Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   MGJ28_RS24100 Genome accession   NZ_CP092387
Coordinates   4580753..4583095 (-) Length   780 a.a.
NCBI ID   WP_241096594.1    Uniprot ID   -
Organism   Priestia megaterium strain BP01R2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4575753..4588095
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MGJ28_RS24075 (MGJ28_24080) - 4576146..4576322 (-) 177 WP_013059305.1 hypothetical protein -
  MGJ28_RS24080 (MGJ28_24085) - 4576489..4577373 (+) 885 WP_241096593.1 N-acetylmuramoyl-L-alanine amidase -
  MGJ28_RS24085 (MGJ28_24090) dtd 4577364..4577825 (-) 462 WP_016765630.1 D-aminoacyl-tRNA deacylase -
  MGJ28_RS24090 (MGJ28_24095) - 4577841..4580039 (-) 2199 WP_013059308.1 RelA/SpoT family protein -
  MGJ28_RS24095 (MGJ28_24100) - 4580225..4580737 (-) 513 WP_013059309.1 adenine phosphoribosyltransferase -
  MGJ28_RS24100 (MGJ28_24105) recJ 4580753..4583095 (-) 2343 WP_241096594.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  MGJ28_RS24105 (MGJ28_24110) secDF 4583236..4585473 (-) 2238 WP_241096595.1 protein translocase subunit SecDF -
  MGJ28_RS24110 (MGJ28_24115) - 4585611..4585904 (-) 294 WP_016765633.1 post-transcriptional regulator -
  MGJ28_RS24115 (MGJ28_24120) spoVB 4586029..4587579 (+) 1551 WP_034669037.1 stage V sporulation protein B -

Sequence

Protein


Download         Length: 780 a.a.        Molecular weight: 87989.60 Da        Isoelectric Point: 4.6994

>NTDB_id=657160 MGJ28_RS24100 WP_241096594.1 4580753..4583095(-) (recJ) [Priestia megaterium strain BP01R2]
MLQSKKRWNVQECDEQLVNEFVENLNITPLVASLLLNRGMTTVEAAREFLHVDAQTFHDPFLLHDMDKAVARIQQAIDHN
EKIMIYGDYDADGVSSTSVMLSALQQLGADTDFYIPNRFTEGYGPNKAAFDKINTGGYQLVITVDTGISAVEETAYAREL
DFDLIITDHHEPGPILPDAYAIIHPKLPGSTYPFKELAGVGVAFKLAHALLGELPEHLLELAVIGTIADLVPLVDENRLI
ARKGLDYLKLTKRTGIEALLSVCGVKREEITEDTIGFAMAPRINAVGRLQDADPAVHLLMTTNAEEAKGLAKEIDALNKV
RQQIVNDITEEAVEFVENMYPPDENSVLIVEGEGWNAGVVGIVASRLVEKFYRPTIVLSIDSENRIAKGSARSIEGFDLF
ANLSKCRDILPHFGGHPMAAGMTLNSDDVEELRTRLNEQASNVLTEQDFIPITSVDTVCTTEEITLENIEQMSALAPFGM
HNPKPKVLIKDVHTTSMRKIGANKNHLKLVFEDSDVSVDAVGFGLGYILDEVSPIAKVSLVGELSINEWNNMRKPQLMVE
DISVDEWQMFDYRSAVDVKRFFQQVFTDQMKVVAFHASTIDKYKSLLPMEHVVLIQDEEHAKECMFAGEQIVLLDLPPLQ
KWLECLFVQGIPSRIYAIFLTEEDHYFDTIPTRDHFKWFYGFLAKKGPFDLKRYKEDIAIHKGWSKETIDFMAKVFFELE
FVTINNGLISLVKNSAKRDLIESATYRQKQQQIEIEKNFIYTSYVQLKNQFQDIFKRSIE

Nucleotide


Download         Length: 2343 bp        

>NTDB_id=657160 MGJ28_RS24100 WP_241096594.1 4580753..4583095(-) (recJ) [Priestia megaterium strain BP01R2]
ATGTTACAGTCAAAAAAACGTTGGAATGTTCAAGAATGTGATGAACAACTTGTTAATGAATTTGTAGAAAACCTCAATAT
TACACCTCTAGTAGCTTCGTTATTACTTAATAGAGGCATGACAACAGTAGAAGCGGCACGTGAGTTTTTACACGTTGATG
CACAAACGTTCCACGATCCGTTTTTATTGCACGATATGGACAAAGCGGTAGCTCGTATTCAGCAAGCAATAGATCATAAT
GAAAAAATTATGATCTATGGAGATTACGATGCGGACGGCGTCAGCAGTACTTCTGTTATGTTATCTGCTTTACAGCAGCT
CGGCGCTGATACTGATTTTTATATTCCTAATCGCTTTACAGAAGGTTATGGGCCGAATAAGGCAGCATTTGATAAAATAA
ACACAGGCGGCTATCAGCTGGTGATTACGGTAGACACAGGTATTTCGGCAGTCGAAGAAACGGCGTATGCACGTGAATTA
GATTTTGATTTAATTATTACGGATCACCACGAGCCGGGTCCGATTTTACCGGATGCGTATGCAATTATTCATCCAAAGCT
GCCGGGAAGTACATATCCATTTAAAGAACTGGCGGGTGTAGGAGTGGCTTTTAAACTCGCTCATGCGCTGTTAGGGGAGC
TTCCGGAACATTTGTTAGAACTAGCAGTGATTGGGACGATTGCTGACTTGGTTCCTTTAGTCGACGAAAACCGCTTAATT
GCTCGCAAAGGTCTTGACTATCTCAAGCTCACGAAGCGCACTGGAATTGAAGCGCTGCTGAGCGTATGTGGAGTAAAACG
CGAAGAAATTACTGAAGATACAATTGGATTTGCTATGGCTCCTCGCATTAATGCAGTAGGACGTCTTCAAGATGCTGATC
CAGCTGTTCACTTGCTTATGACAACAAATGCTGAAGAAGCAAAAGGTTTAGCTAAAGAAATTGATGCGTTAAACAAGGTA
AGGCAGCAAATCGTAAACGATATTACGGAAGAAGCCGTTGAATTTGTTGAAAATATGTATCCTCCGGATGAAAACAGCGT
TCTTATTGTAGAGGGAGAAGGATGGAATGCAGGTGTTGTTGGAATTGTCGCTTCTAGGCTCGTAGAAAAGTTCTACAGAC
CAACAATTGTACTAAGTATTGACTCTGAAAACCGTATTGCCAAAGGTTCAGCAAGAAGTATCGAAGGCTTTGACTTATTC
GCGAACTTATCTAAATGCCGTGACATTTTGCCGCATTTTGGAGGGCATCCAATGGCAGCCGGGATGACGTTAAACAGCGA
TGATGTAGAAGAGCTGCGCACACGTCTTAATGAACAAGCAAGCAATGTGTTAACCGAACAGGATTTTATTCCAATTACAT
CAGTTGATACAGTATGCACGACAGAGGAAATTACGCTGGAAAACATTGAACAAATGAGTGCGCTTGCTCCATTTGGTATG
CATAACCCGAAGCCAAAAGTTCTCATTAAAGATGTACATACAACATCTATGCGGAAAATTGGCGCTAATAAAAATCATTT
AAAACTGGTGTTTGAAGACAGCGATGTATCGGTTGATGCAGTAGGGTTTGGGTTAGGCTATATATTAGATGAAGTATCTC
CTATAGCTAAAGTATCGTTAGTAGGTGAACTATCTATTAATGAGTGGAATAATATGCGCAAGCCGCAGCTGATGGTAGAA
GATATTTCTGTAGATGAATGGCAGATGTTTGATTATCGCAGTGCAGTTGATGTCAAACGTTTCTTTCAGCAGGTATTTAC
GGATCAAATGAAAGTTGTTGCTTTTCACGCTTCAACAATTGATAAATACAAGTCGCTGCTGCCGATGGAGCATGTTGTTC
TTATTCAAGATGAAGAACATGCAAAAGAATGTATGTTTGCAGGGGAACAAATCGTTTTATTAGATTTGCCTCCTTTACAG
AAGTGGCTTGAATGTTTGTTTGTCCAAGGTATCCCTTCTAGAATATACGCTATTTTCTTAACGGAAGAAGACCATTATTT
TGATACAATCCCAACTCGCGATCATTTTAAATGGTTTTACGGCTTTTTAGCGAAAAAGGGTCCATTTGATTTAAAACGCT
ACAAAGAAGATATAGCGATTCATAAGGGCTGGTCAAAAGAAACGATTGATTTTATGGCAAAGGTGTTTTTTGAGTTAGAA
TTTGTTACAATAAACAATGGCTTAATTTCGTTAGTGAAAAATTCTGCGAAACGTGATTTAATAGAATCTGCGACTTATCG
CCAAAAGCAGCAGCAGATTGAAATAGAAAAAAACTTTATTTATACGTCATATGTACAGCTTAAAAATCAATTTCAAGATA
TTTTTAAGCGTTCGATTGAATGA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(305-439)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-770)

DHH family (DHH)

(82-226)

RecJ OB domain (RecJ_OB)

(455-561)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

55.115

100

0.553