Detailed information    

experimental Experimentally validated

Overview

Name   pstI   Type   Auxiliary factor
Locus tag   MMJJ_RS03465 Genome accession   NZ_CP026606
Coordinates   649522..650451 (-) Length   309 a.a.
NCBI ID   WP_104837703.1    Uniprot ID   A0A2L1C9R8
Organism   Methanococcus maripaludis strain DSM 2067     
Function   digesting foreign DNA   
Defense system

Function

Transformation of the M. maripaludis ∆MMJJ_06980 strain was tenfold more efficient than transformation of the parent strain suggesting that the native M. maripaludis PstI restriction activity was eliminated in the mutant.


Genomic Context

Location: 644522..655451
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMJJ_RS03440 (MMJJ_06920) - 645607..645873 (-) 267 WP_104837698.1 hypothetical protein -
  MMJJ_RS03445 (MMJJ_06940) - 646619..647167 (+) 549 WP_104837699.1 hypothetical protein -
  MMJJ_RS03450 (MMJJ_06950) - 647218..647619 (+) 402 WP_104837700.1 hypothetical protein -
  MMJJ_RS03455 (MMJJ_06960) - 647698..648720 (-) 1023 WP_104837701.1 Kiwa anti-phage protein KwaB-like domain-containing protein -
  MMJJ_RS03460 (MMJJ_06970) - 648724..649401 (-) 678 WP_104837702.1 hypothetical protein -
  MMJJ_RS03465 (MMJJ_06980) pstI 649522..650451 (-) 930 WP_104837703.1 BsuBI/PstI family type II restriction endonuclease Auxiliary factor
  MMJJ_RS03470 (MMJJ_06990) - 650469..651977 (-) 1509 WP_104837704.1 Eco57I restriction-modification methylase domain-containing protein -
  MMJJ_RS03475 (MMJJ_07000) - 651999..652886 (-) 888 WP_104837705.1 tyrosine-type recombinase/integrase -
  MMJJ_RS03480 (MMJJ_07010) - 652895..653443 (-) 549 WP_104837706.1 hypothetical protein -
  MMJJ_RS03485 (MMJJ_07020) - 653657..655030 (-) 1374 WP_104837707.1 radical SAM protein -

Sequence

Protein


Download         Length: 309 a.a.        Molecular weight: 36150.59 Da        Isoelectric Point: 4.8782

>NTDB_id=65 MMJJ_RS03465 WP_104837703.1 649522..650451(-) (pstI) [Methanococcus maripaludis strain DSM 2067]
MGKLDEALTILKKFELPVKQQNNRSAYTLLALANIQENSDWKDSEDNLVRIHDILLFISENYGYTYAENSRETIRRQTIH
QFEHAGIVERNRDDPKRPTNSGKTVYSLTPEFLEVLKTYETDDFEKSVEWFLENKTSLSEKYQGIREIHKISVKVDDTEL
EFSSGEHNELQKEIIEEFGPRFAGGSKVLYVGDTAHKNLYKLDEELEKLGIPMTKHDKLPDVVLYDEKNNWIYLIEAVTS
HGPVSDKRYYELEEMLEDCKCGKIYVSCFRDKATFKKYVADIAWDTEVWISENPDHMIHYNGDRFIGPR

Nucleotide


Download         Length: 930 bp        

>NTDB_id=65 MMJJ_RS03465 WP_104837703.1 649522..650451(-) (pstI) [Methanococcus maripaludis strain DSM 2067]
ATGGGAAAGTTAGACGAAGCTTTAACTATTTTAAAGAAATTTGAGCTTCCAGTGAAGCAGCAAAATAATAGAAGTGCATA
TACTCTTTTAGCACTTGCAAATATCCAAGAAAACAGTGATTGGAAAGATTCAGAAGATAATTTAGTTAGAATTCATGATA
TTTTATTGTTTATTTCTGAAAATTATGGATATACTTATGCTGAAAATAGCAGGGAAACAATAAGGCGGCAGACAATACAC
CAGTTTGAACATGCTGGAATTGTTGAAAGGAATCGAGATGATCCAAAAAGACCTACAAACAGTGGTAAAACAGTTTATTC
CTTAACTCCTGAATTTTTAGAAGTTTTAAAAACATACGAAACTGATGATTTTGAAAAGTCTGTTGAATGGTTTTTAGAGA
ATAAAACTTCACTTTCTGAAAAATATCAGGGAATTAGGGAAATTCATAAAATTTCAGTTAAAGTTGATGATACGGAACTT
GAATTTTCTTCTGGAGAACATAATGAATTACAAAAAGAAATTATTGAAGAATTTGGACCTAGATTTGCAGGTGGTTCAAA
AGTTCTTTATGTTGGGGATACCGCACATAAAAACCTTTATAAATTGGATGAAGAACTTGAAAAGCTTGGAATTCCAATGA
CTAAACATGATAAGCTTCCAGATGTTGTTTTGTATGATGAAAAGAATAACTGGATTTATTTAATTGAAGCAGTTACTTCT
CATGGCCCAGTTTCAGATAAACGGTACTATGAACTTGAAGAAATGCTTGAAGACTGTAAGTGTGGAAAAATATATGTGAG
CTGCTTTAGGGATAAAGCTACTTTCAAAAAATATGTTGCAGATATTGCATGGGATACTGAAGTTTGGATTTCAGAAAATC
CTGATCACATGATACACTATAATGGGGATAGATTTATCGGTCCTCGATAA

Domains

Predicted by InterproScan.

BsuBI/PstI restriction endonuclease HTH domain (BsuBI_PstI_RE_N)

(3-139)

BsuBI/PstI restriction endonuclease domain (BsuBI_PstI_RE)

(152-302)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L1C9R8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Dallas R Fonseca et al. (2023) Random transposon mutagenesis identifies genes essential for transformation in Methanococcus maripaludis. Molecular Genetics And Genomics : MGG 298(3):537-548. [PMID: 36823423]