Detailed information NTG0649882

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: L2Z29_RS01140
• Genome accession: NZ_CP091347
• Coordinates: 219084..220142 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain AB216-VUB
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 214084..225142

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L2Z29_RS01115 (L2Z29_01115)	aroK	214354..214923 (-)	570	WP_000643712.1	shikimate kinase AroK	-
L2Z29_RS01120 (L2Z29_01120)	pilQ	214935..217100 (-)	2166	WP_001017033.1	type IV pilus secretin PilQ family protein	Machinery gene
L2Z29_RS01125 (L2Z29_01125)	pilP	217168..217695 (-)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
L2Z29_RS01130 (L2Z29_01130)	pilO	217706..218446 (-)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
L2Z29_RS01135 (L2Z29_01135)	pilN	218443..219084 (-)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
L2Z29_RS01140 (L2Z29_01140)	pilM	219084..220142 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
L2Z29_RS01145 (L2Z29_01145)	ponA	220305..222860 (+)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
L2Z29_RS01150 (L2Z29_01150)	-	222878..223702 (-)	825	WP_002019666.1	putative RNA methyltransferase	-
L2Z29_RS01155 (L2Z29_01155)	-	223705..224037 (-)	333	WP_000169594.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=649882 L2Z29_RS01140 WP_002014066.1 219084..220142(-) (pilM) [Acinetobacter baumannii strain AB216-VUB]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=649882 L2Z29_RS01140 WP_002014066.1 219084..220142(-) (pilM) [Acinetobacter baumannii strain AB216-VUB]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTACTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTCTTTGGTGGCAAGCAACTTACGCTTGAAATTCAAAGTCGTTATGGCTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTAGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423