Detailed information NTG0656829

Bioinformatically predicted

Overview

• Name: addB
• Type: Machinery gene
• Locus tag: MID01_RS05635
• Genome accession: NZ_CP092369
• Coordinates: 1094588..1098088 (+)
• Length: 1166 a.a.
• NCBI ID: WP_015715638.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain ZW
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1089588..1103088

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MID01_RS05615 (MID01_05615)	yhjO	1090446..1091651 (-)	1206	WP_015715635.1	MFS transporter	-
MID01_RS05620 (MID01_05620)	yhjP	1091765..1093492 (+)	1728	WP_239795070.1	SgrR family transcriptional regulator	-
MID01_RS05625 (MID01_05625)	yhjQ	1093522..1093848 (-)	327	WP_009966950.1	four-helix bundle copper-binding protein	-
MID01_RS05630 (MID01_05630)	yhjR	1093967..1094404 (-)	438	WP_015715637.1	ferritin family protein	-
MID01_RS05635 (MID01_05635)	addB	1094588..1098088 (+)	3501	WP_015715638.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
MID01_RS05640 (MID01_05640)	addA	1098075..1101773 (+)	3699	WP_043940091.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
MID01_RS05645 (MID01_05645)	sbcD	1101845..1103020 (+)	1176	WP_041851011.1	exonuclease subunit SbcD	-

Sequence

Protein

• Length: 1166 a.a.
• Molecular weight: 134640.62 Da
• Isoelectric Point: 5.3800

>NTDB_id=656829 MID01_RS05635 WP_015715638.1 1094588..1098088(+) (addB) [Bacillus subtilis strain ZW]
MGAEFLVGRSGSGKTKLIIDSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDMGGMIRAQVFSFSRLAWRILQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKSGFTAQVERMLTEFKRYCLEPEDIRRMAESGTASEYRGERVLSE
KLHDLSILYQQMEKSLADQYLHSEDYLTLLAEHIPLAEDIKGAHIYVDGFYQFTPQEFRVLEQLMVHAEHITFSLTADKP
SYEREPHELELFRMTGKTYYRLHQKAKELNLDITYKELSGTERHTKTPELAHLEAQYEARPAVPYAEKQEALTVMQAANR
RAELEGIAREIHALVREKGYRYKDVAILARQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLIEFIRSSLDVLKGNWRY
EAVFRCVKTELLFPLNEPKAKVREQVDQLENYCIAYGIKGDRWTKGDRFQYRRFVSLDDDFAQTDQEIEMENMLNDTRDW
IVPPLFQLQKRMKKAKTVQEKAEALYRYLEETDVPLKLDQERQRAEDDGRIIEAQQHQQAWDAVIQLLEEFVEMMGDDEI
SLDLFQQMIEAGAESLTFSLIPPALDQVFVGNMDLSRMYGTSCTFVLGANDGVLPARPDENGVLSDDDREWLKTIGVELS
SGGRERLLDEHFLIYMAFSSPSDRLYVSYPIADAEGKTLLPSMIVKRLEELFPYHKERLLTNEPEQVSDEEQLMYVVNQS
VAQSFTASQLRLWTREYDISDVWWSTYNVLMSEPDRLQSKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLDWRDLTKEQCELFSYDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFQLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMRATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEVVRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFDLLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCAFKSVCQFDESLEENEYRPLKAEKDKTILEWIKKEADGNEHS

Nucleotide

• Length: 3501 bp

>NTDB_id=656829 MID01_RS05635 WP_015715638.1 1094588..1098088(+) (addB) [Bacillus subtilis strain ZW]
TTGGGAGCAGAGTTTTTAGTAGGCAGGTCCGGGAGTGGAAAAACGAAGCTGATCATCGACAGCATTCAGGATGAATTGCG
CCGGGCTCCATTCGGGAAGCCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTGCTAAAA
CGCCAGATATGGGCGGGATGATACGCGCTCAAGTATTCAGTTTTTCACGATTGGCCTGGCGCATCCTCCAGCATACGGGA
GGAATGAGCAGGCCGTTTCTGACGAGCACCGGCGTACAAATGCTCCTGCGGAAGCTCATTGAGGAGCATAAACAGGAGTT
CAAAGTCTATCAAAAAGCGAGTGACAAAAGCGGGTTTACCGCACAGGTTGAGCGGATGCTGACAGAGTTTAAGCGCTATT
GTCTGGAACCGGAGGATATCCGCCGGATGGCGGAAAGCGGAACGGCTTCCGAGTATCGCGGAGAACGAGTTTTATCTGAA
AAGCTCCATGACTTATCAATTCTGTATCAGCAAATGGAAAAAAGCCTCGCAGACCAATATCTTCACTCTGAGGATTATTT
GACATTGCTGGCAGAGCATATCCCGCTTGCGGAAGATATAAAAGGCGCCCATATCTATGTGGATGGCTTTTATCAGTTTA
CACCGCAGGAATTCAGGGTGTTGGAGCAGCTTATGGTTCATGCGGAGCATATCACGTTTTCGCTCACAGCGGACAAGCCG
TCATATGAGCGGGAGCCGCATGAACTGGAATTGTTCAGAATGACGGGGAAAACCTATTACCGCCTGCATCAGAAGGCGAA
GGAACTGAATTTGGACATTACGTATAAAGAGCTGAGCGGGACTGAGCGGCATACAAAGACGCCGGAATTGGCGCATCTAG
AGGCACAGTATGAAGCGCGTCCGGCCGTTCCATACGCAGAAAAACAAGAAGCCCTTACTGTGATGCAGGCCGCAAACAGA
CGGGCTGAGCTGGAAGGCATTGCCCGGGAAATTCACGCCCTAGTCAGAGAGAAGGGATATCGCTATAAGGATGTAGCCAT
TCTTGCGCGCCAGCCGGAAGACTACAAGGATATGGTGAAGGAAGTTTTCGCAGATTACGAGATTCCTTATTTCATTGACG
GAAAAGCATCTATGCTGAACCATCCGTTAATTGAATTTATCCGGTCAAGCCTTGATGTTCTGAAAGGGAATTGGCGTTAT
GAAGCGGTGTTTCGCTGCGTGAAAACCGAACTGCTATTCCCGCTCAATGAACCGAAGGCCAAAGTGAGAGAACAGGTCGA
TCAGCTCGAAAATTACTGTATCGCCTATGGTATTAAAGGCGACCGCTGGACAAAGGGCGATCGGTTCCAATACAGGCGTT
TTGTGTCATTGGATGATGATTTTGCGCAGACAGATCAGGAAATCGAAATGGAAAACATGTTGAATGACACCCGCGATTGG
ATTGTTCCGCCGCTTTTTCAGCTTCAAAAACGCATGAAAAAAGCGAAGACGGTTCAAGAGAAGGCGGAGGCGCTCTATCG
TTATTTAGAAGAGACGGATGTGCCGCTGAAGCTGGATCAGGAAAGGCAGCGTGCTGAGGATGACGGCAGAATCATTGAAG
CGCAGCAGCATCAGCAGGCGTGGGACGCAGTCATTCAGCTGCTTGAGGAGTTTGTTGAAATGATGGGAGATGATGAGATT
TCTCTTGATTTGTTTCAGCAAATGATAGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCA
GGTGTTTGTCGGCAATATGGATTTGTCCAGAATGTATGGCACCTCCTGCACCTTTGTGCTCGGGGCAAACGACGGCGTTC
TGCCGGCACGCCCTGATGAAAACGGAGTCCTGTCGGATGATGACCGGGAATGGCTGAAAACGATTGGGGTTGAGCTATCC
TCAGGCGGACGGGAGCGTTTGCTTGATGAGCATTTCCTCATCTACATGGCATTTTCAAGTCCGTCTGACCGGCTTTACGT
ATCGTATCCGATTGCTGATGCGGAAGGAAAAACGCTTTTGCCGTCGATGATCGTTAAGCGGCTGGAAGAACTGTTTCCAT
ATCATAAGGAGCGCCTGTTAACAAATGAACCTGAACAGGTCAGCGATGAGGAACAGCTGATGTATGTCGTGAATCAAAGC
GTGGCACAGTCCTTTACCGCGAGCCAGCTCAGGTTATGGACACGGGAATACGACATCAGCGACGTCTGGTGGAGCACGTA
CAATGTGCTGATGAGTGAGCCGGACAGGCTGCAGTCGAAAAAGCTGTTCTCAAGCCTGTTTTTCCGGAATGAAGTGAAGC
AGCTTGAACGCAGCGTGTCGAGACAGCTCTATGGTGAACGCATTCAGGGCAGTGTATCGAGAATGGAAACCTTTAACGCA
TGCCCGTTTTCCCATTTTGCGTCACACGGGCTGCATTTGAAGGAACGGCAATTCTTCAAGCTTGAAGCACCGGATATCGG
CCAGCTGTTTCATTCCAGCTTAAAGCTGATTTCTGACAGGCTGCGTGAGCAAAAGCTGGATTGGCGCGATTTAACGAAAG
AGCAATGCGAGCTGTTTTCCTATGATGCGGTAGAGCGGCTGGCACCGAAACTGCAAAAGGAAATTCTGCTCAGCTCAAAC
CGGCATTATTATGTGAAGGAAAAACTGCAAAAAATTGTGACACGTGTATCCGGCATTTTAAGCGAGCATGCGAAAGCGAG
CGGATTCGTACCGATCGGGCTTGAACTGGGCTTTGGAGGAAAAGGGCCGCTTCCGCCGCTGACCTTTCAACTGAAAAACG
GCTGTACGATGGAACTCGTCGGGCGAATTGACCGTGTTGATAAGGCTGAAAGCTCAAAAGGCCTGCTCCTCAGGATTGTC
GATTATAAATCAAGCGACAAAGGCCTTGACTTAGCGGAAGTATATTACGGATTGGCACTGCAAATGCTGACGTACCTTGA
TTTATCGATTACACATTCAGCTGACTGGCTCGGGATGAGGGCGACGCCTGCCGGAGTGCTGTATTTCCATATTCATGACC
CGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTG
CTCCTCGGTGATCAGGAAGTGGTTCGCCTCATGGATACAACCCTTCAAGAGGGACGGTCAAATATCATAAACGCCGGCTT
GAAAAAAGACGGATCTCTTAGATCAGACTCGGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCC
GCACCTTCCAGGAAGCGGGCGAACAAATCACCGACGGGCGCGTATCCATTGAGCCGTACAAAATGAAGAACAAGACGCCG
TGCACATACTGTGCGTTCAAATCAGTATGCCAATTTGATGAATCACTGGAAGAAAACGAGTATCGCCCATTAAAGGCTGA
AAAGGACAAGACAATACTTGAGTGGATAAAAAAGGAGGCGGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (305-668)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (790-1131)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addB	Bacillus subtilis subsp. subtilis str. 168	99.485	100	0.995