Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   MID02_RS13040 Genome accession   NZ_CP092383
Coordinates   2716368..2718728 (-) Length   786 a.a.
NCBI ID   WP_101312017.1    Uniprot ID   -
Organism   Bacillus velezensis strain SF327     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2711368..2723728
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MID02_RS13020 (MID02_13020) - 2711443..2713005 (+) 1563 WP_015387900.1 SH3 domain-containing protein -
  MID02_RS13025 (MID02_13025) dtd 2713032..2713475 (-) 444 WP_007408194.1 D-aminoacyl-tRNA deacylase -
  MID02_RS13030 (MID02_13030) relA 2713488..2715692 (-) 2205 WP_003152716.1 GTP diphosphokinase -
  MID02_RS13035 (MID02_13035) - 2715850..2716362 (-) 513 WP_003152714.1 adenine phosphoribosyltransferase -
  MID02_RS13040 (MID02_13040) recJ 2716368..2718728 (-) 2361 WP_101312017.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  MID02_RS13045 (MID02_13045) - 2718784..2719110 (-) 327 WP_003152709.1 lipopolysaccharide assembly LapA domain-containing protein -
  MID02_RS13050 (MID02_13050) - 2719174..2719671 (-) 498 WP_003152707.1 cation:proton antiporter regulatory subunit -
  MID02_RS13055 (MID02_13055) secDF 2719803..2722022 (-) 2220 WP_003152704.1 protein translocase subunit SecDF -
  MID02_RS13060 (MID02_13060) - 2722059..2722355 (-) 297 WP_003152702.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 786 a.a.        Molecular weight: 87424.82 Da        Isoelectric Point: 4.9218

>NTDB_id=657049 MID02_RS13040 WP_101312017.1 2716368..2718728(-) (recJ) [Bacillus velezensis strain SF327]
MLASKMRWEIQQPDQEKVKSLTEQLDITPLVASLLVKRGFDTAESARLFLHTKDADFYDPYELKGMREAVDRIKLAVSQG
EPIMIYGDYDADGVTSTSVMLTALRQLTADVDFYIPDRFTEGYGPNEQAFRSIKERGFSLIITVDTGIAAVHEAEVAKEL
GLDVIITDHHEPGPELPDVFAIIHPKQPGCGYPFKELAGVGVAFKLAHALTGSLPEAMLDLAAIGTVADLVPLHDENRLI
ASLGIERLRTTNRPGLKALIKLSGGDIGQADEETIGFQVAPRLNAVGRIEQADPAVHLLMTEDQFEAEELASDIDRLNKE
RQKMVKTMTEEAVEMVQQQGGNPSAIVVAKAGWNPGVVGIVASKLAERFYRPAVVLGIDEEQGIAKGSARSIRGFNLFTS
LSQCRDILPHFGGHPMAAGMTLKAEDVAELRDRLNQMAEETLTEEDFIPVQEVDLVCGVEDITVESITELNMLSPFGMLN
PKPHVMVKNAVLDDVRLIGANKNHVKMTVKSDSAVLDCIGFNKGELEAGVVPGSTISIVGEMSINEWNNRKKPQLMIKDA
AVPEWQLFDIRGKRTWEDTVTALPKEKRVLISFNEKTAQKLREKELTGELAEITSESEAEALQLDGKYAVFLDVPPSADR
LQHLLDGKKPERIYFVFINHEEHFLSSFPSRNDFKWFYAFLLKRGAFDFGKHGSELARHRGWSIDTINFMTKVFFDLGFV
KIEDGVLSVVSGAKKRDLADSQTYQVKQQLMELDQKLNYSAAHELKEWLNKLMNQGSEAYESTRRT

Nucleotide


Download         Length: 2361 bp        

>NTDB_id=657049 MID02_RS13040 WP_101312017.1 2716368..2718728(-) (recJ) [Bacillus velezensis strain SF327]
ATGTTAGCGTCAAAAATGCGCTGGGAAATCCAGCAGCCGGATCAGGAAAAAGTCAAATCACTCACAGAACAATTGGACAT
AACACCTCTTGTGGCATCACTGCTTGTAAAAAGAGGTTTTGATACTGCCGAAAGCGCAAGGCTGTTTTTACATACAAAGG
ATGCAGACTTTTATGACCCGTATGAATTAAAGGGAATGCGGGAAGCAGTTGATCGGATAAAACTGGCGGTTTCTCAAGGG
GAACCGATTATGATTTACGGAGATTACGATGCAGACGGCGTTACAAGCACGTCAGTGATGCTGACGGCACTCAGACAGCT
GACGGCGGACGTTGACTTTTATATTCCCGACCGTTTTACAGAAGGCTACGGCCCGAATGAACAAGCTTTCCGTTCCATAA
AAGAGCGGGGATTTTCGCTGATCATTACAGTGGATACGGGAATCGCCGCCGTCCATGAAGCTGAAGTCGCAAAAGAGCTG
GGCTTAGACGTCATTATTACCGACCACCATGAGCCGGGACCCGAGCTTCCTGACGTATTTGCGATCATCCACCCGAAGCA
GCCGGGATGCGGCTATCCGTTTAAAGAATTGGCCGGCGTCGGTGTGGCGTTTAAGCTTGCCCACGCGCTTACGGGTTCTT
TGCCGGAAGCTATGCTGGACTTGGCGGCAATCGGTACGGTTGCAGATCTGGTGCCTCTTCATGATGAAAACAGATTAATT
GCGTCACTCGGAATTGAAAGGCTTCGTACGACAAATCGGCCGGGCCTCAAAGCTCTGATTAAGCTTTCCGGCGGAGATAT
CGGACAAGCTGATGAGGAAACGATTGGATTTCAGGTCGCACCGAGGCTTAATGCCGTCGGAAGAATAGAACAGGCCGATC
CCGCTGTGCACCTGCTGATGACCGAAGATCAGTTTGAAGCGGAGGAACTGGCTTCGGATATTGACCGGCTGAATAAAGAG
CGGCAGAAAATGGTGAAAACCATGACCGAAGAGGCTGTTGAAATGGTACAGCAGCAAGGCGGAAACCCGTCTGCCATTGT
AGTGGCAAAAGCCGGCTGGAATCCGGGCGTCGTCGGTATTGTCGCATCGAAGCTGGCTGAACGTTTTTACCGGCCCGCTG
TTGTGCTTGGGATAGATGAGGAGCAGGGAATCGCCAAAGGGTCGGCGCGAAGCATCAGAGGTTTCAACCTTTTTACGAGC
CTTTCACAGTGCAGAGACATTCTTCCTCATTTCGGCGGGCATCCGATGGCGGCCGGAATGACGCTGAAGGCTGAAGATGT
GGCGGAATTAAGGGACAGGCTGAATCAAATGGCTGAAGAAACCCTTACGGAAGAGGATTTTATTCCCGTTCAGGAGGTTG
ATCTTGTCTGCGGCGTTGAAGACATAACCGTTGAAAGCATTACAGAATTGAATATGCTGTCGCCGTTCGGCATGCTTAAC
CCTAAACCGCATGTGATGGTGAAAAATGCCGTGCTTGATGATGTGCGCCTGATCGGAGCGAATAAAAACCACGTGAAAAT
GACGGTGAAAAGTGATTCCGCCGTCCTTGACTGCATCGGCTTTAACAAAGGAGAGCTGGAAGCCGGAGTTGTTCCCGGAT
CAACGATTTCCATTGTCGGCGAGATGTCCATTAATGAATGGAACAACAGAAAGAAGCCCCAGCTGATGATTAAAGATGCT
GCGGTGCCGGAATGGCAGCTTTTTGATATTCGAGGCAAGCGGACGTGGGAAGATACGGTCACCGCACTGCCGAAGGAAAA
ACGGGTGCTCATCAGCTTTAACGAAAAAACCGCTCAGAAGCTCCGTGAAAAAGAGCTGACGGGCGAGCTTGCCGAGATCA
CTTCGGAATCCGAGGCGGAAGCTCTCCAGCTTGACGGAAAATATGCCGTTTTTCTGGATGTGCCGCCGTCAGCGGATCGC
CTGCAGCATTTGCTTGACGGAAAGAAGCCGGAACGGATTTATTTTGTTTTTATCAATCATGAAGAACATTTCCTGTCCTC
ATTTCCATCGCGGAATGATTTCAAGTGGTTTTACGCATTTCTCTTGAAAAGGGGCGCTTTTGACTTCGGCAAGCATGGTT
CAGAGCTTGCGAGACACAGAGGATGGTCAATTGACACAATCAATTTCATGACAAAGGTGTTTTTTGATCTCGGTTTTGTT
AAAATAGAAGATGGTGTGCTGTCTGTCGTGAGCGGTGCGAAAAAACGCGACCTGGCAGATTCGCAAACCTATCAGGTAAA
ACAGCAGCTGATGGAATTAGACCAGAAGCTCAATTATTCCGCAGCCCATGAGCTGAAGGAATGGCTGAATAAGCTGATGA
ACCAAGGTTCAGAGGCTTATGAAAGTACGAGGAGGACATAG

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(315-437)

DHH family (DHH)

(83-226)

RecJ OB domain (RecJ_OB)

(453-559)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-769)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

81.807

100

0.818