Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MIS45_RS06165 Genome accession   NZ_CP092361
Coordinates   1252304..1253488 (-) Length   394 a.a.
NCBI ID   WP_283397256.1    Uniprot ID   -
Organism   Wielerella bovis strain CCUG 44465     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1247304..1258488
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIS45_RS06145 (MIS45_06145) grxB 1247460..1248110 (-) 651 WP_249449887.1 glutaredoxin 2 -
  MIS45_RS06150 (MIS45_06150) - 1248185..1250071 (-) 1887 WP_249449888.1 lytic transglycosylase domain-containing protein -
  MIS45_RS06155 (MIS45_06155) - 1250201..1250809 (-) 609 WP_249449889.1 nitroreductase family protein -
  MIS45_RS06160 (MIS45_06160) - 1251035..1251721 (+) 687 WP_249449890.1 sulfite exporter TauE/SafE family protein -
  MIS45_RS06165 (MIS45_06165) pilU 1252304..1253488 (-) 1185 WP_283397256.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MIS45_RS06170 (MIS45_06170) - 1253661..1254431 (-) 771 WP_249449891.1 ADP-ribosylglycohydrolase family protein -
  MIS45_RS06175 (MIS45_06175) folP 1254499..1255344 (-) 846 WP_249449892.1 dihydropteroate synthase -
  MIS45_RS06180 (MIS45_06180) - 1255365..1255799 (-) 435 WP_249449893.1 META domain-containing protein -
  MIS45_RS06185 (MIS45_06185) - 1255835..1256203 (-) 369 WP_249441703.1 hemerythrin domain-containing protein -
  MIS45_RS06195 (MIS45_06195) - 1256916..1257710 (+) 795 WP_249441702.1 c-type cytochrome -

Sequence

Protein


Download         Length: 394 a.a.        Molecular weight: 43958.39 Da        Isoelectric Point: 5.6463

>NTDB_id=656775 MIS45_RS06165 WP_283397256.1 1252304..1253488(-) (pilU) [Wielerella bovis strain CCUG 44465]
MSALPPVNEEMKKFIHSLLTHMVKNKGSDLFITAGFPPAMKLDGKLTRLSDKPLTAEHTMQIARSIMDDKQAEEFLNTNE
CNFAISLAGVSRFRINAMIQRGAAALVCRVITSDIPKFEDLKLPPVLRDVVMEKRGLVIFVGGTGSGKSTSLAAMIDYRN
ENSFGHIITIEDPIEYVHPHKNCIVSQREVGVDTENWFAALKNTLRQAPDVILIGEIRDRETMDYALAFAETGHLCMATL
HANNSNQALDRIINFFPEERRTQLLTDLSLNLKGFISQRLIPRKTGKGRSAAVEILLNSPLISELILKGEVHGIKEIMAK
SRELGMQTFDQALFDLYEDDMISYDDAIKNADSANDLRLQIKLNSKKNATAGEDGALDGLSIMDYQHEQPDEQQ

Nucleotide


Download         Length: 1185 bp        

>NTDB_id=656775 MIS45_RS06165 WP_283397256.1 1252304..1253488(-) (pilU) [Wielerella bovis strain CCUG 44465]
ATGTCCGCACTCCCACCCGTAAACGAAGAAATGAAAAAGTTCATTCACAGCTTATTGACTCACATGGTCAAAAACAAAGG
CTCGGACTTATTCATCACCGCAGGCTTCCCACCAGCCATGAAATTGGACGGCAAATTAACCCGTTTGAGCGATAAACCGC
TGACCGCCGAACACACCATGCAAATCGCGCGTTCCATTATGGACGACAAACAAGCCGAAGAATTTTTAAACACCAACGAA
TGCAACTTCGCCATCAGTTTGGCTGGTGTATCTCGTTTCCGTATCAATGCCATGATTCAACGTGGCGCAGCCGCTTTGGT
TTGCCGTGTGATTACCAGTGATATTCCCAAATTTGAAGATTTAAAATTGCCGCCTGTATTGCGCGATGTGGTTATGGAAA
AACGTGGCTTGGTGATTTTTGTTGGCGGTACAGGTTCGGGTAAATCCACATCTCTCGCTGCTATGATTGATTATCGCAAT
GAAAACAGCTTTGGTCATATCATTACCATTGAAGACCCGATTGAATATGTGCATCCACACAAAAATTGCATCGTTTCACA
ACGCGAAGTCGGCGTGGATACCGAAAACTGGTTTGCCGCATTGAAAAACACCTTGCGCCAAGCCCCAGATGTGATTTTGA
TTGGTGAGATTCGCGACCGCGAAACCATGGACTATGCGCTTGCTTTTGCCGAAACAGGACACTTGTGTATGGCAACCTTG
CATGCCAACAACTCTAACCAAGCACTTGACCGCATCATCAACTTCTTCCCCGAAGAACGCCGCACCCAATTGCTGACTGA
CTTATCTTTGAACTTAAAAGGTTTCATTTCGCAACGTTTGATTCCACGAAAAACAGGTAAAGGTCGTTCAGCAGCAGTAG
AAATTTTGTTGAACTCGCCTTTGATTTCCGAATTGATTTTGAAAGGCGAAGTGCATGGCATTAAAGAAATCATGGCGAAA
TCACGCGAATTGGGCATGCAAACCTTTGACCAAGCCTTGTTTGATTTGTATGAAGACGACATGATTTCCTATGACGATGC
GATTAAAAATGCCGACTCTGCCAACGACTTGCGTTTACAAATTAAATTGAACAGTAAGAAAAATGCCACAGCTGGCGAAG
ATGGGGCATTGGACGGCTTGTCTATTATGGATTATCAGCACGAACAGCCTGATGAGCAGCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(125-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.795

89.34

0.543
  pilU Acinetobacter baylyi ADP1

59.659

89.34

0.533
  pilU Vibrio cholerae strain A1552

55.096

92.132

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.899

90.863

0.381
  pilT Legionella pneumophila strain Lp02

43.243

84.518

0.365
  pilT Legionella pneumophila strain ERS1305867

43.243

84.518

0.365
  pilT Pseudomonas aeruginosa PAK

42.433

85.533

0.363