRAAKRMSESTGLPAVTIHRLLGWNGAEGFTYNEDQPIEGKLIIIDEASMLDIWLASHLFKALPKHIQLIVVGD
EDQLPSVGPGQVLRDLLASEVLPTVRLTDIYRQAEGSSIVELAHDMKKGLLPKGLTAPTKDRSFIRCNSSQIKEVVDQVV
SNALKKGYTSRDIQVLAPMYRGKAGINELNMLLQDIMNPPKEKRRELKFGDVVYRTGDKILQLVNQPENNVFNGDIGEIV
SIFYAKENTEKEDMVVVSFDGNEITFTKKDFSQFTHAYCCSIHKSQGSEFPIVVLPVVKGYYRMLRRNLLYTAITRAKKF
LILCGEEEALEWGVKNNDATVRQTSLKNRLTEQTEEMDAELAALQKELPFSVYDANIGMEGITPFDFMEEKA

Nucleotide

• Length: 2379 bp

>NTDB_id=657590 MJE83_RS14395 WP_007408204.1 2856365..2858743(-) (recD2) [Bacillus velezensis strain R-71003]
GTGGATCAGCTGAACATCGAAGAACAGCCGTATGTAAAAGGCGTCATTCATACGGTCATTTATCACAATGACACCAACCT
GTATACAGTGCTGAAAGTCAAGGTTGCAGAAACGTCTGAGGCGATCGAAGACAAGGTCGTATCCGTAACGGGCTACTTCC
CTCTGCTGCAAGAAGATGAGACCTACACATTTTACGGAAAAACCGCCACGCATCCTAAGTTCGGCCTGCAATTTCAGGCT
GAACACTTTAAAAAAGAAATTCCGACCACAAAAGAAGGGATCATTCACTACTTATCAAGTGATCTGTTTGAAGGAATCGG
AAAAAAAACGGCGGAAGAAATCGTCAAAAAGCTCGGAGACGGCGCGCTCAATAAAATTCTGACGGATGCGTCGGTGCTTT
ACGATGTGCCGAAAATGTCGAAAAAGAAAGCGGATAAGCTTGCCGGAGAGCTGCAGCGGCATCAGGGGCTTGAGCAGATT
ATGATCTCTCTGAACCAATACGGTTTCGGGCCCCAGCTATCCATGAAAATATATCAGGCCTACGAAACGGAAACACTGGA
GAAAATTCAGGAAAATCCTTATCAGCTTGTAAAAGACGTTGAAGGCATCGGCTTTGTGAAAGCTGACGAGCTGGGCGGAA
AAATGGGTCTGTCAGGAAACCACCCGGAGCGGATCAAAGCGGCTATCTTATATACAATTGAATCGACCTGTCTTTCAGAG
GGCCATACGTACATAGAAACAAAGCAGCTGATAATCAATACGCAAACGCTCCTGAATCAGTCAGCTGAAGGCGGCCGCGT
CACGGAAATGGATGCGGCTGACGGCATCATCGCCCTCGGGGAGAGTAAGGATATCGTCATAGAAGGAGAACGCTGCTATT
TTCCGTCCTTGTTTTACGCCGAACAAAATGTCGCAAAACGCGTGCTCGACATTGCGGCTCAGACGGAGTATGCCGATCAA
TTTCCTGAATCGGAATTTCTGCTTGCACTCGGTGAACTGGAAGAACGGATGAATGTTCAATACGCACCAAGCCAAAAGGA
TGCCATTCAAAAAGCGCTGTCTTCACCGATGCTTTTACTGACCGGAGGACCTGGGACGGGGAAAACGACCGTTATTAAAG
GAATCGTGGAATTATACAGTGAACTGCACGGAGTATCGCTTGACCCCGGTGATTATAAAAAAGACGAGGCGTTCCCGGTG
CTGCTTGCCGCGCCGACGGGAAGAGCCGCAAAACGGATGAGCGAATCTACGGGTCTGCCGGCTGTTACGATACACAGGCT
GCTCGGCTGGAATGGCGCTGAAGGCTTTACCTACAATGAAGATCAGCCGATAGAAGGGAAACTGATCATTATAGACGAAG
CGAGTATGCTCGATATCTGGCTCGCCAGCCATTTGTTTAAGGCGCTTCCTAAGCACATTCAGCTCATTGTCGTCGGTGAT
GAAGACCAGCTTCCTTCAGTAGGACCGGGGCAGGTGCTCAGAGACCTTTTGGCTTCTGAGGTGCTGCCGACCGTGCGCCT
GACAGATATTTACCGTCAGGCTGAAGGATCATCCATTGTGGAACTTGCGCATGATATGAAAAAAGGACTGCTTCCGAAGG
GGCTGACCGCGCCGACTAAGGACCGCTCATTTATCCGCTGTAATTCTTCACAGATTAAAGAAGTCGTGGATCAGGTCGTC
TCGAACGCGTTAAAAAAGGGGTATACCTCCAGGGATATTCAAGTGCTTGCCCCGATGTACAGGGGAAAAGCGGGAATTAA
CGAGCTGAATATGCTTCTTCAAGACATCATGAATCCGCCGAAAGAAAAAAGGCGGGAGCTGAAATTCGGTGATGTCGTCT
ACAGGACGGGCGATAAAATATTACAGCTTGTCAACCAGCCGGAAAACAATGTGTTTAACGGCGATATTGGCGAAATCGTA
TCCATTTTTTATGCCAAAGAAAATACGGAAAAAGAAGACATGGTCGTCGTCTCATTTGACGGCAACGAAATCACCTTTAC
GAAAAAGGATTTCAGCCAGTTTACCCACGCGTACTGCTGTTCCATTCATAAGTCGCAGGGGAGCGAATTCCCGATTGTCG
TCCTGCCTGTCGTCAAAGGCTACTATCGAATGCTCAGGAGAAATCTGCTTTATACCGCCATAACAAGGGCGAAAAAATTT
CTGATTCTTTGCGGAGAAGAAGAAGCGCTTGAATGGGGCGTCAAAAACAATGATGCCACCGTCAGACAGACGTCGCTTAA
AAACAGGCTCACAGAGCAGACTGAAGAGATGGATGCAGAACTGGCCGCCCTGCAAAAAGAGCTGCCGTTCAGTGTATATG
ATGCGAACATCGGAATGGAAGGCATCACGCCGTTTGACTTTATGGAAGAAAAAGCGTAA

Domains

Predicted by InterproScan.

• ATP-dependent RecD-like DNA helicase SH3 domain (SH3_13) (589-659)

• AAA domain (AAA_30) (341-548)

• UvrD-like helicase C-terminal domain (UvrD_C_2) (676-724)

• Helix-hairpin-helix containing domain (HHH_RecD2) (154-244)

3D structure

• AlphaFold DB: A7Z743

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recD2	Bacillus subtilis subsp. subtilis str. 168	88.875	99.874	0.888