Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   H7R28_RS02120 Genome accession   NZ_AP022069
Coordinates   482340..482807 (-) Length   155 a.a.
NCBI ID   WP_000340159.1    Uniprot ID   -
Organism   Escherichia coli strain WP4-W18-ESBL-09     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 477340..487807
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R28_RS02095 (WP4W18E09_04170) rpsG 477481..477951 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  H7R28_RS02100 (WP4W18E09_04180) fusA 478048..480162 (+) 2115 WP_000124703.1 elongation factor G -
  H7R28_RS02105 (WP4W18E09_04190) tuf 480233..481417 (+) 1185 WP_000031783.1 elongation factor Tu -
  H7R28_RS02110 (WP4W18E09_04200) bfd 481600..481794 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  H7R28_RS02115 (WP4W18E09_04210) bfr 481867..482343 (+) 477 WP_000675515.1 bacterioferritin -
  H7R28_RS02120 (WP4W18E09_04220) pilD 482340..482807 (-) 468 WP_000340159.1 A24 family peptidase Machinery gene
  H7R28_RS02125 (WP4W18E09_04230) rpsJ 483186..483497 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  H7R28_RS02130 (WP4W18E09_04240) rplC 483530..484159 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  H7R28_RS02135 (WP4W18E09_04250) rplD 484170..484775 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  H7R28_RS02140 (WP4W18E09_04260) rplW 484772..485074 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  H7R28_RS02145 (WP4W18E09_04270) rplB 485092..485913 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  H7R28_RS02150 (WP4W18E09_04280) rpsS 485930..486208 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  H7R28_RS02155 (WP4W18E09_04290) rplV 486223..486555 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  H7R28_RS02160 (WP4W18E09_04300) rpsC 486573..487274 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  H7R28_RS02165 (WP4W18E09_04310) rplP 487287..487697 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 17034.17 Da        Isoelectric Point: 8.1255

>NTDB_id=65606 H7R28_RS02120 WP_000340159.1 482340..482807(-) (pilD) [Escherichia coli strain WP4-W18-ESBL-09]
MDATLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=65606 H7R28_RS02120 WP_000340159.1 482340..482807(-) (pilD) [Escherichia coli strain WP4-W18-ESBL-09]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCAGCCCTACTTTTTTTCTGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACGTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTATCAAGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACATGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTATTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGCTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(11-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.882

81.935

0.368

Multiple sequence alignment