Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   H7R14_RS02060 Genome accession   NZ_AP022049
Coordinates   464020..464487 (-) Length   155 a.a.
NCBI ID   WP_000340159.1    Uniprot ID   -
Organism   Escherichia coli strain WP4-S17-ESBL-08     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 459020..469487
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R14_RS02035 (WP4S17E08_04050) rpsG 459161..459631 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  H7R14_RS02040 (WP4S17E08_04060) fusA 459728..461842 (+) 2115 WP_000124700.1 elongation factor G -
  H7R14_RS02045 (WP4S17E08_04070) tuf 461913..463097 (+) 1185 WP_000031783.1 elongation factor Tu -
  H7R14_RS02050 (WP4S17E08_04080) bfd 463280..463474 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  H7R14_RS02055 (WP4S17E08_04090) bfr 463547..464023 (+) 477 WP_000675515.1 bacterioferritin -
  H7R14_RS02060 (WP4S17E08_04100) pilD 464020..464487 (-) 468 WP_000340159.1 A24 family peptidase Machinery gene
  H7R14_RS02065 (WP4S17E08_04110) rpsJ 464866..465177 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  H7R14_RS02070 (WP4S17E08_04120) rplC 465210..465839 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  H7R14_RS02075 (WP4S17E08_04130) rplD 465850..466455 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  H7R14_RS02080 (WP4S17E08_04140) rplW 466452..466754 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  H7R14_RS02085 (WP4S17E08_04150) rplB 466772..467593 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  H7R14_RS02090 (WP4S17E08_04160) rpsS 467610..467888 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  H7R14_RS02095 (WP4S17E08_04170) rplV 467903..468235 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  H7R14_RS02100 (WP4S17E08_04180) rpsC 468253..468954 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  H7R14_RS02105 (WP4S17E08_04190) rplP 468967..469377 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 17034.17 Da        Isoelectric Point: 8.1255

>NTDB_id=65441 H7R14_RS02060 WP_000340159.1 464020..464487(-) (pilD) [Escherichia coli strain WP4-S17-ESBL-08]
MDATLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=65441 H7R14_RS02060 WP_000340159.1 464020..464487(-) (pilD) [Escherichia coli strain WP4-S17-ESBL-08]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCAGCCCTACTTTTTTTCTGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACGTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTATCAAGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACATGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTATTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGCTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(11-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.882

81.935

0.368

Multiple sequence alignment