Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   MG974_RS05390 Genome accession   NZ_CP092439
Coordinates   1036017..1039517 (+) Length   1166 a.a.
NCBI ID   WP_044053080.1    Uniprot ID   -
Organism   Bacillus velezensis strain 504     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1031017..1044517
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MG974_RS05365 (MG974_05365) - 1031188..1032345 (-) 1158 WP_014304750.1 AbrB family transcriptional regulator -
  MG974_RS05370 (MG974_05370) - 1032513..1033709 (-) 1197 WP_003155154.1 MFS transporter -
  MG974_RS05375 (MG974_05375) - 1033725..1034345 (-) 621 WP_014304751.1 TetR/AcrR family transcriptional regulator -
  MG974_RS05380 (MG974_05380) - 1034584..1035093 (-) 510 WP_003155151.1 ferritin-like domain-containing protein -
  MG974_RS05385 (MG974_05385) - 1035162..1035875 (-) 714 WP_021734194.1 DUF421 domain-containing protein -
  MG974_RS05390 (MG974_05390) addB 1036017..1039517 (+) 3501 WP_044053080.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  MG974_RS05395 (MG974_05395) addA 1039504..1043208 (+) 3705 WP_044053580.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  MG974_RS05400 (MG974_05400) sbcD 1043273..1044445 (+) 1173 WP_044053081.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 133327.91 Da        Isoelectric Point: 5.4981

>NTDB_id=657418 MG974_RS05390 WP_044053080.1 1036017..1039517(+) (addB) [Bacillus velezensis strain 504]
MGVEFLVGRSGSGKTRLIIDSIQDELRREPFGKPIIFLVPDQMTFLMEYELAKTPDIGGMIRAQVFSFSRLAWRVLQHTG
GMNRPFVTTTGVQMLLRKLIEEHKHDFKVYQKASDKAGFTEQVERMLTEFKRYCIEPEDVRRMAESGTASEYRGERMLSE
KLHDLGILYQQMERSLAGHYLHSEDYLTLLAQQLPLADVVKGARVYVDGFYQFTPQELRVLEQLIMHAEHVTFSLTADSP
SAEQAPDELDLFRMTGSTYYKLYQTAKELNADISCKELHGTKRHRHVPELACIESQYDVRPAAAYTGGKEAFTVMQAQNR
RAELEGIAREIQSLVRDGGYRYKDMAILIRQPEDYKDLLKEVFADYGLPYFIDGKASMQHHPLIEFIRSSLDVVKGNWRY
EAVFRCAKTELLFPLDQPEQKIREQVDQLENYCIAYGIKGERWTNGERFVYRRFVSLDEDFAQTDQEIEMEHMLNETREW
IAAPLIRLQNRMKKAKTVQSMAEALYLFLEDTDVPLKLDRKRQRAEEAGNMIEAQQHGQAWDAVIQLLEEFAGMMGEDEI
SLALFQQMLETGTESLHFSLIPPALDQVFVGNMDLSRIYGTSCTFVIGANDGVLPARPDENGVLSDDDREWLKAVGVELS
SAGRERLLDEHFLIYMALSSPSDRLYVSYPIADAEGKTLLPSIVVNRLGELFPDHQEKLSAADPEQVSEEEQLQYLVNKQ
VAQTYTASQLRLWTREYEISDVWWSAYNVLMKESDHQRAKKLFSSLFFRNEAKRLERPVSRQLYGEHIKGSVSRMEAFNA
CQFSHFASHGLQLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLEWRDLTKDQCRHFSYEAVERLAPKLQKEILLSSN
RHFYVKEKLQKIVTRVSGILSEHAKASGFVPVGLELGFGGSGPLPPLTFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDRGLDLAEVYYGLALQMLTYLDLSITHSADWLGMKATPAGVLYFHIHDPMIQASLPMGLDEIEQEIFKKFKMKGL
LLGDREAISLMDTTLEEGRSNIVNAGLKKDGSLRSDSAAVSEQDFHLLTDHVRRTFEQAGEAITDGLVSITPYKLKDKTP
CTYCAFKSVCQFDESLKENEYRSLKAEKDGTILDWLKKEADDDANS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=657418 MG974_RS05390 WP_044053080.1 1036017..1039517(+) (addB) [Bacillus velezensis strain 504]
TTGGGTGTTGAGTTTTTAGTAGGCAGATCGGGGAGTGGGAAAACGAGGCTGATTATCGACAGCATTCAAGATGAACTGCG
CCGGGAGCCGTTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATATGAGCTTGCTAAAA
CGCCGGATATCGGCGGCATGATACGCGCTCAAGTGTTCAGTTTTTCGCGTTTGGCGTGGCGCGTGCTTCAGCATACCGGG
GGCATGAACCGTCCGTTTGTGACGACGACGGGTGTGCAGATGCTTTTAAGGAAACTGATTGAAGAACATAAGCACGATTT
TAAAGTGTATCAAAAAGCGAGTGACAAAGCCGGATTCACTGAGCAGGTGGAACGGATGCTGACAGAGTTTAAGCGATACT
GTATTGAGCCTGAAGACGTCCGCCGCATGGCGGAAAGCGGAACGGCGTCAGAGTATCGCGGAGAGCGGATGCTGTCTGAA
AAACTTCATGACTTAGGCATTTTATATCAGCAGATGGAAAGAAGCCTCGCCGGACACTATTTGCATTCGGAGGATTATTT
GACGCTGCTCGCTCAGCAGCTTCCTTTGGCGGACGTTGTAAAAGGCGCCCGTGTTTACGTCGACGGATTCTATCAATTTA
CGCCGCAGGAATTAAGGGTGCTGGAGCAGCTGATCATGCATGCTGAACATGTCACATTCTCGCTGACGGCGGACAGCCCT
TCGGCGGAACAGGCACCGGATGAGCTCGATCTGTTCAGAATGACGGGAAGCACATACTATAAGCTTTATCAGACGGCAAA
GGAATTGAATGCCGATATTTCCTGCAAGGAACTGCATGGCACGAAGCGGCATCGGCATGTGCCGGAGCTTGCCTGCATAG
AGTCGCAGTACGATGTGCGCCCCGCCGCGGCGTATACCGGCGGAAAGGAAGCTTTTACCGTCATGCAGGCGCAAAACAGA
AGAGCTGAGCTCGAAGGTATTGCCCGGGAAATTCAATCGCTTGTCCGGGACGGAGGATACCGGTACAAAGATATGGCGAT
TCTCATCCGTCAGCCGGAAGATTATAAAGATTTGCTGAAGGAAGTGTTTGCCGATTACGGGCTTCCTTATTTTATTGACG
GAAAAGCTTCTATGCAGCATCACCCGCTTATTGAATTCATCCGTTCAAGCCTGGATGTCGTGAAGGGGAACTGGAGGTAT
GAAGCGGTGTTCCGCTGTGCGAAAACCGAACTGTTATTCCCGCTTGATCAGCCGGAGCAGAAAATAAGAGAGCAGGTAGA
TCAGCTTGAAAATTACTGTATCGCCTACGGCATTAAAGGGGAGCGCTGGACGAACGGCGAGCGGTTTGTATACAGGCGCT
TCGTATCGCTCGATGAGGATTTCGCGCAGACGGATCAGGAAATCGAGATGGAACACATGCTGAATGAGACGAGAGAGTGG
ATAGCTGCACCCCTTATCCGGCTGCAGAACCGGATGAAAAAAGCGAAAACGGTTCAAAGCATGGCGGAGGCTCTTTATCT
CTTTTTGGAAGATACCGATGTTCCCCTGAAGCTGGACCGGAAAAGGCAGCGGGCCGAAGAGGCCGGAAACATGATCGAAG
CGCAGCAGCACGGCCAGGCGTGGGATGCCGTTATCCAGCTTCTCGAGGAGTTTGCCGGCATGATGGGTGAAGATGAAATA
TCTTTGGCGCTGTTCCAGCAAATGCTTGAAACGGGAACGGAATCGCTTCATTTTTCTTTAATTCCTCCTGCGCTTGACCA
AGTATTTGTAGGGAATATGGATCTCTCAAGAATATACGGCACGTCCTGCACATTCGTCATCGGGGCCAATGACGGCGTTC
TTCCGGCGCGCCCGGATGAAAACGGCGTGCTGTCTGATGATGACCGGGAGTGGCTGAAAGCGGTCGGAGTGGAGCTGTCT
TCCGCGGGGCGTGAGCGGCTGCTTGATGAGCATTTTCTCATCTATATGGCGCTGTCGAGTCCGTCTGACCGTCTTTATGT
GTCATACCCGATTGCCGATGCGGAAGGAAAAACGCTCCTGCCGTCAATCGTTGTAAACAGGCTTGGCGAGCTGTTTCCCG
ATCATCAGGAAAAGCTGTCAGCGGCTGATCCCGAGCAGGTAAGCGAAGAAGAGCAGCTTCAGTATCTCGTCAATAAACAG
GTGGCTCAGACGTATACGGCAAGTCAGCTCAGGCTGTGGACAAGAGAGTACGAGATCAGTGACGTATGGTGGAGCGCATA
TAATGTGCTGATGAAAGAGTCTGATCACCAGCGTGCGAAAAAGCTGTTTTCAAGCCTGTTTTTCCGCAATGAGGCGAAGC
GTCTTGAACGGCCTGTTTCGCGCCAGCTTTACGGCGAGCATATTAAAGGAAGCGTCTCCCGGATGGAGGCGTTTAATGCC
TGCCAGTTTTCTCATTTTGCATCTCACGGGCTGCAATTAAAAGAGCGGCAGTTTTTCAAGCTGGAAGCGCCGGATATCGG
CCAGCTGTTTCACTCCAGTCTGAAGCTGATTTCAGACCGGCTTCGTGAGCAGAAGCTGGAATGGCGCGATCTGACGAAAG
ATCAGTGCCGGCATTTCTCCTATGAAGCGGTGGAACGGCTTGCGCCAAAGCTGCAAAAAGAGATTCTGCTCAGCTCAAAC
AGACACTTTTATGTGAAGGAAAAACTGCAGAAGATCGTCACGCGTGTTTCTGGCATATTAAGCGAGCATGCGAAGGCGAG
CGGTTTCGTCCCGGTCGGTCTTGAACTCGGTTTCGGCGGAAGCGGACCGCTGCCTCCGCTCACTTTCACGCTGAAAAACG
GCTGTACGATGGAACTCGTCGGACGGATCGACCGCGTGGATAAAGCGGAAAGTTCAAAAGGCCTGCTGCTGAGAATCGTT
GATTATAAATCAAGCGACAGGGGGCTTGATCTGGCTGAGGTGTATTACGGGCTCGCTCTGCAAATGCTCACATATCTTGA
CTTATCGATTACACATTCTGCGGATTGGCTCGGCATGAAAGCGACCCCGGCAGGCGTTTTGTATTTCCATATCCATGATC
CGATGATTCAGGCCAGTCTGCCGATGGGGCTTGATGAAATCGAGCAGGAAATTTTCAAGAAATTTAAAATGAAAGGCCTT
CTCCTCGGTGACCGGGAGGCGATCAGCCTGATGGACACGACTTTGGAGGAAGGGCGTTCAAATATCGTCAATGCCGGTTT
GAAAAAGGACGGGTCTTTAAGATCCGACTCAGCGGCGGTCAGCGAACAGGACTTTCATCTGCTGACAGACCATGTGCGGC
GGACATTTGAACAGGCGGGCGAAGCGATAACGGACGGGCTTGTGTCCATTACCCCGTATAAGCTGAAAGATAAGACGCCT
TGTACGTACTGTGCGTTTAAATCGGTTTGCCAGTTTGATGAGTCGCTTAAGGAAAATGAATACCGCAGCCTGAAAGCGGA
AAAAGACGGCACCATACTGGATTGGCTGAAAAAGGAGGCGGATGATGATGCAAATTCCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(791-1131)

UvrD-like helicase C-terminal domain (UvrD_C)

(309-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

83.362

100

0.834