Detailed information NTG0648876

Bioinformatically predicted

Overview

• Name: addB
• Type: Machinery gene
• Locus tag: L3C11_RS02345
• Genome accession: NZ_CP091288
• Coordinates: 480599..484099 (-)
• Length: 1166 a.a.
• NCBI ID: WP_032874392.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain JJ47
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 475599..489099

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L3C11_RS02335 (L3C11_02335)	sbcD	475671..476843 (-)	1173	WP_032874394.1	exonuclease subunit SbcD	-
L3C11_RS02340 (L3C11_02340)	addA	476908..480612 (-)	3705	WP_032875181.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
L3C11_RS02345 (L3C11_02345)	addB	480599..484099 (-)	3501	WP_032874392.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
L3C11_RS02350 (L3C11_02350)	-	484240..484953 (+)	714	WP_079891127.1	DUF421 domain-containing protein	-
L3C11_RS02355 (L3C11_02355)	-	485022..485531 (+)	510	WP_014304752.1	ferritin-like domain-containing protein	-
L3C11_RS02360 (L3C11_02360)	-	485760..486380 (+)	621	WP_003155153.1	TetR/AcrR family transcriptional regulator	-
L3C11_RS02365 (L3C11_02365)	-	486396..487592 (+)	1197	WP_003155154.1	MFS transporter	-
L3C11_RS02370 (L3C11_02370)	-	487760..488917 (+)	1158	WP_014304750.1	AbrB family transcriptional regulator	-

Sequence

Protein

• Length: 1166 a.a.
• Molecular weight: 133367.85 Da
• Isoelectric Point: 5.3149

>NTDB_id=648876 L3C11_RS02345 WP_032874392.1 480599..484099(-) (addB) [Bacillus velezensis strain JJ47]
MGVEFLVGRSGSGKTRLIIDSIQDELRREPFGKPIIFLVPDQMTFLMEYELAKTPDIGGMIRAQVFSFSRLAWRVLQHTG
GMNRPFVTTTGVQMLLRKLIEEYKHEFKVYQKASDKTGFTEQVERMLTEFKRYCIEPEDVRRMAESGTASEYRGERMLSE
KLHDLGILYQQMERSLAGHYLHSEDYLTLLAQQIPLADVVKGARVYVDGFYQFTPQELRVLEQLIMHAEHVTFSLTADSP
SAEQAPDELDLFRMTGSTYYKLYQTAKELNADISCKELHGTKRHQHAPELACIESQYDVRPAAAYTGGQEAFTVMQAQNR
RAELEGIAREIQSLVRDGGYRYKDMAILIRQPEDYKDLLKEVFADYGLPYFIDGKASMQHHPLIEFIRSSLDVVKGNWRY
EAVFRCAKTELLFPLDQPEQKIREQVDQLENYCIAYGIKGERWTSGERFVYRRFVSLDEDFAQTDQEIEMEHMLNETREW
IAAPLVRLQNRMKKAKTVQSMAEALYLFLEDTDVPLKLDRKRQRAEEAGNMIEAQQHGQAWDAVIQLLEEFAGMMGEDEI
SLALFQQMLETGTESLHFSLIPPALDQVFVGNMDLSRMYGTSCTFVIGANDGVLPARPDENGVLSDDDREWLKAVGVELS
SAGRERLLDEHFLIYMALSSPSDRLYVSYPIADAEGKTLLPSIVVNRLGELFPDHQEKLSAADPEQVSEEEQLQYLVNKQ
VAQTYTASQLRLWTREYEISDVWWSAYNVLMKESDHRRAKKLFSSLFFRNEAKRLERPVSRQLYGEHIKGSVSRMEAFNA
CQFSHFASHGLQLKERQFFKLDAPDIGQLFHSSLKLISDRLREQKLEWRDLTKDQCRNFSYEAVERLAPKLQKEILLSSN
RHFYVKEKLQKIVTRVSGILSEHAKASGFVPVGLELGFGGSGPLPPLTFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDRGLDLAEVYYGLALQMLTYLDLSITHSEDWLGMKATPAGVLYFHIHDPMIQASLPMGLDEIEQEIFKKFKMKGL
LLGDREAISLMDTTLEEGRSNIVNAGLKKDGSLRSDSAAVSEQDFHLLTDHVRRTFEQAGEAITDGLVSITPYKLKDKTP
CTYCAFKSVCQFDESLKENEYRSLKAEKDGTILDWLKKEADDDANS

Nucleotide

• Length: 3501 bp

>NTDB_id=648876 L3C11_RS02345 WP_032874392.1 480599..484099(-) (addB) [Bacillus velezensis strain JJ47]
TTGGGTGTTGAGTTTTTAGTAGGCAGATCGGGGAGTGGGAAAACGAGGCTGATTATCGACAGCATTCAAGATGAACTGCG
CCGGGAGCCGTTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATATGAGCTTGCTAAAA
CGCCGGATATCGGCGGCATGATACGCGCTCAAGTGTTCAGTTTTTCGCGTTTGGCGTGGCGCGTGCTTCAGCATACCGGG
GGCATGAACCGCCCGTTTGTGACGACGACGGGTGTGCAGATGCTTTTAAGGAAACTGATTGAAGAATATAAGCACGAGTT
TAAAGTGTATCAAAAAGCGAGTGACAAAACCGGGTTCACTGAGCAGGTGGAACGGATGCTGACAGAGTTTAAGCGGTACT
GTATTGAGCCTGAAGACGTCCGCCGCATGGCGGAAAGCGGAACGGCGTCAGAATATCGCGGAGAGCGGATGCTGTCTGAA
AAACTTCATGATTTAGGCATTTTATATCAGCAGATGGAAAGAAGCCTCGCCGGACACTATTTGCATTCAGAGGACTATTT
GACGCTGCTCGCTCAGCAGATTCCTTTGGCGGACGTTGTAAAAGGCGCCCGTGTTTACGTCGACGGATTCTATCAATTTA
CGCCGCAGGAATTAAGGGTGCTGGAGCAGCTGATCATGCATGCTGAACATGTCACATTCTCGCTGACGGCGGACAGCCCT
TCGGCGGAACAGGCACCTGATGAGCTCGATCTGTTCAGAATGACGGGAAGCACATACTATAAGCTTTATCAGACGGCAAA
GGAATTGAATGCTGATATTTCCTGCAAGGAACTGCATGGCACGAAGCGGCATCAGCATGCACCGGAGCTTGCCTGTATAG
AGTCACAGTACGATGTGCGTCCCGCCGCGGCGTATACCGGCGGACAGGAAGCTTTTACCGTCATGCAGGCGCAAAACAGA
AGAGCTGAGCTTGAAGGTATTGCCCGGGAAATTCAATCGCTTGTCCGGGACGGAGGATACCGGTACAAAGATATGGCGAT
TCTCATCCGTCAGCCGGAAGATTATAAAGATTTGCTGAAGGAAGTGTTTGCCGATTACGGGCTTCCTTATTTTATTGACG
GAAAAGCTTCTATGCAGCATCACCCGCTTATTGAATTCATCCGTTCAAGCCTCGATGTCGTGAAGGGGAACTGGAGATAT
GAAGCAGTGTTCCGCTGTGCGAAAACCGAACTGTTATTCCCGCTTGATCAGCCGGAGCAGAAAATAAGAGAGCAGGTTGA
TCAGCTTGAAAATTACTGTATTGCCTACGGCATTAAAGGGGAGCGCTGGACAAGCGGCGAGCGGTTTGTATACAGGCGCT
TCGTATCGCTTGATGAGGATTTCGCGCAGACGGATCAGGAAATAGAGATGGAACACATGCTGAATGAGACGAGAGAGTGG
ATAGCCGCGCCCCTCGTCCGGCTTCAAAACCGGATGAAAAAAGCGAAAACGGTTCAAAGCATGGCGGAGGCTCTTTATCT
CTTTTTGGAAGATACCGATGTTCCACTGAAGCTGGACCGGAAAAGGCAGCGGGCCGAAGAGGCCGGAAACATGATCGAAG
CGCAGCAGCACGGCCAGGCGTGGGATGCCGTTATCCAGCTTCTCGAGGAGTTTGCCGGCATGATGGGTGAAGATGAAATA
TCATTGGCGCTGTTCCAGCAAATGCTTGAAACGGGAACGGAATCGCTTCATTTTTCTTTAATTCCGCCTGCTCTTGACCA
AGTATTTGTAGGGAATATGGATCTCTCAAGAATGTACGGCACGTCCTGCACATTCGTCATCGGGGCTAATGACGGCGTTC
TTCCGGCGCGCCCGGATGAGAACGGCGTGCTGTCTGATGATGACCGGGAGTGGCTGAAAGCGGTCGGAGTGGAGCTGTCT
TCCGCGGGGCGTGAGCGGCTGCTTGATGAGCATTTTCTCATCTATATGGCGCTGTCGAGTCCGTCTGACCGTCTTTATGT
GTCATACCCGATTGCCGATGCGGAAGGAAAAACGCTCCTGCCGTCAATCGTTGTAAACAGGCTTGGCGAGCTGTTTCCCG
ATCATCAGGAAAAGCTGTCAGCGGCTGATCCCGAGCAGGTAAGCGAAGAAGAGCAGCTTCAGTATCTCGTCAATAAACAG
GTGGCTCAGACATATACGGCAAGTCAGCTCAGGCTGTGGACAAGAGAGTACGAGATCAGTGACGTATGGTGGAGCGCATA
CAATGTGCTGATGAAAGAGTCTGATCACCGGCGTGCGAAAAAGCTGTTTTCAAGCCTGTTTTTCCGCAATGAGGCGAAGC
GTCTTGAACGGCCTGTTTCGCGCCAGCTTTACGGCGAGCATATTAAAGGAAGCGTCTCACGGATGGAGGCGTTTAATGCC
TGCCAGTTCTCTCATTTTGCATCTCACGGGCTTCAATTAAAAGAGCGGCAGTTTTTCAAGCTGGACGCGCCGGATATCGG
CCAGCTGTTTCACTCCAGCCTGAAGCTGATTTCAGACCGGCTTCGTGAGCAGAAGCTGGAATGGCGAGATCTGACGAAAG
ATCAGTGCCGGAATTTCTCCTATGAAGCGGTGGAACGGCTTGCGCCAAAGCTGCAAAAAGAGATTCTGCTCAGCTCAAAC
AGACACTTTTATGTGAAGGAAAAACTGCAGAAGATCGTCACGCGTGTTTCAGGCATATTAAGCGAGCATGCGAAGGCGAG
CGGTTTCGTCCCGGTCGGTCTTGAACTCGGTTTCGGCGGAAGCGGGCCGCTGCCCCCGCTCACTTTCACGCTGAAAAACG
GCTGTACAATGGAACTCGTCGGACGGATCGACCGCGTGGATAAAGCGGAAAGCTCAAAAGGCCTGCTGCTGAGAATCGTT
GATTATAAATCAAGCGACAGAGGGCTTGATCTGGCTGAGGTGTATTACGGGCTCGCTCTGCAAATGCTCACATACCTTGA
CTTATCGATTACACATTCCGAAGATTGGCTCGGTATGAAAGCGACACCGGCAGGCGTTTTGTATTTCCATATCCATGATC
CGATGATTCAGGCCAGTCTGCCGATGGGGCTTGATGAAATCGAGCAGGAAATTTTTAAGAAATTTAAAATGAAAGGCCTT
CTCCTCGGTGACCGGGAGGCGATCAGTCTGATGGACACGACTTTGGAGGAAGGGCGTTCAAATATCGTCAATGCCGGTTT
GAAAAAGGACGGGTCTTTAAGATCCGACTCAGCCGCGGTCAGCGAACAGGACTTTCATCTGCTGACAGACCATGTGCGGC
GGACATTTGAACAGGCTGGCGAAGCGATAACGGACGGGCTTGTGTCCATTACCCCGTATAAGCTGAAAGATAAGACGCCT
TGTACGTACTGTGCGTTTAAGTCGGTATGCCAGTTTGATGAGTCGCTTAAGGAAAATGAATACCGCAGCCTGAAAGCGGA
AAAAGACGGCACCATACTGGATTGGCTGAAAAAGGAGGCGGATGATGATGCAAATTCCTAA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (303-669)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (791-1131)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addB	Bacillus subtilis subsp. subtilis str. 168	83.448	100	0.834